The Value of PET/CT Radiomics for Predicting Survival Outcomes in Patients with Pancreatic Ductal Adenocarcinoma

The Value of PET/CT Radiomics for Predicting Survival Outcomes in Patients with Pancreatic Ductal Adenocarcinoma | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article The Value of PET/CT Radiomics for Predicting Survival Outcomes in Patients with Pancreatic Ductal Adenocarcinoma Yeon-koo Kang, Seunggyun Ha, Ji Bong Jeong, So Won Oh This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4825555/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 22 Nov, 2024 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Pancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis even without distant metastases, necessitating in-depth characterization of primary tumours for survival prediction. We assessed the feasibility of using FDG-PET/CT radiomics to predict overall survival (OS) in PDAC. This retrospective study included PDAC patients without distant metastasis who underwent FDG-PET/CT for initial staging. Primary tumours were segmented from FDG-PET/CT images, extracting 222 radiomics features. A radiomics-based risk score (Rad-score) was developed using Cox proportional hazards regression with LASSO to predict OS. The prognostic performance of the Rad-score was compared with a clinical model (demographics, disease stage, laboratory results) using Harrell's concordance index (C-index) and bootstrapping. 140 patients were included, with a mortality rate was 72.9% during follow-up (total population, 19.5 ± 19.2 months; survivors, 34.4 ± 28.8 months). Eleven radiomics features were significant for survival prediction. The Rad-score predicted OS with a C-index of 0.681 [95% CI, 0.632–0.731]. A model integrating clinical parameters and Rad-score outperformed the clinical-only model in predicting OS (C-index 0.736 [0.727–0.812] vs. 0.667 [0.648–0.750]; C-index difference 0.069 [0.028–0.117]; P < 0.001). These findings suggest that incorporating FDG-PET/CT radiomics into preexisting prognotic stratification paradiagm may enhance survival prediction in PDAC, warranting large-scale studies to confirm its applicability in clinical practice. Health sciences/Oncology/Cancer/Gastrointestinal cancer/Pancreatic cancer Health sciences/Oncology/Cancer/Cancer imaging Health sciences/Biomarkers/Prognostic markers FDG-PET/CT Pancreas cancer Radiomics Survival Figures Figure 1 Figure 2 Introduction Pancreatic ductal adenocarcinoma (PDAC) is often associated with a poor prognosis due to its aggressive nature and resistance to conventional therapies 1 . Nearly half of newly diagnosed patients with PDAC present with distant metastases at the time of primary tumour detection due to early dissemination and high risk of metastasis 2 . PDAC tumours often exhibit significant heterogeneity, rendering them refractory to standard chemotherapy and targeted interventions. Despite advancements in surgical techniques, radiation, and chemotherapy, the 5-year survival rate for PDAC remains remarkably low, typically ranging between 10% and 15% 3 . Survival outcomes vary contingent on factors such as the stage of cancer at diagnosis and individual patient characteristics. Consequently, it is important to identify specific tumour characteristics associated with poor prognosis. Positron Emission Tomography (PET) using F-18 fluorodeoxyglucose (FDG) is a valuable tool in oncology, providing functional imaging data which is critical for assessing tumour characteristics. PET-derived metrics encompassing key parameters such as maximum standardised uptake value, metabolic tumour volume, and total lesion glycolysis, are instrumental in prognosticating therapeutic response. Nonetheless, these parameters may not comprehensively capture the intricacies of spatial tumour complexity which is intrinsically linked with cellular and molecular dynamics, including proliferation and necrosis 4 , 5 . Intratumoural heterogeneity (ITH) may have prognostic clinical importance, particularly in appraising resistance to therapeutic interventions. Various image features, such as texture analysis, have been proposed for the evaluation of ITH 6 . Therefore, there is a need to quantitatively discern image patterns that have clinical relevance, particularly within the domain of spatial tumoural complexity, in order to discover and develop valuable clinical indicators and models for precise prognostication. Radiomics analysis has recently emerged as a sophisticated, integrative, data-driven approach for