The effect of IL-1 on the expression of proliferation factor of micromodules in interstitial cell of endometrial

【Objective】 To probe the effect of IL-1 on the dynamic equilibrium between VEGF and ENS and to study the paroxysm of endometioma at molecular level.【Method】 The interstitial cells were separated from endometrial and cultivated alone,and added different-potency IL-1 to the descents,collected different-period culture media,and to test the expression of IL-8,VEGF and ENS by means of ELISA.Meanwhile,immunohistochemistry tow-step procedure was used to test the cultivated cells and ascertain the purity of these cultivated cells.【Results】 In the case of endometioma,the sensibility of eutopic endometrial interstitial cells under the conditions of IL-1 increased;therefore high-tone IL-8 and VEGF came into being(P 0.01).Under the conditions of a certain-dense IL-1,the expression of IL-8 and VEGF increased with the time extension,which depended on time.The expressions of IL-8 and VEGF under the conditions of different-dense IL-1 depended on the dosage of IL-1.In the case of endometioma,the expression of ENS in the eutopic endometrial culture media,under the conditions of IL-1,had no obvious difference from the normal control group;moreover,the expression of ENS was not related to the change of time or dense of IL-1 at all.【Conclusion】 In the case of endometioma,the expression of IL-8 and VEGF ineutopic endometrial interstitial cells becomes strong under the conditions of IL-1;While the expression of ENS doesn't change obviously.It is the imbalance of angiogenesis.That leads to active growth of endometrial vascellum,which may be one paroxysm of the disease.