Expressions of NF-κB and MIF in endometrial polyps among women of reproductive age

Objective:To explore the relationship between nuclear factor-kappa B(NF-κB),macrophage migration inhibitory factor(MIF) and occurrence of endometrial polyps among women of reproductive age and the correlation between NF-κB expression and MIF expression in endometrial polyps.Methods:The samples of endometrial polyps and surrounding endometrial tissue of 42 patients with endometrial polyps and normal endometrial samples of 22 patients with uterus septum were collected after hysteroscopic surgery,RT-PCR technique was used to detect the expressions of NF-κBp65 and MIF mRNA;immunohistochemical SP method was used to detect the expressions of NF-κBp65 and MIF protein.Results:The expression levels of NF-κBp65 and MIF mRNA in endometrial polyps were statistically significantly higher than those in surrounding endometrial tissue and normal endometrium during the same period(P0.05);compared with normal endometrium,the expression levels of NF-κBp65 and MIF mRNA in surrounding endometrial tissue increased significantly(P0.05).NF-κBp65 and MIF protein expressed in the three groups,which were the highest in endometrial polyps group,followed by surrounding endometrial tissue,there were statistically significant difference in the expression levels of NF-κBp65 and MIF protein among the three groups(P0.05).There was a positive correlation between NF-κBp65 expression and MIF mRNA expression in endometrial polyps(γ=0.694,P0.05),and NF-κBp65 was positively correlated with MIF protein in endometrial polyps(γ=0.37,P0.05).Conclusion:NF-κB and MIF may be correlated with pathogenesis of endometrial polyps in women of reproductive age,which play synergistic roles in pathological change of occurrence and development of endometrial polyps.