Protein language models accelerate the discovery of Plastic-Degrading Enzymes

Abstract Plastic pollution presents a critical environmental challenge, necessitating innovative and sustainable solutions. In this context, biodegradation using microorganisms and enzymes offers an environmentally friendly alternative. This work introduces an AI-driven frame-work that integrates machine learning (ML) and generative models to accelerate the discovery and design of plastic-degrading enzymes. By leveraging pre-trained protein language models and curated datasets, we developed seven ML-based binary classification models to identify enzymes targeting specific plastic substrates, achieving an average accuracy of 89%. The framework was applied to over 6,000 enzyme sequences from the RemeDB to classify enzymes targeting diverse plastics, including PET, PLA, and Nylon. Besides, generative learning strategies combined with trained classification models in this work were applied for de novo generation of PET-degrading enzymes. Structural bioinformatics validated potential candidates through in-silico analysis, highlighting differences in physicochemical properties between generated and experimentally validated enzymes. Moreover, generated sequences exhibited lower molecular weights and higher aliphatic indices, features that may enhance interactions with hydrophobic plastic substrates. These findings highlight the utility of AI-based approaches in enzyme discovery, providing a scalable and efficient tool for addressing plastic pollution. Future work will focus on experimental validation of promising candidates and further refinement of generative strategies to optimize enzymatic performance. Competing Interest Statement The authors have declared no competing interest.