Fabrication of biocompatible artificial skin for full-thickness wound by co-culture of adipose mesenchymal stem cells-human keratinocytes on oxygen-producing nanofiber electrospun scaffold in rat animal model

Fabrication of biocompatible artificial skin for full-thickness wound by co-culture of adipose mesenchymal stem cells-human keratinocytes on oxygen-producing nanofiber electrospun scaffold in rat animal model | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Fabrication of biocompatible artificial skin for full-thickness wound by co-culture of adipose mesenchymal stem cells-human keratinocytes on oxygen-producing nanofiber electrospun scaffold in rat animal model Arezo Azari, Simzar Hosseinzadeh, Hojjat Allah Abbaszadeh, Azam Rahimpour, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5254281/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract In wound healing and skin regeneration, hypoxia is a significant limitation that delays this process. Lack of oxygen can lead to cell death and postpone tissue regeneration. Hence, eliminating the absence of oxygen by generating oxygen from an oxygen-generating scaffold can accelerate the skin's healing process. In our previous study, after making a scaffold with three components of polyurethane-polycaprolactone-gelatin-sodium bicarbonate, the scaffold was characterized, and the amount of oxygen release was measured, and in the next step, keratinocyte and mesenchymal cells, respectively, with a ratio of 70 to 30 were cultured. The morphology of the cells on the scaffold, the number of cells on the scaffold, and the bioavailability of the scaffold were further studied. Then, after ensuring the increase in the expression of skin-specific genes, it was transplanted into rats in the present study, and then, as a biocompatible skin substitute, its effect on the wound healing process for full-thickness skin defects was investigated in the rat model. At the end of 3,7,10, and 14 days after surgery, the macroscopic and histopathological characteristics were evaluated at the end of 14 days after surgery. Results show that our designed scaffold groups accelerate the wound healing process compared to the control group. Scaffold 2/5% SPC+ co-culture groups demonstrated more wound repair efficacy. Animal results show that the effective release of oxygen from the scaffold leads to the acceleration of wound healing, the increase of angiogenesis, and the formation of granulation tissue in the early stages of the healing process, which can increase its therapeutic potential. It is proposed for regenerative medicine and skin tissue engineering platforms. Therefore, our study supports using oxygen-releasing scaffolds as a potential strategy to accelerate skin regeneration. regenerative medicine mesenchymal stem cells skin generation Oxygengenerating scaffold wound healing Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Full Text Additional Declarations No competing interests reported. Tables are available in the Supplementary Files section. Supplementary Files tables.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5254281","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":366328676,"identity":"cf404a44-949e-4e93-9e2d-7e69369ac146","order_by":0,"name":"Arezo Azari","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA40lEQVRIiWNgGAWjYLCCBAjF+ABI8PCRooXZAKSFjRTL2CTAJCFl/P2Hn314wGCXz89/+Fjl1xw7GTYG5oePbuDRInHgmPGMBIZky5kNx9Juy25LBjqMzdg4B581BxuMgX5hNjA42GN2W3IbM1ALD5s0Pi3yh9k/A7XUG9gf5jErltxWT1iLwTEekC2HDQzYeMwYP247TFiL4RmeYoYEg+MGEmfYkqUZtx3nYWMm4Be588c3M/6oqDYABt3Bjz+3Vdvzszc/fIzX+xDnQShmHjBJUDkSYPxBiupRMApGwSgYMQAA2bY9qViUHk0AAAAASUVORK5CYII=","orcid":"","institution":"Shahid Beheshti University of Medical Sciences","correspondingAuthor":true,"prefix":"","firstName":"Arezo","middleName":"","lastName":"Azari","suffix":""},{"id":366328678,"identity":"c55427d6-01e8-4820-8a14-621fe8711bb6","order_by":1,"name":"Simzar Hosseinzadeh","email":"","orcid":"","institution":"Shahid Beheshti University of Medical Sciences","correspondingAuthor":false,"prefix":"","firstName":"Simzar","middleName":"","lastName":"Hosseinzadeh","suffix":""},{"id":366328679,"identity":"4f257202-02c7-48d7-9163-a0982c257fac","order_by":2,"name":"Hojjat Allah Abbaszadeh","email":"","orcid":"","institution":"Shahid Beheshti University of Medical Sciences","correspondingAuthor":false,"prefix":"","firstName":"Hojjat","middleName":"Allah","lastName":"Abbaszadeh","suffix":""},{"id":366328681,"identity":"86a2a00c-de2f-471c-a3f6-d6fe2bc995e6","order_by":3,"name":"Azam Rahimpour","email":"","orcid":"","institution":"Shahid Beheshti University of Medical Sciences","correspondingAuthor":false,"prefix":"","firstName":"Azam","middleName":"","lastName":"Rahimpour","suffix":""},{"id":366328683,"identity":"a692db40-9730-4f18-a4dc-02a532537f52","order_by":4,"name":"Lida Shahghasempour","email":"","orcid":"","institution":"Shahid Beheshti University of Medical Sciences","correspondingAuthor":false,"prefix":"","firstName":"Lida","middleName":"","lastName":"Shahghasempour","suffix":""},{"id":366328689,"identity":"940ef3a0-347c-44be-89ba-b17fbb64ada2","order_by":5,"name":"Roya Fattahi","email":"","orcid":"","institution":"Shahid