Developing A Third-Degree Burn Model Of Rat Using The Delphi Method

Background: The animal experiment plays an essential role in advances on research and treatment of burn. Currently, researchers often rely on personal experience or the literatures to complete the construction of animal disease models, which may lead to the lack of scientific rigor and wide range of animal disease models the reference value. The purposes of this study were aimed to establish a third-degree burn model of rat using a Delphi method in China to provide a reference. Methods: : Two rounds of Delphi expert consultation survey was conducted on experts (n=15) in this study, and then used the boundary value method to screen, modify and supplement the indicators. Next, determined the index weight by analytic hierarchy process, and finally the index system of the rat model of third-degree burn was established. Results: : Statistical analysis results show that the positive coefficients of the two rounds of expert consultation are 83.33% and 88.67% respectively. The expert authority coefficient values were 0.73 and 0.67 respectively, and the expert coordination coefficient test was P<0.001. According to experts' suggestion, the third-degree burn model of rats with 8 first-degree indexes, 14 second-degree indexes and 46 third-degree indexes was finally established. Conclusions: : According to the characteristics and quality requirements of my country's animal models, this study constructs a rat model of third degree burns, which is expected to expressively improve the overall proficiency of burn research quality.