The Use of the Neoglycolipid-Based Oligosaccharide Microarray System in the Diagnosis of Endometriosis – Preliminary Study

Wojtyla C, Tołwiński I, Laudański P
2024 · W7025238174
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A preliminary study of eight endometriosis patients and controls found that neoglycolipid-based oligosaccharide microarrays revealed distinct IgM and IgG antibody binding patterns in peritoneal fluid, suggesting potential diagnostic utility for the condition.

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This preliminary study investigated the potential of glycomics to diagnose endometriosis by analyzing IgG and IgM antibody binding properties in plasma and peritoneal fluid samples. The multicenter research compared eight patients with endometriosis against eight matched controls using a neoglycolipid-based oligosaccharide microarray system to identify specific carbohydrate-binding differences. While significant variations in antibody binding were observed in peritoneal fluid, statistical significance was not maintained after correction for plasma samples, though effect sizes indicated promising trends. This paper is centrally about endometriosis — specifically exploring novel biomarkers for its diagnosis through glycan-antibody interactions.

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Abstract

Cezary Wojtyla,1,2 Ignacy Tołwiński,3 Piotr Laudański1,2,4 1Women’s Health Research Institute, Calisia University, Kalisz, Poland; 2OVIklinika Infertility Center, Warsaw, Poland; 3Warsaw Southern Hospital, Warsaw, Poland; 4Department of Obstetrics, Gynecology and Gynecological Oncology, Medical University of Warsaw, Warsaw, PolandCorrespondence: Cezary Wojtyla, Women’s Health Research Institute, Calisia University, Kalisz, Poland, Email [email protected]: Endometriosis presents diagnostic challenges, and there is a need for developing novel biomarkers with satisfactory specificity and sensitivity. Glycomics, exploring glycosylation changes in glycoproteins, offers potential solutions. The aim of this study was to analyze the carbohydrate-binding properties of IgG and IgM antibodies in the plasma and peritoneal fluid samples and to identify any differences in the presence and the specificities of anti-carbohydrate antibodies in the endometriosis patient and the controls.Methods: Multicenter study was conducted in Poland between 2018 and 2019. Plasma and peritoneal fluid samples were collected from women undergoing laparoscopic surgery. Endometriosis patients (n=8) and controls (n=8), matched for cycle phase and disease stage, were selected. The neoglycolipid-based oligosaccharide microarray system was used to investigate IgG and IgM antibody binding properties to glycan-related probes in biological materials.Results: In peritoneal fluid samples, IgM binding to the following probes was significantly higher in endometriosis: GSC-915-4 (new), LNFP-I, NeuAcα-(6’)LNnO (F1), B-like decaosylceramide, log10(GM1-penta), and log10(GSC-915-5). In a control group higher IgG binding to log10(Orsay-5-AO) was observed. In plasma samples, endometriosis showed higher IgG binding to log10(NeuAcα-(6’)LNnO (F1)) and lower IgG binding to Gal2GlcNAc(1-3)-AO. After Benjamin–Hochberg correction, differences were not significant. Effect sizes highlighted some glycan probes in both plasma and peritoneal fluid. Strong correlations were observed among binding to certain glycan probes.Conclusion: This preliminary study suggests glycomics’ potential contribution to endometriosis diagnosis and understanding of its pathophysiology. Neoglycolipid-based microarrays hold promise for non-invasive endometriosis diagnostic tools. Further investigations with larger cohorts are warranted to validate these findings and explore potential correlations with antibody levels in plasma and peritoneal fluid. Glycomics emerges as a valuable diagnostic asset in endometriosis research.Keywords: endometriosis, glycomics, diagnosis, microarray
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Journal of Inflammation Research (Feb 2024) The Use of the Neoglycolipid-Based Oligosaccharide Microarray System in the Diagnosis of Endometriosis – Preliminary Study Abstract Cezary Wojtyla,1,2 Ignacy Tołwiński,3 Piotr Laudański1,2,4 1Women’s Health Research Institute, Calisia University, Kalisz, Poland; 2OVIklinika Infertility Center, Warsaw, Poland; 3Warsaw Southern Hospital, Warsaw, Poland; 4Department of Obstetrics, Gynecology and Gynecological Oncology, Medical University of Warsaw, Warsaw, PolandCorrespondence: Cezary Wojtyla, Women’s Health Research Institute, Calisia University, Kalisz, Poland, Email [email protected]: Endometriosis presents diagnostic challenges, and there is a need for developing novel biomarkers with satisfactory specificity and sensitivity. Glycomics, exploring glycosylation changes in glycoproteins, offers potential solutions. The aim of this study was to analyze the carbohydrate-binding properties of IgG and IgM antibodies in the plasma and peritoneal fluid samples and to identify any differences in the presence and the specificities of anti-carbohydrate antibodies in the endometriosis patient and the controls.Methods: Multicenter study was conducted in Poland between 2018 and 2019. Plasma and peritoneal fluid samples were collected from women undergoing laparoscopic surgery. Endometriosis patients (n=8) and controls (n=8), matched for cycle phase and disease stage, were selected. The neoglycolipid-based oligosaccharide microarray system was used to investigate IgG and IgM antibody binding properties to glycan-related probes in biological materials.Results: In peritoneal fluid samples, IgM binding to the following probes was significantly higher in endometriosis: GSC-915-4 (new), LNFP-I, NeuAcα-(6’)LNnO (F1), B-like decaosylceramide, log10(GM1-penta), and log10(GSC-915-5). In a control group higher IgG binding to log10(Orsay-5-AO) was observed. In plasma samples, endometriosis showed higher IgG binding to log10(NeuAcα-(6’)LNnO (F1)) and lower IgG binding to Gal2GlcNAc(1-3)-AO. After Benjamin–Hochberg correction, differences were not significant. Effect sizes highlighted some glycan probes in both plasma and peritoneal fluid. Strong correlations were observed among binding to certain glycan probes.Conclusion: This preliminary study suggests glycomics’ potential contribution to endometriosis diagnosis and understanding of its pathophysiology. Neoglycolipid-based microarrays hold promise for non-invasive endometriosis diagnostic tools. Further investigations with larger cohorts are warranted to validate these findings and explore potential correlations with antibody levels in plasma and peritoneal fluid. Glycomics emerges as a valuable diagnostic asset in endometriosis research.Keywords: endometriosis, glycomics, diagnosis, microarray

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