Isolation,purification and cultivation in vitro of stromal cells in eutopic endometrium and ectopic endometrium of human uterus

Objective:To establish the method of isolation and cultivation of stromal cells in eutopic endometrium and ectopic endometrium of cases with endometriosis and normal endometrium of healthy cases.Methods:Isolation, purification and cultivation in vitro were performed by segment enzymatic hydrolysis, screening filtration, sedimentation and adherent purification technique;observation under light microscope, mouse anti-human vimentin antibody, mouse anti-human cytokeratin monoclonal antibody were used for immunocytochemical staining and identification among 12 samples of normal endometrium, 9 samples of eutopic endometrium and 6 samples of ectopic endometrium.Results:Isolation and cultivation of 10 samples of normal endometrium, 6 samples of eutopic endometrium and 4 samples of ectopic endometrium were successful, the purity of stromal cells was up to 93%, and all the stromal cells could be transferred for six to eight generations.Conclusion:Segment enzymatic hydrolysis, screening filtration, sedimentation and adherent purification technique can obtain uterine stromal cells with high purity, which provide ideal experimental model for further investigating the molecular biological mechanism of occurrence and development of endometriosis.