子宫内膜异位症患者中CD19(上标 +)CD5(上标 +)B细胞及BAFF变化的研究

Objective To explore the changes of frequency, BAFF expression and sensitivity to apoptosis of CD19(superscript +)CD5(superscript +)B cells in endometriosis patients and investigate the relationship between CD19(superscript +)CD5(superscript +)B cells and endometriosis. Methods Flow cytometry and quantitative PCR were used to assess the frequency, BAFF expression and sensitivity to apoptosis of CD19(superscript +)CD5(superscript +)B cells in the peripheral blood, peritoneal fluids and endometrium biopsies of 39 patients with endometriosis and 31 patients with myoma of uterus (control subjects). Results: All 39 endometriosis patients had higher frequencies (all P＜0.01) of CD19(superscript +)CD5(superscript +)B cells in the peripheral blood, peritoneal fluids and endometrium biopsies than that in 31 control subjects. CD19(superscript +)CD5(superscript +)B cells from endometriosis patients expressed higher BAFF, and were more resistant to CD95L-induced apoptosis than the cells from control subjects (P＜0.01). Conclusion CD19(superscript +)CD5(superscript +)B cells might play an important role in the pathogenesis of endometriosis through higher BAFF expression and more resistance to CD95L-induced apoptosis.