sIL-1RII对IL-1α诱导子宫内膜异位症在位内膜基质细胞表达IL-8的影响

Objective: To explore the effects of soluble interleukin-1 receptor type Ⅱ (sIL-1RⅡ) on interleukin-1α (IL-1α)-induced expression of IL-8 in eutopic endometrial stromal cells (ESC) from patients with endometriosis (EMs). Methods: ESC from patients with EMs were cultured in vitro. Stromal cells were treated with different concentrations of IL-1α (0, 0.1, 1.0, 10, 100ng/ml) respectively for 24h. Enzyme-linked immuno-assay (ELISA) and RT-PCR were used to examine the expression of IL-8 in transcriptional and translational levels. The concentration of IL-1α which could remarkably enhance the expression of IL-S was used to stimulate ESC for different times. The levels of IL-8 were measured at each time point by RT-PCR and ELISA. Meanwhile, the effects of 1.0µg/ml of sIL-1RⅡon IL-1α-induced expression of IL-8 were detected. Results: ESC could slightly express IL-8 without stimulation. The levels of IL-8 protein and mRNA were both remarkably increased after IL-1α stimulation in a dose- and time- dependent manner. IL-8 expression induced by low concentration of IL-la was inhibited by 1.0µg/ml sIL-1RⅡ. Conclusion: IL-1α can induce IL-8 expression in eutopic ESC of EMs in a dose- and time-dependent manner. It may contribute to the pathogenesis and development of EMs. sIL-1RⅡ can partially antagonize the effect of IL-1α and may offer a rational therapy method for endometriosis.