Subcellular Systems Follow Onsager Reciprocity

Subcellular Systems Follow Onsager Reciprocity | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Subcellular Systems Follow Onsager Reciprocity Arie Horowitz This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3938235/v6 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 03 Mar, 2025 Read the published version in npj Biomedical Innovations → Version 6 posted You are reading this latest preprint version Show more versions Editorial Note 19 February, 2024. The /v1 preprint file has been replaced at the request of the author to reflect the current submission file from 14th February, 2024 for this journal submission. Editorial notes are used to provide important context regarding the topic of a preprint or to alert readers to potential issues concerning that preprint or a downstream publication associated with it. For more information on editorial notes, see our Editorial Policies . Abstract The complexity of prokaryotic and, exceedingly, of eukaryotic cells, challenges the implemen-tation of methodologies to quantify intracellular dynamics. Although stochastic thermodynam-ics can be used to effectively quantify the energetics of single proteins or complexes, there is no statistical approach to determine the macrostate of a whole cell from its subcellular mi-crostates. The objective of this study is to test if Onsager reciprocity, a coupling between ther-modynamic flows, can be established between time-dependent intracellular processes from their measurements in previous studies. A prototypical thermodynamic profile of the cell is for-mulated as a model system to enable representation of its sub systems as force-driven ther-modynamic flows. Onsager reciprocity is validated on the bacterial flagellum and ATP syn-thase. It is then tested on the putative coupling between the contractile ring and treadmilling of the cytoskeleton in bacterial binary fission and eukaryotic cytokinesis, with opposite out-comes. All thermodynamic flows and forces are formulated to comply with their canonical defi-nitions. Biological sciences/Systems biology/Dynamical systems Biological sciences/Computational biology and bioinformatics/Network topology Biological sciences/Computational biology and bioinformatics/Cellular signalling networks Biological sciences/Systems biology/Dynamic networks thermodynamics nonequilibrium energy macrostate quantification entropy Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Published Journal Publication published 03 Mar, 2025 Read the published version in npj Biomedical Innovations → Version 6 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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