APEX-seq: RNA subcellular localization by proximity labeling

Abstract We introduce APEX‑seq, a method for RNA sequencing based on direct proximity labeling of RNA using the peroxidase enzyme APEX2. APEX‑seq in nine distinct subcellular locales produced a nanometer-resolution spatial map of the human transcriptome as a resource, revealing extensive and exquisite patterns of localization for diverse RNA classes and transcript isoforms. APEX‑seq should be widely applicable to many systems, enabling comprehensive investigations of the spatial transcriptome.