Measurement of dehydration rate in vitro from SMILE-derived corneal stromal lenses

Measurement of dehydration rate in vitro from SMILE-derived corneal stromal lenses | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Measurement of dehydration rate in vitro from SMILE-derived corneal stromal lenses XiaoXuan Wang, Yulin Lei, Jie Hou, Mengting Li, Zhixing Ma, Lihong Xu This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4451415/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objective: To measure the expansion degree and dehydration rate of isolated corneal stromal lens and analyze the factors that affect dehydration rate to provide reference for analyzing the water content and fluid mechanics of the cornea and evaluating the optimal corneal exposure time Methods: Optical coherence tomography (OCT) was used to measure the thickness of the stromal lens obtained via small incision lenticule extraction (SMILE) surgery. The expansion degree of the stromal lens in balanced salt solution was calculated, and the change in the central thickness of the isolated stromal lens over time was recorded. The thickness–time dehydration fitting trend line was drawn, and the determination coefficient (R2) and dehydration rate were calculated. Whether differenceexisted in the dehydration rate of lenses with different diopter ranges was determined, and the effects of age, gender, and edema degree on dehydration rate were analyzed. Results: A significant difference was found between the thickness measured for the first time and the predicted thickness. The expansion degrees of the thin and thick lens groups were 2.05±0.33 and 1.91±0.19, respectively. The linear fitting trend line was drawn, and the mean R2 values of the thick and thin lens groups were 0.98423 and 0.988221, respectively. A difference existed in the dehydration rate between the thin and thick lens groups, and dehydration rate exhibited no correlation with age, sex, and edema degree. Conclusions: The water loss process is linear in 15 min, and water loss is slower in thick lens than in thin lens. Age, sex, and expansion degree exert no effect on dehydration rate. dehydration rate stromal lens SMILE surgery OCT Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4451415","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":309297925,"identity":"f511ce3f-917d-4cfd-80ea-a97403f1d540","order_by":0,"name":"XiaoXuan Wang","email":"","orcid":"","institution":"Binzhou Medical University","correspondingAuthor":false,"prefix":"","firstName":"XiaoXuan","middleName":"","lastName":"Wang","suffix":""},{"id":309297926,"identity":"c862e5b0-1f41-4aaa-af7b-61a77c047178","order_by":1,"name":"Yulin 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01:08:12","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4451415/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4451415/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":86810055,"identity":"d7bcc948-864a-477a-b238-86b3b0b69275","added_by":"auto","created_at":"2025-07-15 20:16:28","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":663334,"visible":true,"origin":"","legend":"","description":"","filename":"MeasurementofdehydrationrateinvitrofromSMILEderived.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4451415/v1_covered_ba1570ac-32ea-47f9-8239-9cb39d957146.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Measurement of dehydration rate in vitro from SMILE-derived corneal stromal lenses","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"dehydration rate, stromal lens, SMILE surgery, OCT","lastPublishedDoi":"10.21203/rs.3.rs-4451415/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4451415/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eObjective: To measure the expansion degree and dehydration rate of isolated corneal stromal lens and analyze the factors that affect dehydration rate to provide reference for analyzing the water content and fluid mechanics of the cornea and evaluating the optimal corneal exposure time\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eMethods: Optical coherence tomography (OCT) was used to measure the thickness of the stromal lens obtained via small incision lenticule extraction (SMILE) surgery. The expansion degree of the stromal lens in balanced salt solution was calculated, and the change in the central thickness of the isolated stromal lens over time was recorded. The thickness–time dehydration fitting trend line was drawn, and the determination coefficient (R2) and dehydration rate were calculated. Whether differenceexisted in the dehydration rate of lenses with different diopter ranges was determined, and the effects of age, gender, and edema degree on dehydration rate were analyzed.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eResults: A significant difference was found between the thickness measured for the first time and the predicted thickness. The expansion degrees of the thin and thick lens groups were 2.05±0.33 and 1.91±0.19, respectively. The linear fitting trend line was drawn, and the mean R2 values of the thick and thin lens groups were 0.98423 and 0.988221, respectively. A difference existed in the dehydration rate between the thin and thick lens groups, and dehydration rate exhibited no correlation with age, sex, and edema degree.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eConclusions: The water loss process is linear in 15 min, and water loss is slower in thick lens than in thin lens. Age, sex, and expansion degree exert no effect on dehydration rate.\u003c/p\u003e","manuscriptTitle":"Measurement of dehydration rate in vitro from SMILE-derived corneal stromal lenses","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-06-06 19:48:03","doi":"10.21203/rs.3.rs-4451415/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"7815c10b-7573-4bb1-a0c2-c784cf8466e1","owner":[],"postedDate":"June 6th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-07-15T20:08:13+00:00","versionOfRecord":[],"versionCreatedAt":"2024-06-06 19:48:03","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4451415","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4451415","identity":"rs-4451415","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}