Integrated analysis of OsGBSSI, OsBEIIb and OsGCD expression in moderately-high resistant starch japonica rice during grain filling

Integrated analysis of OsGBSSI, OsBEIIb and OsGCD expression in moderately-high resistant starch japonica rice during grain filling | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Integrated analysis of OsGBSSI, OsBEIIb and OsGCD expression in moderately-high resistant starch japonica rice during grain filling JiaoJiao Qian, Feng-Qin Zhang, Jing Su, Rui Wang, Dan-Ting Zhang, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6746157/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This study investigated the expression patterns of key starch-synthesis genes, such as OsGBSSI, OsBEIIb and OsGCD, in japonica rice varieties with moderately-high resistant starch (RS) (1.5% - 2%) content during the grain-filling stage. The screening of 139 japonica rice germplasm resources identified seven high-RS varieties for subsequent detailed analysis. The findings revealed that OsGBSSI expression and enzyme activity were significantly upregulated during the early and middle grain-filling stages in high-RS varieties, thus underscoring its crucial role in RS synthesis. In contrast, OsBEIIb expression and enzyme activity exhibited a wave-like pattern that indicated a complex regulatory function in balancing amylopectin and amylose synthesis. Finally, while OsGCD mRNA expression showed no significant correlation with RS content, its enzyme activity was consistently higher in high-RS varieties during the late grain-filling stage, hence suggesting a potential indirect role in RS synthesis. This study provides valuable insights into the molecular mechanisms governing RS accumulation in japonica rice and provide a theoretical basis for breeding high-RS rice varieties with enhanced nutritional quality. Resistant Starch (RS) Rice OsGBSSI OsBEIIb OsGCD Grain Filling Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6746157","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":472533620,"identity":"c0915e60-17db-4770-ad74-082edebafec6","order_by":0,"name":"JiaoJiao Qian","email":"","orcid":"","institution":"Ningxia University","correspondingAuthor":false,"prefix":"","firstName":"JiaoJiao","middleName":"","lastName":"Qian","suffix":""},{"id":472533621,"identity":"05dcea8a-40fd-4881-9e14-6cf4aa04c2b7","order_by":1,"name":"Feng-Qin Zhang","email":"","orcid":"","institution":"Ningxia 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Filling","lastPublishedDoi":"10.21203/rs.3.rs-6746157/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6746157/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"This study investigated the expression patterns of key starch-synthesis genes, such as OsGBSSI, OsBEIIb and OsGCD, in japonica rice varieties with moderately-high resistant starch (RS) (1.5% - 2%) content during the grain-filling stage. The screening of 139 japonica rice germplasm resources identified seven high-RS varieties for subsequent detailed analysis. The findings revealed that OsGBSSI expression and enzyme activity were significantly upregulated during the early and middle grain-filling stages in high-RS varieties, thus underscoring its crucial role in RS synthesis. In contrast, OsBEIIb expression and enzyme activity exhibited a wave-like pattern that indicated a complex regulatory function in balancing amylopectin and amylose synthesis. Finally, while OsGCD mRNA expression showed no significant correlation with RS content, its enzyme activity was consistently higher in high-RS varieties during the late grain-filling stage, hence suggesting a potential indirect role in RS synthesis. This study provides valuable insights into the molecular mechanisms governing RS accumulation in japonica rice and provide a theoretical basis for breeding high-RS rice varieties with enhanced nutritional quality.","manuscriptTitle":"Integrated analysis of OsGBSSI, OsBEIIb and OsGCD expression in moderately-high resistant starch japonica rice during grain filling","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-06-19 16:05:46","doi":"10.21203/rs.3.rs-6746157/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"585b4102-4e63-48a1-a7fe-b6177c924724","owner":[],"postedDate":"June 19th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-01-12T07:39:20+00:00","versionOfRecord":[],"versionCreatedAt":"2025-06-19 16:05:46","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6746157","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6746157","identity":"rs-6746157","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}