Study on the role of miR-433-3p in the occurrence and development of endometriosis

Lulu Wang, Wang L, Bai J, Jing Bai, Xiaoqing Zhu, Zhu X
Nucleosides, nucleotides & nucleic acids · 2026 · pp. 1–16 · doi:10.1080/15257770.2026.2718818 · PMID:42627080 · W7203946303
article OA: closed public-domain-us
View on OpenAlex View on PubMed View at publisher

Abstract

This study aims to characterize the expression pattern and biological functions of miR-433-3p in endometriosis. Eutopic and ectopic endometrial tissues were collected from endometriosis patients, and the expression level of miR-433-3p was detected by RT-qPCR. Serum samples were also collected from endometriosis patients and healthy female controls, and the diagnostic value of miR-433-3p for endometriosis was evaluated by receiver operating characteristic curve. In cell experiments, CCK-8 and Transwell assays were performed to assess the effects of miR-433-3p on the proliferation, migration and invasion of endometrial stromal cells (ESCs). RT-qPCR was used to detect the expression levels of epithelial-mesenchymal transition (EMT) markers. A dual-luciferase reporter assay was conducted to verify the targeted binding relationship between miR-433-3p and CHL1. The results showed that the level of miR-433-3p in ectopic endometrium was significantly lower than that in eutopic endometrium. Moreover, circulating miR-433-3p levels in the serum of endometriosis patients were significantly lower than those in healthy controls. Serum miR-433-3p demonstrated excellent diagnostic efficacy for endometriosis, with an AUC of 0.8777, a sensitivity of 93.90%, and a specificity of 76.25%. In cells, overexpression of miR-433-3p significantly suppressed the proliferation, migration, invasion and EMT. Mechanistically, CHL1 was identified as a direct downstream target of miR-433-3p, and rescue experiments confirmed that restored CHL1 expression could partially reverse the inhibitory effect of miR-433-3p on the aggressive biological phenotype of ESCs. Based on the above research results, we propose the following hypothesis: In endometriosis, miR-433-3p may inhibit the proliferation and invasion of ESCs by targeting CHL1. Moreover, circulating miR-433-3p is expected to become a non-invasive candidate biomarker for endometriosis.

My notes (saved in your browser only)

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (41)

Source provenance

europepmc
last seen: 2026-08-25T06:10:03.373225+00:00
openalex
last seen: 2026-08-25T06:02:57.817192+00:00
pubmed
last seen: 2026-08-25T06:04:42.362026+00:00
License: public-domain-us · commercial use OK · attribution required
Courtesy of the U.S. National Library of Medicine