Magnetic-assisted manipulation of rare blood cells for diagnosis: A systematic review

Magnetic-assisted manipulation of rare blood cells for diagnosis: A systematic review | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Biotechnology and Bioengineering This is a preprint and has not been peer reviewed. Data may be preliminary. 24 March 2025 V1 Latest version Share on Magnetic-assisted manipulation of rare blood cells for diagnosis: A systematic review Authors : Poornima Iyer 0009-0009-0527-6202 , Xian Wu , Hyeon Choe , Linh Nguyen T. Tran , Karla Mercedes Paz González , Bahareh Rezaei , Shahriar Mostufa , Ebrahim Azizi , Ioannis H. Karampelas , Kai Wu , Jeffrey Chalmers 0000-0003-1723-9774 , and Jenifer Gomez-Pastora [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174285560.06832046/v1 Published Biotechnology and Bioengineering Version of record Peer review timeline 393 views 220 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The precise isolation and analysis of rare cells from blood are crucial for biomedical research and clinical diagnostics. This review examines recent advancements in magnetic-based separation techniques, focusing on their efficiency in capturing rare cells such as circulating tumor cells (CTCs), circulating fetal cells, and diseased red blood cells (RBCs). These methods use magnetophoresis under external magnetic fields for highly specific isolation with minimal contamination, offering advantages over traditional techniques in speed, cost-effectiveness, and robustness. Magnetic separation is categorized into label-based methods, which use immunomagnetic nanoparticles (IMNs) to target specific cell markers, and label-free methods, which exploit differences in magnetic susceptibility. Both approaches have achieved up to 99% efficiency in isolating diseased RBCs and CTCs. However, challenges remain in improving purity, scalability, and clinical applicability. A key limitation of label-based methods is the need to detach cells from magnetic beads without compromising viability. Label-free technologies, such as magnetic levitation, enable ligand-free separation based on density and susceptibility. Future research should focus on optimizing paramagnetic media, integrating machine learning for enhanced accuracy, and developing high-gradient magnetic fields (~1,000 T/m) to improve efficiency. Advancements in IMNs with stronger magnetic properties will further enhance separation performance, driving clinical translation. Supplementary Material File (magnetic_separation_of_rare_blood_cells_for_diagnosis_biotechnologyandbioengineering.docx) Download 3.73 MB Information & Authors Information Version history V1 Version 1 24 March 2025 Peer review timeline Published Biotechnology and Bioengineering Version of Record 26 Jun 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Biotechnology and Bioengineering Keywords ctcs fetal cells infected or anemic rbcs label-free labelled magnetic-based separation Authors Affiliations Poornima Iyer 0009-0009-0527-6202 The Ohio State University William G Lowrie Department of Chemical and Biomolecular Engineering View all articles by this author Xian Wu The Ohio State University William G Lowrie Department of Chemical and Biomolecular Engineering View all articles by this author Hyeon Choe The Ohio State University William G Lowrie Department of Chemical and Biomolecular Engineering View all articles by this author Linh Nguyen T. Tran Texas Tech University Department of Chemical Engineering View all articles by this author Karla Mercedes Paz González Texas Tech University Department of Chemical Engineering View all articles by this author Bahareh Rezaei Texas Tech University View all articles by this author Shahriar Mostufa Texas Tech University View all articles by this author Ebrahim Azizi Texas Tech University View all articles by this author Ioannis H. Karampelas Nemak USA Inc View all articles by this author Kai Wu Texas Tech University View all articles by this author Jeffrey Chalmers 0000-0003-1723-9774 The Ohio State University William G Lowrie Department of Chemical and Biomolecular Engineering View all articles by this author Jenifer Gomez-Pastora [email protected] Texas Tech University Department of Chemical Engineering View all articles by this author Metrics & Citations Metrics Article Usage 393 views 220 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Poornima Iyer, Xian Wu, Hyeon Choe, et al. Magnetic-assisted manipulation of rare blood cells for diagnosis: A systematic review. Authorea . 24 March 2025. DOI: https://doi.org/10.22541/au.174285560.06832046/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. 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