Beheshti University of Medical Sciences","correspondingAuthor":false,"prefix":"","firstName":"Roya","middleName":"","lastName":"Fattahi","suffix":""}],"badges":[],"createdAt":"2024-10-13 07:53:10","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-5254281/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5254281/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":67282336,"identity":"4f9ec814-b805-493e-8365-02b3ba5f4693","added_by":"auto","created_at":"2024-10-23 09:08:40","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":2347239,"visible":true,"origin":"","legend":"\u003cp\u003eThe steps of wounding and suturing the groups\u003c/p\u003e","description":"","filename":"figure1.png","url":"https://assets-eu.researchsquare.com/files/rs-5254281/v1/924af204d88ce04fbb809c15.png"},{"id":67282302,"identity":"654128e6-4c87-4552-a08b-daa1697a3124","added_by":"auto","created_at":"2024-10-23 09:08:38","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":3422480,"visible":true,"origin":"","legend":"\u003cp\u003eMacroscopic evaluation of wound healing\u003c/p\u003e","description":"","filename":"Figure2.png","url":"https://assets-eu.researchsquare.com/files/rs-5254281/v1/1caadad84cc19648b305cff0.png"},{"id":67282320,"identity":"066201d1-efd0-4427-9c06-a58b369f950f","added_by":"auto","created_at":"2024-10-23 09:08:39","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":123971,"visible":true,"origin":"","legend":"\u003cp\u003eDiagram of percentage of wound closure (wound repair) on day 3 (****P \u0026lt; 0.0001)\u003c/p\u003e","description":"","filename":"Figure3.png","url":"https://assets-eu.researchsquare.com/files/rs-5254281/v1/923ad0b3a4a60f49b84c9f74.png"},{"id":67282326,"identity":"732ac334-954c-47da-b8e7-15710b115784","added_by":"auto","created_at":"2024-10-23 09:08:39","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":102107,"visible":true,"origin":"","legend":"\u003cp\u003eDiagram of percentage of wound closure (wound repair) on day 7 (****P \u0026lt; 0.0001)\u003c/p\u003e","description":"","filename":"Figure4.png","url":"https://assets-eu.researchsquare.com/files/rs-5254281/v1/c59964fc7e6ecd76ddbe9017.png"},{"id":67282273,"identity":"bd3d3395-b324-4cb1-8c4f-79fddc64b0e2","added_by":"auto","created_at":"2024-10-23 09:08:36","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":107562,"visible":true,"origin":"","legend":"\u003cp\u003eDiagram of percentage of wound closure (wound repair) on day 10 (****P \u0026lt; 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Lack of oxygen can lead to cell death and postpone tissue regeneration. Hence, eliminating the absence of oxygen by generating oxygen from an oxygen-generating scaffold can accelerate the skin's healing process. In our previous study, after making a scaffold with three components of polyurethane-polycaprolactone-gelatin-sodium bicarbonate, the scaffold was characterized, and the amount of oxygen release was measured, and in the next step, keratinocyte and mesenchymal cells, respectively, with a ratio of 70 to 30 were cultured. The morphology of the cells on the scaffold, the number of cells on the scaffold, and the bioavailability of the scaffold were further studied. Then, after ensuring the increase in the expression of skin-specific genes, it was transplanted into rats in the present study, and then, as a biocompatible skin substitute, its effect on the wound healing process for full-thickness skin defects was investigated in the rat model.\u003cbr\u003e\nAt the end of 3,7,10, and 14 days after surgery, the macroscopic and histopathological characteristics were evaluated at the end of 14 days after surgery. Results show that our designed scaffold groups accelerate the wound healing process compared to the control group. Scaffold 2/5% SPC+ co-culture groups demonstrated more wound repair efficacy.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eAnimal results show that the effective release of oxygen from the scaffold leads to the acceleration of wound healing, the increase of angiogenesis, and the formation of granulation tissue in the early stages of the healing process, which can increase its therapeutic potential. It is proposed for regenerative medicine and skin tissue engineering platforms. Therefore, our study supports using oxygen-releasing scaffolds as a potential strategy to accelerate skin regeneration.\u003c/p\u003e","manuscriptTitle":"Fabrication of biocompatible artificial skin for full-thickness wound by co-culture of adipose mesenchymal stem cells-human keratinocytes on oxygen-producing nanofiber electrospun scaffold in rat animal model","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-10-23 09:08:10","doi":"10.21203/rs.3.rs-5254281/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"4f68559a-5524-462a-9927-54117e237b7f","owner":[],"postedDate":"October 23rd, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-10-23T09:24:04+00:00","versionOfRecord":[],"versionCreatedAt":"2024-10-23 09:08:10","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-5254281","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-5254281","identity":"rs-5254281","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}