Effect of nerve growth factor on stromal cell proliferation and estrogen receptor α expression of eutopic endometrial cells of adenomyosis uterine

Objective To investigate the effect of nerve growth factor on stromal cell proliferation and estrogen receptor α(ERα) expression of eutopic endometrial cells of adenomyosis uterine. Methods The eutopic endometrial cells were isolated, cultured and identified in vitro, then added to 10 ng/ml,50 ng/ml, 100 ng/ml NGF. The growth and proliferation of the stromal cells were measured by MTT assay; Westen-blotting was used to measure the changed ERa. Results 50 ng/mlNGF could accelerate the proliferation of eutopic endometrial stromal cells of adenomyosis uterine, it showed a significant difference compared to other group ( P 0. 05 ) ; The expression of ERa in eutopic endometrial stromal cells were significandy increased after the treatment of 50 ng/ml NGF( P 0. 01). Conclusion 50 ng/ml NGF can significandy accelerate the cell proliferation of eutopic endometrial stromal cells of adenomyosis uterine and increase the expression of ERα.