quantifying complex image patterns and constructing clinically valuable models using imaging data. Extending beyond the limits of visual interpretation and basic quantitative assessment 7 , this approach employs automated, high-throughput extraction and refinement techniques to analyse a vast spectrum of image patterns from medical images. FDG- PET radiomics represents a noteworthy advancement in the quantitative analysis of imaging data and has attracted attention for its potential in assessing spatial tumoural complexity, including ITH, and predicting therapeutic response in the field of oncology. In this study, we aimed to develop and validate a model based on radiomics analysis of FDG-PET/computed tomography (CT) images of primary tumours to predict the overall survival (OS) of patients newly diagnosed with PDAC. Methods Patients and Overall Study Design This study received institutional review board approval from Seoul Metropolitan Government - Seoul National University, Boramae Medical Center (SMG-SNU BMC; approval number: 30-2022-72), and was performed in accordance with the Declaration of Helsinki. The board granted a waiver of informed consent for this study due to its retrospective design, recognising its minimal risk to participants. This retrospective, single-centre, medical record-review study was designed to predict the therapeutic response of patients who underwent FDG-PET/CT for initial staging of PDAC between June 2010 and October 2020. Patients were included if they met the following criteria: (a) the presence of FDG-avid primary tumour; (b) the absence of clinically suspected distant metastases based on CT, MRI, and FDG-PET/CT images; (c) the absence of any other primary malignancy; and (d) no history of receiving any anti-tumour treatment prior to PET/CT imaging, including surgery, chemotherapy, or radiotherapy. Patients were excluded if they had at least one of the following criteria: (a) tumour size too small to evaluate FDG distribution (< 64 voxels); (b) PET/CT misregistration due to respiratory motion; and (c) limited delineation of tumour uptake on FDG-PET/CT images due to adjacent activity of the stomach or inflamed pancreas. A flow chart depicting patient selection is shown in Fig. 1 . Prognostic models were constructed using both clinical parameters and FDG-PET/CT radiomics analysis, which were then validated to predict OS. The commencement of observation was defined as the first date of referral to SNM-SNU BMC for the management of pancreatic cancer. Each patient’s survival data was obtained from the national database of the Ministry of the Interior and Safety of South Korea, with December 2020 as the cut-off date. Demographic and clinical characteristic data were extracted from the medical records of each patient from the point of initial PDAC diagnosis. FDG-PET/CT Imaging Fasting status was confirmed by blood glucose levels (< 140 mg/dL). Subsequently, an intravenous injection of fluorodeoxyglucose (FDG) was administered at a dose of 5.18 MBq/kg. After a 60-minute rest period, a non-contrast CT scan was conducted using parameters of 80 mA and 140 kVp with a slice thickness of 5 mm. This was immediately followed by 1 minute of PET imaging per bed position utilising a dedicated PET/CT scanner (Gemini TF, Philips Healthcare, Cleveland, OH). CT images were reconstructed using a 512 × 512 matrix across a 50 cm field-of-view. PET scans covered the area extending from the mid-thigh to the vertex and were reconstructed using a 128 × 128 matrix. PET images were processed using the ordered subset expectation maximisation algorithm, with parameters of n subsets and m iterations, enhanced with time-of-flight and point spread function. Additionally, CT-based attenuation correction and application of a Gaussian filter with a full width at half maximum of 8 mm were employed to optimise image clarity and diagnostic accuracy. Feature Extraction from FDG-PET/CT A nuclear medicine physician (Y.K.K) with 10 years of experience performed PET/CT interpretation analysed PET/CT images using free medical image texture analysis software (LIFEx 7.4.0, http://www.lifexsoft.org ) 8 . For segmentation of pancreatic tumours, spherical volumes of interest were manually delineated on PET images encompassing the entirety of each tumour, while a contrast-based approach was used to define tumour contours. Normal retroperitoneal fat tissue was excluded from the defined tumour volume via visual assessment of corresponding CT images. Texture parameters