Sex Dependent Intergenerational Effects of Lead in Mouse Model

Sex Dependent Intergenerational Effects of Lead in Mouse Model | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Sex Dependent Intergenerational Effects of Lead in Mouse Model Nelly Banda, Nyein Soe, John Yabe, Rio Doya, Yared Yohannes, Yoshinori Ikenaka, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4573801/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 04 Dec, 2024 Read the published version in Scientific Reports → Version 1 posted 12 You are reading this latest preprint version Abstract Lead contaminated soils remains one of the major sources of lead (Pb) exposure with high impact on fertility in both males and females, pregnancy outcomes and child brain development. We investigated inhalation and ingestion as medium of Pb exposure via soil in male and female mice. Exposure was then withdrawn prior to mating thus we investigated the lingering effects of Pb on fertility in F0 generation and neurological development in F1 generation. Lead exposure reduced conception rates with a cumulative decrement being recorded when both males and females are exposed. Male testicular gene expression revealed reduction in fertility was caused by oxidative stress. Cessation of exposure did not alleviate neurological deficits in F1 generation, as offspring of exposed individuals performed poorly in the water Morris test when compared to control. The severity of the effects was also parent dependent and sex dependent. Paternal Pb exposure also caused some neurological and motor deficits, with effects being more pronounced in female offspring. The female offspring of paternal Pb exposure showed gene expression of changes occurring more in prefrontal cortex than in hippocampus, a trend recorded in their sires. This trend suggests that Pb neurological effects may be X- chromosome linked. Earth and environmental sciences/Environmental sciences Health sciences/Neurology Pb soil infertility neurological paternal maternal Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementarymaterialsforsubmission.BandaNellyetal.pdf Cite Share Download PDF Status: Published Journal Publication published 04 Dec, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 26 Sep, 2024 Reviews received at journal 26 Sep, 2024 Reviewers agreed at journal 06 Sep, 2024 Reviewers agreed at journal 25 Jul, 2024 Reviews received at journal 08 Jul, 2024 Reviewers agreed at journal 23 Jun, 2024 Reviewers agreed at journal 19 Jun, 2024 Reviewers invited by journal 19 Jun, 2024 Editor assigned by journal 19 Jun, 2024 Editor invited by journal 18 Jun, 2024 Submission checks completed at journal 14 Jun, 2024 First submitted to journal 13 Jun, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4573801","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":320583966,"identity":"ad444465-443b-4872-b367-25e2853ed24b","order_by":0,"name":"Nelly Banda","email":"","orcid":"","institution":"Hokkaido University","correspondingAuthor":false,"prefix":"","firstName":"Nelly","middleName":"","lastName":"Banda","suffix":""},{"id":320583968,"identity":"5db1be1d-2354-4aa7-8bc0-6d2670bcd262","order_by":1,"name":"Nyein Soe","email":"","orcid":"","institution":"Hokkaido 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