In vivo18F-DOPA PET imaging identifies a dopaminergic deficit in a rat model with a G51D α-synuclein mutation

Parkinson’s disease (PD) is a neurodegenerative condition with several major hallmarks, including loss of substantia nigra neurons, reduction in striatal dopaminergic function, and formation of α-synuclein-rich Lewy bodies. Mutations in SNCA , encoding for α-synuclein, are a known cause of familial PD, and the G51D mutation causes a particularly aggressive form of the condition. CRISPR/Cas9 technology was used to introduce the G51D mutation into the endogenous rat SNCA gene. SNCA G51D/+ and SNCA G51D/G51D rats were born in Mendelian ratios and did not exhibit any severe behavourial defects. L -3,4-dihydroxy-6- 18 F-fluorophenylalanine ( 18 F-DOPA) positron emission tomography (PET) imaging was used to investigate this novel rat model. Wild-type (WT), SNCA G51D/+ and SNCA G51D/G51D rats were characterised over the course of ageing (5, 11, and 16 months old) using 18 F-DOPA PET imaging and kinetic modelling. We measured the influx rate constant ( K i ) and effective distribution volume ratio ( EDVR ) of 18 F-DOPA in the striatum relative to the cerebellum in WT, SNCA G51D/+ and SNCA G51D/G51D rats. A significant reduction in EDVR was observed in SNCA G51D/G51D rats at 16 months of age indicative of increased dopamine turnover. Furthermore, we observed a significant asymmetry in EDVR between the left and right striatum in aged SNCA G51D/G51D rats. The increased and asymmetric dopamine turnover observed in the striatum of aged SNCA G51D/G51D rats is similar to prodromal PD, which suggests the presence of compensatory mechanisms. SNCA G51D rats represent a novel genetic model of PD, and kinetic modelling of 18 F-DOPA PET data has identified a highly relevant early disease phenotype.