Antiviral action of different molecules obtained from invertebrates against avian coronavirus

Antiviral action of different molecules obtained from invertebrates against avian coronavirus | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 26 July 2025 V1 Latest version Share on Antiviral action of different molecules obtained from invertebrates against avian coronavirus Authors : Ronaldo Zucatelli 0000-0001-6661-5110 [email protected] , Nascimento RM , Ana Carolina de Oliveira Fernandes , Almeida MIP , and Silva Junior PI Authors Info & Affiliations https://doi.org/10.22541/au.175350964.42430334/v1 168 views 102 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The few availability of antivirals for new strains of highly pathogenic viruses has become a serious public health problem that leads to the death of thousands of people annually. For this reason, the search for new products against these agents has become an urgent need. Many studies have been carried out with this aim. Among the multiple sources of research for new antibiotics and antivirals, bioprospecting for molecules obtained from invertebrates, or their products, has become an increasingly frequent option. Arthropods appeared on the planet around 350 million years ago and have been one of the beings with the greatest adaptability and resistance. Invertebrates have been found in all known ecosystems. Their survival for so long, in such different environments, is an indication that they have a very efficient protection system against environmental infections, despite not having a developed immune system like mammals. Historically, products obtained mainly from bees, such as honey and propolis, have been shown to be of great pharmacology importance, being used as antimicrobials, anti-inflammatory, antitumor, healing and several other functions. However, these molecules have also been obtained from other invertebrates, such as caterpillars and spiders. Previous studies by our group have demonstrated an intense antiviral and antimicrobial activity of molecules obtained from invertebrates. In this study, we identified and characterized compounds with potent antiviral effect against avian coronavirus in propolis from Scaptotrigona aff postica , hemolymph of Lonomia obliqua and from mygalin. Mygalin is an acyl polyamine, isolated from hemocytes of spider Acanthoscurria gomesiana . The antiviral assay was carried out by reducing infectious foci in cultures of infected cells and treated with these different substances. Propolis and crude hemolymph reduced avian coronavirus by an average of 256 x. Fraction purified of propolis and a synthetic peptide from hemolymph (transferrin), reduced the virus titer by an average 64 fold. Reduction of 8 fold was observed when 160µM of Mygalin was used. Virus reduction with 26 µM of synthetic mygalin was average of 16 times. The antiviral responses of the three substances were dose dependent. The virus titer reduction was 2 times more intense when the substances were added 1 hour before cell infection with the virus. The chemical characterization of the elements present in the propolis was carried out by liquid chromatography. Supplementary Material File (antiviral action aganst coronavirus jmv 25 07 25.docx) Download 407.78 KB Information & Authors Information Version history V1 Version 1 26 July 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords antiviral agents coronavirus disease control immune responses virucidal agents virus classification Authors Affiliations Ronaldo Zucatelli 0000-0001-6661-5110 [email protected] Instituto Butantan View all articles by this author Nascimento RM Instituto Butantan View all articles by this author Ana Carolina de Oliveira Fernandes Instituto Butantan View all articles by this author Almeida MIP Instituto Butantan View all articles by this author Silva Junior PI Instituto Butantan View all articles by this author Metrics & Citations Metrics Article Usage 168 views 102 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Ronaldo Zucatelli, Nascimento RM, Ana Carolina de Oliveira Fernandes, et al. Antiviral action of different molecules obtained from invertebrates against avian coronavirus. Authorea . 26 July 2025. 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