Screening of independent prognostic long non-coding RNA for gastric cancer in TCGA

Abstract Background The global incidence of gastric cancer (GC) ranks the fourth among cancers and its 5-year survival is less than 25%. LncRNAs are vital regulators involved in pathological processes of cancer. It is urgent to screen the prognostic lncRNA in GC. Method Expression file and clinical data of GC were downloaded from TCGA. Differentially expressed lncRNAs were calculated by edger R package, followed by the prognosis analysis. COX analysis was conducted to compute the independent factor of GC. Potential signaling pathways that the screened lncRNAs enriched in were evaluated by gene set enrichment analysis (GSEA). At last, Pearson analysis was conducted to predict the possible mechanism of lncRNA in GC process. Result ENSG00000224363 was an unfavorable prognostic factor to OS (overall survival) and DFS (disease-free survival) of GC as COX regression analyzed. GSEA analysis indicated that ENSG00000224363 may regulate cell cycle, apoptosis and autophagy of GC cells. Conclusion LncRNA ENSG00000224363 is overexpressed in GC, serving as an independent unfavorable prognostic factor.