Physicochemical Characterization of Thermodynamic Parameters of Hemin by Inverse Gas Chromatography and Investigation of Isomer Separation Ability

Physicochemical Characterization of Thermodynamic Parameters of Hemin by Inverse Gas Chromatography and Investigation of Isomer Separation Ability | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Physicochemical Characterization of Thermodynamic Parameters of Hemin by Inverse Gas Chromatography and Investigation of Isomer Separation Ability Ayşe Erdoğan Çakar This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6787406/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 30 Oct, 2025 Read the published version in Brazilian Journal of Chemical Engineering → Version 1 posted 5 You are reading this latest preprint version Abstract This study examines the retention behavior of various polar and non-polar probes on hemin, which is employed as a stationary phase, using the inverse gas chromatography technique at infinite dilution. By utilizing the retention behaviour of the probes on hemin, thermodynamic parameters such as The Flory-Huggins interaction parameters ( \(\:{\chi\:}_{12}^{\infty\:}\) ) and mole fraction activity coefficients ( \(\:{{\gamma\:}}_{1}^{\infty\:}\) ) were determined by inverse gas chromatography in a temperature range of 125–150°C. The results obtained showed that all the probes were poor solvents for hemin. In addition, the dispersive surface energy ( \(\:{\gamma\:}_{S}^{D}\) ) of Hemin was determined in the range of 30–55°C. For this, the Doris-Gray method (39.13 to 44.65 mJ/m 2 ) and the Schultz method (35.06 to 42.97 mJ/m 2 ) were used. Furthermore, the specific free enthalpy of the solvents on the hemin was determined. With the obtained data, it was determined that the surface of hemin showed an acidic structure ( \(\:{K}_{D}/{K}_{A}=0.30\) ). Furthermore, the ability of Hemin to separate various series of isomers was investigated in the range of 30–55°C and it was observed that hemin performed separation of these series of isomers. Hemin inverse gas chromatography isomer separation thermodynamic parameters Full Text Cite Share Download PDF Status: Published Journal Publication published 30 Oct, 2025 Read the published version in Brazilian Journal of Chemical Engineering → Version 1 posted Reviewers agreed at journal 16 Jun, 2025 Reviewers invited by journal 16 Jun, 2025 Editor invited by journal 10 Jun, 2025 Editor assigned by journal 10 Jun, 2025 First submitted to journal 09 Jun, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6787406","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":472189014,"identity":"47c81911-1462-477c-8be5-8ee22506a0b6","order_by":0,"name":"Ayşe Erdoğan Çakar","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABAUlEQVRIiWNgGAWjYPACZhDB+ECiAsRmbiBaC7OBxRkQxUi8FjaJyjawbfi18M9If/jg4x5rOXn3s4cNbs6rjeZvB2r5UbENpxaJGznGhjOepRsbnslLfDhz2/HcGYcZGxh7ztzGbc2NHDZpngOHEzc25BgbS247ltsA1MLM2IZbi/yN9Oe//4C09L8xk/4751jufEJaDG4kmDEzALXMl8gxk5BsqMndQEiL4Zk3xpI9B9KNDSTeAPGxA7kbgVoO4vOL3PH0hx9+HACGWH+O4QOJmrrceecPH3zwowKP9wUSoC48AKYOg8kDuNUDAT9UWr4BTNXhVTwKRsEoGAUjEwAAM/ZiZ+EWIjsAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0002-5568-3959","institution":"Yildiz Technical University: Yildiz Teknik Universitesi","correspondingAuthor":true,"prefix":"","firstName":"Ayşe","middleName":"Erdoğan","lastName":"Çakar","suffix":""}],"badges":[],"createdAt":"2025-05-30 20:29:40","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6787406/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6787406/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s43153-025-00610-y","type":"published","date":"2025-10-30T15:57:44+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":95039978,"identity":"db1937df-783f-496b-8d3d-0f825c0c05ea","added_by":"auto","created_at":"2025-11-03 16:06:49","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":649377,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6787406/v1_covered_c9e0f3f3-d50b-4007-9736-a11f1dafd341.pdf"}],"financialInterests":"","formattedTitle":"Physicochemical Characterization of Thermodynamic Parameters of Hemin by Inverse Gas Chromatography and Investigation of Isomer Separation Ability","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"brazilian-journal-of-chemical-engineering","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bjce","sideBox":"Learn