Mechanically disturbing trichomes enhances cadmium accumulation in Arabidopsis via malate-mediated stomatal opening

Mechanically disturbing trichomes enhances cadmium accumulation in Arabidopsis via malate-mediated stomatal opening | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 30 April 2025 V1 Latest version Share on Mechanically disturbing trichomes enhances cadmium accumulation in Arabidopsis via malate-mediated stomatal opening Authors : Juan Du , Xue Chang , Hongyang Wei , Tongxin Shen , Minyu Hao , Ning Liu , Zhiyan Cao , Jingao Dong , and Li Hong Zhou 0000-0002-2426-1067 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174598936.66419983/v1 191 views 98 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Phytoremediation of cadmium-contaminated soils requires efficient metal accumulation in plants. While mechanical stimulation of trichomes through brushing enhances shoot Cd 2+ concentration in Arabidopsis thaliana , the underlying mechanisms remain unknown. We demonstrate that brushing trichomes triggers increased stomatal aperture, elevated stomatal conductance, and higher transpiration rates, culminating in enhanced shoot Cd 2+ accumulation. These responses were trichome-specific and absent in the glabrous gl1-1 mutant. Mechanistically, the increase in stomatal aperture depends on MPK3/MPK6 and involves malic acid as a key regulator, operating independently of canonical ABA signalling. Our work reveals a novel mechanosensory pathway linking trichome stimulation to stomatal regulation and heavy metal accumulation. This pathway shows how the epidermal structures of plants orchestrate their physiological responses, offering new avenues for improving phytoremediation by targeting trichome-mediated stomatal control. Supplementary Material File (ms for np.doc) Download 43.19 MB Information & Authors Information Version history V1 Version 1 30 April 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords cd2+ malic acid signaling stomata stomatal aperture trichome Authors Affiliations Juan Du Hebei Agricultural University View all articles by this author Xue Chang Hebei Agricultural University View all articles by this author Hongyang Wei Hebei Agricultural University View all articles by this author Tongxin Shen Hebei Agricultural University View all articles by this author Minyu Hao Hebei Agricultural University View all articles by this author Ning Liu Hebei Agricultural University View all articles by this author Zhiyan Cao Hebei Agricultural University View all articles by this author Jingao Dong Hebei Agricultural University View all articles by this author Li Hong Zhou 0000-0002-2426-1067 [email protected] Hebei Agricultural University View all articles by this author Metrics & Citations Metrics Article Usage 191 views 98 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Juan Du, Xue Chang, Hongyang Wei, et al. Mechanically disturbing trichomes enhances cadmium accumulation in Arabidopsis via malate-mediated stomatal opening. Authorea . 30 April 2025. DOI: https://doi.org/10.22541/au.174598936.66419983/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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