Discovery of a New Virus of the Family Tymoviridae Isolated from Mosquitoes of the Genus Mansonia in Brazil

Abstract A new virus, named Mutum virus, of the Family Tymoviridae was isolated from mosquitoes (Mansonia spp.). This virus was isolated in clone C6/36 cells. After complete sequencing, it presented a genome with 6,494 nt. The isolated virus is phylogenetically more related to two viruses isolated from Culex spp. mosquitoes: Ek Balam virus, isolated from Mexico, and Culex-originated Tymoviridae-like virus, isolated from China. Owing to the similarity of the protein coding domains, genomic organization, and phylogenetic analysis, we suggest that this virus belongs to a new genus, family Tymoviridae