Microbial hydrogen consumption leads to a significant pH increase under high-saline-conditions– implications for hydrogen storage in salt caverns

Salt caverns have been successfully used for natural gas storage globally since the 1940s and are now under consideration for hydrogen (H 2 ) storage, which is needed in large quantities for the Green Shift. Salt caverns are not sterile, and H 2 is a ubiquitous electron donor for microorganisms. This could entail that the injected H 2 will be microbially consumed, leading to a volumetric loss and potential production of toxic H 2 S. However, the extent and rates of this microbial H 2 consumption under high-saline cavern conditions are not yet understood. To investigate microbial consumption rates, we cultured the halophilic sulphate-reducing bacteria Desulfohalobium retbaense and the halophilic methanogen Methanocalcus halotolerans under different H 2 partial pressures. Both strains consumed H 2 , but consumption rates slowed down significantly over time. The activity loss correlated with a significant pH increase (up to pH 9) in the media due to intense proton- and bicarbonate consumption. In the case of sulphate-reduction, this pH increase led to dissolution of all produced H 2 S in the liquid phase. We compared these observations to an original brine retrieved from a salt cavern located in Northern Germany, which was incubated with 100% H 2 over several months. We again observed a H 2 loss (up to 12%) with a concurrent increase in pH up to 8.5 especially when additional nutrients were added to the brine. Our results clearly show that sulphate-reducing microbes present in salt caverns will consume H 2 , which will be accompanied by a significant pH increase, resulting in reduced activity over time. This potentially self-limiting process of pH increase during sulphate-reduction will be advantageous for H 2 storage in low-buffering environments like salt caverns.