Dynamic high-pressure microjet combined with tyrosinase modify the complex system of pea protein and chlorogenic acid

Dynamic high-pressure microjet combined with tyrosinase modify the complex system of pea protein and chlorogenic acid | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Dynamic high-pressure microjet combined with tyrosinase modify the complex system of pea protein and chlorogenic acid Yajie Li, Yidan Ni, Tingting Guo, Haimei Bai, Qinshuo Han, Jiayi Li, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4250430/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 3 You are reading this latest preprint version Abstract At present, the phenomenon of polyphenol substances represented by chlorogenic acid combined with pea protein to form food processing raw materials is increasingly common in the food processing industry. The purpose of this study is to modify pea protein (PP)/chlorogenic acid (CA) composite solution by DHPM and tyrosinase (Tyr) in order to further obtain better properties, so as to solve the problem of limited application of natural pea protein. And, the influences of Tyr and DHPM on the structure and functional properties of PP/CA complex under different treatment conditions, and the mechanism and effect of Tyr's influence in the PP/CA binary system were performed. The results showed that compared with PP/CA liquid phase without Tyr and DHPM treatment, for PP/CA treated with Tyr and DHPM, the color changed less, the cross-linking effect was the best, and the structure of the composite liquid was stable. DHPM decreased the emulsifying activity of protein, while Tyr significantly increased the emulsifying activity. The microstructure, secondary structure and tertiary structure of the modified protein were changed. The microstructure showed a relatively complete sheet, and the hydrogen bond interaction was enhanced. The content of sulfhydryl group was significantly reduced by 21.14%, and the content of disulfide bond was significantly increased by 13.40%. This study provides a thought for PP modification. Theoretically, it can be applied to the preparation of emulsions and the inclusion of functional substances. A theoretical basis for the development of high-value protein products. Pea protein Tyrosinase Dynamic high pressure microjet Chlorogenic acid Composite modification Full Text Additional Declarations No competing interests reported. Supplementary Files Graphicalabstract.jpg Highlights.docx Cite Share Download PDF Status: Under Review Version 1 posted Editor assigned by journal 15 Apr, 2024 Submission checks completed at journal 14 Apr, 2024 First submitted to journal 11 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4250430","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":291219482,"identity":"c77439be-72e2-4f9a-9cd6-47037135d8e1","order_by":0,"name":"Yajie Li","email":"","orcid":"","institution":"Shandong University of Technology","correspondingAuthor":false,"prefix":"","firstName":"Yajie","middleName":"","lastName":"Li","suffix":""},{"id":291219483,"identity":"d2b1833a-61dd-4314-b46e-cd1afd2948cc","order_by":1,"name":"Yidan Ni","email":"","orcid":"","institution":"Shandong University of 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