Targeted Glioblastoma Eradication via CRISPR-Modified Macrophages

Targeted Glioblastoma Eradication via CRISPR-Modified Macrophages | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Targeted Glioblastoma Eradication via CRISPR-Modified Macrophages Diggaj Jain This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6297341/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Glioblastoma remains an incurable and highly aggressive brain tumor due to its immune-suppressive microenvironment. In this paper, we propose a novel theoretical approach using CRISPR-engineered macrophages to infiltrate, recognize, and eradicate glioblastoma. Mathematical and Theoretical Biology Cancer Biology Biotechnology and Bioengineering Glioblastoma CRISPR Macrophages Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6297341","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":433322534,"identity":"f764b339-c3bd-45d0-acd3-9980fca2b8eb","order_by":0,"name":"Diggaj Jain","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA/ElEQVRIiWNgGAWjYDCCwyCCh42BDUgxM1SASOYGUrScAZGMBLQcQGIzM7aBKAJa+I6zX37xQ4ZPnk+695h04bzaaP52oJYfFdtwapE8zFNm2cPDZtgmcy5Neua247kzDjM2MPacuY1Ti8FhnjQDHh42xjaJHDNp3m3HchuAWoAuxK/F8A8Pmz1Ey5xjufMJa2E//BhoSyJES0NN7gZCWoB+YWOW4WFLbpPIS7aecexA7kagloP4/MJ3/vjjj297jtnOn5F78HZBTV3uvPOHDz74UYFbCzAazSQYe46BGCAeOGZRIgsLYH/8geFHDUxLHX7Fo2AUjIJRMCIBABMXWQfTgxpBAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0009-0000-3888-3070","institution":"Bright Day School, Vadodara, India","correspondingAuthor":true,"prefix":"","firstName":"Diggaj","middleName":"","lastName":"Jain","suffix":""}],"badges":[],"createdAt":"2025-03-24 16:43:51","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-6297341/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6297341/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":79143846,"identity":"85b54499-404d-40a8-8a70-779e6ecb2236","added_by":"auto","created_at":"2025-03-25 02:35:21","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":184864,"visible":true,"origin":"","legend":"","description":"","filename":"Cancer.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6297341/v1_covered_147eeb9f-bfb8-4c3d-a61c-e9afeb077265.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003eTargeted Glioblastoma Eradication via CRISPR-Modified Macrophages\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Bright Day School","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Glioblastoma, CRISPR, Macrophages","lastPublishedDoi":"10.21203/rs.3.rs-6297341/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6297341/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eGlioblastoma remains an incurable and highly aggressive brain tumor due to its immune-suppressive microenvironment. In this paper, we propose a novel theoretical approach using CRISPR-engineered macrophages to infiltrate, recognize, and eradicate glioblastoma.\u003c/p\u003e","manuscriptTitle":"Targeted Glioblastoma Eradication via CRISPR-Modified Macrophages","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-03-25 02:27:15","doi":"10.21203/rs.3.rs-6297341/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"bf6dd2a9-8569-4ca2-ba45-7f28c2297551","owner":[],"postedDate":"March 25th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":46140830,"name":"Mathematical and Theoretical Biology"},{"id":46140831,"name":"Cancer Biology"},{"id":46140832,"name":"Biotechnology and Bioengineering"}],"tags":[],"updatedAt":"2025-03-25T02:27:15+00:00","versionOfRecord":[],"versionCreatedAt":"2025-03-25 02:27:15","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6297341","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6297341","identity":"rs-6297341","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}