Evaluation of Some Safety and Technological Properties of Yeast Strains Isolated from Aliha for Starter Culture Development.

Evaluation of Some Safety and Technological Properties of Yeast Strains Isolated from Aliha for Starter Culture Development. | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 29 August 2025 V1 Latest version Share on Evaluation of Some Safety and Technological Properties of Yeast Strains Isolated from Aliha for Starter Culture Development. Authors : Felix Kwashie Madilo 0000-0002-1997-6658 [email protected] , Angela Parry-Hanson Kunadu , Emmanuel Letsyo , and Kwaku Tano-Debrah Authors Info & Affiliations https://doi.org/10.22541/au.175647966.68789144/v1 124 views 71 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Fermentation remains one of the most widespread food preservation and transformation methods, with profound cultural, nutritional, and economic significance, particularly in Africa. Aliha is a traditional Ghanaian maize-based beverage, which is spontaneously fermented and valued for its cultural and traditional benefits in rural and peri-urban communities of Ghana. However, concerns about safety and quality attributes make upscaling difficult and unattractive. This study aims to select yeast strains with the best safety and technological potential for safe Aliha production and potential industrialization. To select the best yeast strains for industrial fermentation of Aliha , the yeast strains need to be assessed for their technological properties, including enzymatic activities (proteolytic, lipolytic, amylolytic, esterases, and β-glucosidases), and survival in acetate and lactate. The yeast cultures were also evaluated on their ability to overcome harsh intestinal environments like 3% bile salts, pH 2, pancreatic juice, pepsin, lysozyme, hemolysis, and screened for antimicrobial resistance. The results illustrate that Saccharomyces cerevisiae JYC2599, Pichia kudriavzevii CBS 5147 18S, and Pichia kudriavzevii CY902 were the best enzyme producers, depicting the highest recovery rates in harsh gastrointestinal conditions, inhibiting most pathogenic organisms, and resisting most antibiotics. They were all hemolytic negative (ℽ), which means they will not have any adverse effects on the host when they are considered as starters for food fermentation. Since poorly or uncharacterized microbial strains can pose health risks and limit the potential for product industrialization and commercial production, the above-qualified yeast strains will help produce Aliha with better qualities, leading to industrialization of the beverage. Supplementary Material File (final yeast manuscript.docx) Download 118.28 KB Information & Authors Information Version history V1 Version 1 29 August 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keyword saccharomyces Authors Affiliations Felix Kwashie Madilo 0000-0002-1997-6658 [email protected] Ho Technical University View all articles by this author Angela Parry-Hanson Kunadu University of Ghana View all articles by this author Emmanuel Letsyo Ho Technical University View all articles by this author Kwaku Tano-Debrah University of Ghana View all articles by this author Metrics & Citations Metrics Article Usage 124 views 71 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Felix Kwashie Madilo, Angela Parry-Hanson Kunadu, Emmanuel Letsyo, et al. Evaluation of Some Safety and Technological Properties of Yeast Strains Isolated from Aliha for Starter Culture Development.. Authorea . 29 August 2025. DOI: https://doi.org/10.22541/au.175647966.68789144/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. 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