Estimating the Photorespiratory CO2 Compensation Point and CO2 Release in the Light Using the Laisk Method Combined with Photosynthetic Theory

Estimating the Photorespiratory CO2 Compensation Point and CO2 Release in the Light Using the Laisk Method Combined with Photosynthetic Theory | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 10 May 2025 V1 Latest version Share on Estimating the Photorespiratory CO2 Compensation Point and CO2 Release in the Light Using the Laisk Method Combined with Photosynthetic Theory Authors : Darwin Leonel Moreno Echeverry 0000-0001-8767-8970 [email protected] , Miko Kirschbaum , Margaret Barbour , and Liyin Liang 0000-0001-9831-4793 Authors Info & Affiliations https://doi.org/10.22541/au.174685155.58181843/v1 315 views 137 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The photorespiratory CO 2 compensation point (Γ*) and the rate of CO 2 release in the light (D L ) are critical parameters for understanding the carbon dynamics of C 3 plants. These two parameters can be derived from the widely-used Laisk method as the intercept of linear regression lines fitted to net assimilation rates (A net ) versus chloroplast CO 2 partial pressures (C c ) obtained at different low-irradiance levels. However, photosynthetic theory indicates curvature in the A net -C c relationship which conflicts with the use of linear relationships for analysis. We, therefore, systematically evaluated the limitations of the use of linear relationships across temperatures from 5 to 40 °C and quantified the sensitivity of errors in Γ* and D L estimates to the selected C c range. We found that wide CO 2 ranges, especially when they exclude the actual Γ*, can introduce substantial biases in parameter estimation with linear regressions, particularly at lower temperatures. It can lead to marked underestimates of Γ*, and biologically unrealistic D L . We propose refining the Laisk method by using a photosynthesis model to analyse data. The model better represents the non-linear A net -C c relationship and yields consistent Γ* and D L estimates, regardless of the CO 2 range used. Supplementary Material File (morenoecheverry_main_manuscript_pce.docx) Download 804.59 KB Information & Authors Information Version history V1 Version 1 10 May 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords day-respiration fvcb-model photosynthesis: carbon reactions rday rl respiration Authors Affiliations Darwin Leonel Moreno Echeverry 0000-0001-8767-8970 [email protected] Landcare Research New Zealand Palmerston North View all articles by this author Miko Kirschbaum Landcare Research New Zealand Palmerston North View all articles by this author Margaret Barbour The University of Waikato School of Science View all articles by this author Liyin Liang 0000-0001-9831-4793 Landcare Research New Zealand Palmerston North View all articles by this author Metrics & Citations Metrics Article Usage 315 views 137 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Darwin Leonel Moreno Echeverry, Miko Kirschbaum, Margaret Barbour, et al. Estimating the Photorespiratory CO2 Compensation Point and CO2 Release in the Light Using the Laisk Method Combined with Photosynthetic Theory. Authorea . 10 May 2025. 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