more about [Brazilian Journal of Chemical Engineering](http://link.springer.com/journal/43153)","snPcode":"43153","submissionUrl":"https://www.editorialmanager.com/bjce/default2.aspx","title":"Brazilian Journal of Chemical Engineering","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Hemin, inverse gas chromatography, isomer separation, thermodynamic parameters","lastPublishedDoi":"10.21203/rs.3.rs-6787406/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6787406/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis study examines the retention behavior of various polar and non-polar probes on hemin, which is employed as a stationary phase, using the inverse gas chromatography technique at infinite dilution. By utilizing the retention behaviour of the probes on hemin, thermodynamic parameters such as The Flory-Huggins interaction parameters (\u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\:{\\chi\\:}_{12}^{\\infty\\:}\\)\u003c/span\u003e\u003c/span\u003e) and mole fraction activity coefficients (\u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\:{{\\gamma\\:}}_{1}^{\\infty\\:}\\)\u003c/span\u003e\u003c/span\u003e) were determined by inverse gas chromatography in a temperature range of 125\u0026ndash;150\u0026deg;C. The results obtained showed that all the probes were poor solvents for hemin.\u003c/p\u003e \u003cp\u003eIn addition, the dispersive surface energy (\u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\:{\\gamma\\:}_{S}^{D}\\)\u003c/span\u003e\u003c/span\u003e) of Hemin was determined in the range of 30\u0026ndash;55\u0026deg;C. For this, the Doris-Gray method (39.13 to 44.65 mJ/m\u003csup\u003e2\u003c/sup\u003e) and the Schultz method (35.06 to 42.97 mJ/m\u003csup\u003e2\u003c/sup\u003e) were used. Furthermore, the specific free enthalpy of the solvents on the hemin was determined. With the obtained data, it was determined that the surface of hemin showed an acidic structure (\u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\:{K}_{D}/{K}_{A}=0.30\\)\u003c/span\u003e\u003c/span\u003e). Furthermore, the ability of Hemin to separate various series of isomers was investigated in the range of 30\u0026ndash;55\u0026deg;C and it was observed that hemin performed separation of these series of isomers.\u003c/p\u003e","manuscriptTitle":"Physicochemical Characterization of Thermodynamic Parameters of Hemin by Inverse Gas Chromatography and Investigation of Isomer Separation Ability","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-06-18 12:03:30","doi":"10.21203/rs.3.rs-6787406/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"reviewerAgreed","content":"","date":"2025-06-16T21:10:30+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-06-16T20:54:59+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"Brazilian Journal of Chemical Engineering","date":"2025-06-10T12:43:04+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-06-10T10:38:03+00:00","index":"","fulltext":""},{"type":"submitted","content":"Brazilian Journal of Chemical Engineering","date":"2025-06-09T06:28:37+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"brazilian-journal-of-chemical-engineering","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bjce","sideBox":"Learn more about [Brazilian Journal of Chemical Engineering](http://link.springer.com/journal/43153)","snPcode":"43153","submissionUrl":"https://www.editorialmanager.com/bjce/default2.aspx","title":"Brazilian Journal of Chemical Engineering","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"c14ac793-c556-44a3-880e-53d60d7fc7e4","owner":[],"postedDate":"June 18th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2025-11-03T16:01:33+00:00","versionOfRecord":{"articleIdentity":"rs-6787406","link":"https://doi.org/10.1007/s43153-025-00610-y","journal":{"identity":"brazilian-journal-of-chemical-engineering","isVorOnly":false,"title":"Brazilian Journal of Chemical Engineering"},"publishedOn":"2025-10-30 15:57:44","publishedOnDateReadable":"October 30th, 2025"},"versionCreatedAt":"2025-06-18 12:03:30","video":"","vorDoi":"10.1007/s43153-025-00610-y","vorDoiUrl":"https://doi.org/10.1007/s43153-025-00610-y","workflowStages":[]},"version":"v1","identity":"rs-6787406","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6787406","identity":"rs-6787406","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}