Effect of Photobiomodulation on Viability and Proliferation of Squamous Cell Carcinoma in Vitro

Effect of Photobiomodulation on Viability and Proliferation of Squamous Cell Carcinoma in Vitro | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Effect of Photobiomodulation on Viability and Proliferation of Squamous Cell Carcinoma in Vitro EMILLE DALBEM PAIM, Gisele Branchini, Marcelo Lamers, Leonardo Diel, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6636973/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 27 Oct, 2025 Read the published version in Lasers in Medical Science → Version 1 posted 12 You are reading this latest preprint version Abstract Head and neck cancer has a multifactorial etiology and significant cellular heterogeneity, which makes treatment difficult and contributes to high mortality rates. The need to combine different therapeutic modalities, such as surgery, chemotherapy, and radiotherapy, increases patient toxicity and requires a multidisciplinary approach to optimize results and improve quality of life. In recent years, photobiomodulation (PBM) has gained ground in tissue rehabilitation and regeneration. However, its effect on squamous cell carcinoma tumor cells lacks investigation. This study evaluated the effects of PBM on head and neck squamous cell carcinoma (SCC) based on cell proliferation and viability. We explored the behavior of two cell lines, SCC09 and CAL27, under 5 sequential applications of PBM using the Fluence HTM® device and laser pens of different wavelengths (830 nm and 658 nm) with irradiation doses of 1, 3, and 6 J/cm². To ensure standardization and avoid unwanted reflections, a specific adapter was developed, maintaining a fixed distance of 20 mm between the light source and the cells in culture, on a black surface. Cell viability was measured through the MTT ([3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide]) experiment and proliferation was evaluated through the Sulforhodamine B (SR-B) assay. No significant effects on cell proliferation or viability of the evaluated cell lines were identified. PBM did not demonstrate a significant effect on cell proliferation and viability under the experimental conditions tested. However, the variability found in other studies highlights the need for additional research to elucidate the mechanisms of action of PBM and establish safe treatment protocols. Photobiomodulation Therapy Cell Atypical Squamous Head and Neck Neoplasms Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 27 Oct, 2025 Read the published version in Lasers in Medical Science → Version 1 posted Editorial decision: Revision requested 18 Aug, 2025 Reviews received at journal 12 Aug, 2025 Reviews received at journal 09 Aug, 2025 Reviewers agreed at journal 30 Jul, 2025 Reviewers agreed at journal 28 Jul, 2025 Reviews received at journal 28 Jul, 2025 Reviewers agreed at journal 28 Jul, 2025 Reviewers agreed at journal 25 Jul, 2025 Reviewers invited by journal 10 Jun, 2025 Editor assigned by journal 10 Jun, 2025 Submission checks completed at journal 27 May, 2025 First submitted to journal 10 May, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6636973","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":469370906,"identity":"eecab6ab-3d2c-4b74-ba5e-6f43fff26692","order_by":0,"name":"EMILLE DALBEM 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