Assessing the causal relationship between CRP,IL-1α, IL-1β, and IL-6 levels and intervertebral discdegeneration: a two-sample Mendelianrandomization study

Assessing the causal relationship between CRP,IL-1α, IL-1β, and IL-6 levels and intervertebral discdegeneration: a two-sample Mendelianrandomization study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Assessing the causal relationship between CRP,IL-1α, IL-1β, and IL-6 levels and intervertebral discdegeneration: a two-sample Mendelianrandomization study Xin-an Yan, Erdong Shen, Aiyong Cui, Fengjin Zhou, Yan Zhuang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4232113/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 10 Oct, 2024 Read the published version in Scientific Reports → Version 1 posted 12 You are reading this latest preprint version Abstract Growing research has suggested an association between chronic inflammation and Intervertebral disc degeneration (IVDD),but whether there is a causal effect remains unknown. This study adopted two-sample Mendelian randomization (MR) approachto explore the etiological role of chronic inflammation in IVDD risk. Here, summary statistics for C-reactive protein (CRP),interleukin (IL)-1α, IL-1β, IL-6 expression and IVDD were obtained from genome-wide association studies (GWAS) of Europeanancestry. MR analyses were conducted by using inverse variance weighted (IVW), Wald Ratio, weighted median, and MR-Eggermethod. Sensitivity analyses were conducted to assess the robustness of the results. The MR analyses suggested a lackof causal association of CRP, IL-6 , and IL-1α levels on IVDD (CRP-IVDD: odds ratio [OR] = 0.97, 95% confidence interval[CI] : 0.86-1.09, P = 0.583; IL-6-IVDD: OR =1.04, 95% CI: 0.86-1.27, P = 0.679; IL-1α-IVDD: OR=1.09, 95%CI : 1.00-1.18,P=0.058). However, there was a sign of a connection between genetically elevated IL-1β levels and a decreased IVDDincidence(OR=0.87, 95%CI: 0.77-0.99, P=0.03). Our findings suggest a connection between IL-1β levels and the risk of IVDD.However, due to the support of only one SNP, heterogeneity and pleiotropy tests cannot be performed, the specific underlyingmechanisms warrant further investigation. Biological sciences/Immunology/Cytokines Biological sciences/Immunology/Inflammation Health sciences/Diseases Full Text Additional Declarations No competing interests reported. Supplementary Files STROBEMRchecklistXinanYanetal.docx SupplementaryMaterialsXinanYanetal.xlsx Cite Share Download PDF Status: Published Journal Publication published 10 Oct, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 13 Aug, 2024 Reviews received at journal 12 Aug, 2024 Reviewers agreed at journal 27 Jul, 2024 Reviews received at journal 19 Jul, 2024 Reviewers agreed at journal 09 Jul, 2024 Reviewers agreed at journal 09 May, 2024 Reviewers agreed at journal 08 May, 2024 Reviewers invited by journal 06 May, 2024 Editor assigned by journal 06 May, 2024 Editor invited by journal 18 Apr, 2024 Submission checks completed at journal 18 Apr, 2024 First submitted to journal 07 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4232113","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":293659780,"identity":"484ff596-4952-4e52-b352-1bc8e9c9fd6c","order_by":0,"name":"Xin-an Yan","email":"","orcid":"","institution":"Department of Pelvic and Acetabular Surgery, HongHui Hospital, Xi’an Jiaotong University","correspondingAuthor":false,"prefix":"","firstName":"Xin-an","middleName":"","lastName":"Yan","suffix":""},{"id":293659781,"identity":"cfd7d05e-a3cd-428b-b016-24b7828a833d","order_by":1,"name":"Erdong Shen","email":"","orcid":"","institution":"Department of 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