Vasopressinergic sexual dimorphism: Sex chromosome complement and organizational hormonal effects

Vasopressinergic sexual dimorphism: Sex chromosome complement and organizational hormonal effects | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Vasopressinergic sexual dimorphism: Sex chromosome complement and organizational hormonal effects Dadam Florencia María, Gonzalez Lihue, Vivas Laura, Godino Andrea, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-2429792/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract This study aimed to analyze the role of the sex chromosomes (SCC:XX/XY) and the interaction with organizational hormonal effects on Avp gene expression at the supraoptic (SON) and paraventricular nuclei (PVN) due to water deprivation-osmotic challenge, as well as on the vasopressinergic sexually dimorphic antidiuretic and pressor responses. For this purpose, we used gonadectomized (GDX) transgenic mice of the "four core genotypes" model, in which the effect of gonadal sex and SCC are dissociated. We evaluated a) mRNA Avp gene expression at the SON and PVN at baseline and after 24-hour water deprivation, b) desmopressin antidiuretic response and renal Avpr2 mRNA expression, c) vasopressin-induced bradycardic baroreflex regulation, and d) the pressor and bradycardic responses induced by continuous vasopressin infusion. Our results indicate, in absence of activational hormonal effects, an influence of SCC on basal Avp gene expression at the SON {FSCC (1,6) = 5.44, p<0.05}. Regardless of the organizational hormonal factor, male and female mice with XX-SCC showed a higher basal expression than those with XY-SCC. Furthermore, after 24 h of the osmotic-water deprivation challenge, a significant effect of the interaction between treatment and SCC was observed {F (1,13) = 5.91 p<0.05}. While water deprivation in XY-SCC mice (XY-male-DEP and XY-female-DEP) resulted in an increase in mRNA Avp expression compared to their respective control groups (XY-male-CON and XY-female-CON), SCC-XX mice (XX-Male/GDXDEP and XX-Female/GDX-DEP) showed similar levels of mRNA Avp expression than those reported for their control groups (XX-Male /GDX-CON and XX-Female/GDX--CON). In contrast, a significant interaction of the SCC, organizational hormonal, and treatment factors was observed at the PVN, revealing an increase in the expression of Avp gene expression in the XY-Male/GDX-DEP group. Furthermore, our results demonstrate that, although desmopressin treatment induced the expected antidiuretic effect {F(1,37)=439.63, p≤0,05} in both males and females, no SCC or organizational hormonal effects were observed on the antidiuretic or on renal Avpr2 mRNA expression in absence of activational hormonal influences. Regarding blood pressure regulation, our data also reveal an interplay of organizational hormonal and SCC factors in the homeostatic mechanisms involved in the short {F(5,125)=2.50;p<0.05} and medium-term {F(6,144)=3.891,p<0.005} vasopressin -blood pressure regulation. The analysis of vasopressin-bradycardic baroreflex responses showed a facilitated bradycardic baroreflex response in XX-Females/GDX compared to that reported for the other genotypes (XY-Male /GDX, XX-Male /GDX and XY-Female /GDX). Finally, statistical analysis of the changes in mean arterial pressure due to continuous vasopressin infusion demonstrated that vasopressin infusion resulted in an increase in the percentage change in MAP in all genotypes as expected, but a sustained blood pressure increase was observed in XX-Male/GDX mice compared to XY-male/GDX mice and to female mice (both XX-Female/GDX and XY-Female/GDX). Our data may contribute to understanding the hydromineral and blood pressure regulation of the complex interplay between vasopressin and SCC/organizational hormonal backgrounds. Physiology Vasopressin sexual dimorphism sex chromosome complement organizational hormonal effect Avp gene expression water deprivation Avpr2 vasopressin receptor antidiuretic effect blood pressure regulation Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-2429792","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":168167247,"identity":"bb48ce3a-5624-415e-bf91-94d71d84d631","order_by":0,"name":"Dadam Florencia María","email":"","orcid":"","institution":"Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba","correspondingAuthor":false,"prefix":"","firstName":"Dadam","middleName":"Florencia","lastName":"María","suffix":""},{"id":168167246,"identity":"f5480b42-e1a7-4cc6-b41c-beffbcf5d9b0","order_by":1,"name":"Gonzalez 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interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eVasopressinergic sexual dimorphism: Sex chromosome complement and organizational hormonal effects\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Vasopressin, sexual dimorphism, sex chromosome complement, organizational hormonal effect, Avp gene expression, water deprivation, Avpr2 vasopressin receptor, antidiuretic effect, blood pressure regulation","lastPublishedDoi":"10.21203/rs.3.rs-2429792/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-2429792/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis study aimed to analyze the role of the sex chromosomes (SCC:XX/XY) and the interaction \u0026nbsp;with \u0026nbsp;organizational \u0026nbsp;hormonal \u0026nbsp;effects \u0026nbsp;on \u0026nbsp;Avp \u0026nbsp;gene \u0026nbsp;expression \u0026nbsp;at \u0026nbsp;the supraoptic \u0026nbsp;(SON) \u0026nbsp;and \u0026nbsp;paraventricular \u0026nbsp;nuclei \u0026nbsp;(PVN) \u0026nbsp;due \u0026nbsp;to \u0026nbsp;water deprivation-osmotic challenge, as well as on the vasopressinergic sexually dimorphic antidiuretic and pressor responses. For this purpose, we used gonadectomized (GDX) transgenic mice of the \"four core genotypes\" model, in which the effect of gonadal sex and SCC are dissociated. We evaluated a) mRNA Avp gene expression at the SON and PVN at baseline and after 24-hour \u0026nbsp;water \u0026nbsp;deprivation, \u0026nbsp;b) \u0026nbsp;desmopressin \u0026nbsp;antidiuretic \u0026nbsp;response \u0026nbsp;and \u0026nbsp;renal \u0026nbsp;Avpr2 mRNA expression, c) vasopressin-induced bradycardic baroreflex regulation, and d) the pressor and bradycardic responses induced by continuous vasopressin infusion. \u0026nbsp;\u003c/p\u003e\n\u003cp\u003eOur results indicate, in absence of activational hormonal effects, an influence of SCC on basal Avp gene expression at the SON {FSCC (1,6) = 5.44, p\u0026lt;0.05}. Regardless of the organizational hormonal factor, male and female mice with XX-SCC showed a higher basal expression than those with XY-SCC. Furthermore, after 24 h of the osmotic-water deprivation challenge, a significant effect of the interaction between treatment and SCC was observed {F (1,13) = 5.91 p\u0026lt;0.05}. While water deprivation in XY-SCC mice (XY-male-DEP \u0026nbsp;and \u0026nbsp;XY-female-DEP) \u0026nbsp;resulted \u0026nbsp;in \u0026nbsp;an \u0026nbsp;increase \u0026nbsp;in \u0026nbsp;mRNA \u0026nbsp;Avp \u0026nbsp;expression compared to their respective control groups (XY-male-CON and XY-female-CON), SCC-XX mice (XX-Male/GDXDEP and XX-Female/GDX-DEP) showed similar levels of mRNA Avp expression than those reported for their control groups (XX-Male /GDX-CON and XX-Female/GDX--CON). \u0026nbsp;In contrast, a significant interaction of the SCC, organizational hormonal, and treatment factors was observed at the PVN, revealing an increase in the expression of Avp gene expression in the XY-Male/GDX-DEP group. Furthermore, our results \u0026nbsp;demonstrate \u0026nbsp;that, \u0026nbsp;although \u0026nbsp;desmopressin \u0026nbsp;treatment \u0026nbsp;induced \u0026nbsp;the \u0026nbsp;expected antidiuretic \u0026nbsp;effect {F(1,37)=439.63, \u0026nbsp;p≤0,05} \u0026nbsp;in \u0026nbsp;both \u0026nbsp;males \u0026nbsp;and \u0026nbsp;females, \u0026nbsp;no \u0026nbsp;SCC \u0026nbsp;or organizational \u0026nbsp;hormonal \u0026nbsp;effects \u0026nbsp;were \u0026nbsp;observed \u0026nbsp;on \u0026nbsp;the \u0026nbsp;antidiuretic \u0026nbsp;or \u0026nbsp;on \u0026nbsp;renal \u0026nbsp;Avpr2 mRNA expression in absence of activational hormonal influences. \u0026nbsp;Regarding blood pressure regulation, our data also reveal an interplay of organizational hormonal \u0026nbsp;and \u0026nbsp;SCC \u0026nbsp;factors \u0026nbsp;in \u0026nbsp;the \u0026nbsp;homeostatic \u0026nbsp;mechanisms \u0026nbsp;involved \u0026nbsp;in \u0026nbsp;the \u0026nbsp;short {F(5,125)=2.50;p\u0026lt;0.05} and medium-term {F(6,144)=3.891,p\u0026lt;0.005} vasopressin -blood pressure \u0026nbsp;regulation. \u0026nbsp;The \u0026nbsp;analysis \u0026nbsp;of \u0026nbsp;vasopressin-bradycardic \u0026nbsp;baroreflex \u0026nbsp;responses showed a facilitated bradycardic baroreflex response in XX-Females/GDX compared to that reported for the other genotypes (XY-Male /GDX, XX-Male /GDX and XY-Female /GDX). \u0026nbsp;Finally, \u0026nbsp;statistical \u0026nbsp;analysis \u0026nbsp;of \u0026nbsp;the \u0026nbsp;changes \u0026nbsp;in \u0026nbsp;mean \u0026nbsp;arterial \u0026nbsp;pressure \u0026nbsp;due \u0026nbsp;to continuous vasopressin infusion demonstrated that vasopressin infusion resulted in an increase in the percentage change in MAP in all genotypes as expected, but a sustained blood \u0026nbsp;pressure \u0026nbsp;increase \u0026nbsp;was \u0026nbsp;observed \u0026nbsp;in \u0026nbsp;XX-Male/GDX \u0026nbsp;mice \u0026nbsp;compared \u0026nbsp;to \u0026nbsp;XY-male/GDX mice and to female mice (both XX-Female/GDX and XY-Female/GDX).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eOur \u0026nbsp;data \u0026nbsp;may \u0026nbsp;contribute \u0026nbsp;to \u0026nbsp;understanding \u0026nbsp;the \u0026nbsp;hydromineral \u0026nbsp;and \u0026nbsp;blood \u0026nbsp;pressure regulation \u0026nbsp;of \u0026nbsp;the \u0026nbsp;complex \u0026nbsp;interplay \u0026nbsp;between \u0026nbsp;vasopressin \u0026nbsp;and \u0026nbsp;SCC/organizational hormonal backgrounds.\u003c/p\u003e","manuscriptTitle":"Vasopressinergic sexual dimorphism: Sex chromosome complement and organizational hormonal effects","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2024-05-09 20:43:32","doi":"10.21203/rs.3.rs-2429792/v2","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2023-01-16 15:43:45","doi":"10.21203/rs.3.rs-2429792/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"fc496c1f-5d2f-4ad0-b1b4-2660215ae9b7","owner":[],"postedDate":"May 9th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":31545818,"name":"Physiology"}],"tags":[],"updatedAt":"2023-02-22T19:29:14+00:00","versionOfRecord":[],"versionCreatedAt":"2024-05-09 20:43:32","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v2","identity":"rs-2429792","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-2429792","identity":"rs-2429792","version":["v2"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}