were automatically calculated from the final segmented tumour volume derived from PET and CT images. Intensity discretisation was carried out using a fixed bin size method for both PET (bin size, 0.5 standardized uptake value; range, 0–50) and CT (bin size, 10 Hounsfield unit; range, -150–150) images. A total of 222 texture parameters were used as input factors of the radiomics model. The extracted features were grouped under the following heading: including morphology, intensity, local intensity, intensity histogram, grey level co-occurrence, grey level run length, grey level size zone, and neighbourhood grey level difference features (Table S1 ). Development of a Radiomics Model A radiomics model was developed to predict OS. To identify parameters with significant prognostic influence among the extracted texture features, we employed the least absolute shrinkage and selection operator (LASSO) method for variable selection and shrinkage within the Cox proportional hazards regression analysis. We conducted 10-fold cross-validation on the entire patient cohort to minimize overfitting and optimise model hyperparameters. The model was optimised to maximise the Harrell’s concordance index (C-index). A radiomics score (Rad-score) was then produced for each patient by summing the selected prognostic variables weighted by their coefficients obtained from the LASSO Cox regression analysis. The Rad-score was used for the subsequent survival analysis. Statistical Analysis Clinical characteristics were compared between patients who survived and those who did not using Student’s t-test for continuous variables and chi-square test for categorical variables. The prognostic significance of clinical variables and the Rad-score for OS were assessed by univariate and multivariate Cox proportional hazards regression analyses, respectively. Harrell's C-index was used to represent the prognostic values of Cox models based on clinical variables (clinical model), Rad-score (Rad-score model), and a combination of both factors (clinical + Rad-score model). The difference in Harrell's C-index between the clinical model and the clinical + Rad-score model was assessed using bootstrapping with 1,000 resamples to determine the additive value of the Rad-score. Radiomics model development and statistical analyses were performed using R software 4.3.3 ( http://www.R-project.org ). LASSO Cox regression analyses were performed using the "glmnet" package embedded in R software ( https://cran.r-project.org/web/packages/glmnet ). A two-sided P-value below 0.05 was considered statistically significant. In the multivariate Cox analyses, variables were included in the model if their p-value was less than 0.05 and were removed if their p-value exceeded 0.1. Results Patient Demographics and Clinical Characteristics The study included 84 male and 56 female patients diagnosed with PDAC, with an average age of 69.0 ± 10.6 years. The clinical staging distribution was as follows: T1 stage, 10.7% (n = 15); T2, 27.9% (n = 39); T3, 25.0% (n = 35); and T4, 36.5% (n = 51). A considerable majority (75.0%) of patients had no suspected regional lymph node metastases. Most patients (78.6%; n = 110) received initial treatment at SNM-SNU BMC, including upfront surgery (36.36%; n = 40), systemic chemotherapy (30.91%; n = 34), and best supportive care alone (32.73%; n = 36). The Ministry of the Interior and Safety of South Korea’s national statistical database indicated that, among the included patients, 102 patients (72.8%) were deceased at the cut-off timepoint, with an average follow-up duration of 19.5 ± 19.2 months for the entire cohort, and 34.4 ± 28.8 months for survivors. No significant differences in demographic or clinical characteristics, including age, sex, smoking history, and clinical staging, were noted between survivors and non-survivors ( Table 1 ). Table 1 Clinical characteristics of patients. Factor Survived (N = 38) Deceased (N = 102) Total (N = 140) P * Age (year) 67.3 ± 9.4 69.7 ± 11.0 69.0 ± 10.6 0.241 Sex (Male: Female) 22:16 62:40 84:56 0.847 Follow-up periods (months) 34.4 ± 28.8 14.0 ± 9.4 19.5 ± 19.2 Smoking history Smoker or ex-smoker 5 (13.2%) 25 (24.5%) 30 0.147 Never-smoker 33 (86.8%) 77 (75.5%) 110 Clinical T stage T1 4 (10.5%) 11 (10.8%) 15 (10.7%) 0.133 T2 16 (42.1%) 23 (22.5%) 39 (27.9%) T3 8 (21.1%) 27 (26.5%) 35 (25.0%) T4 10 (26.3%) 41 (40.2%) 51 (36.5%) Clinical N stage N0 26 (68.4%) 79 (77.5%) 105 (75.0%) 0.158 N1 11 (28.9%) 16 (15.7%) 27 (19.3%) N2 1 (2.6%) 7 (6.9%) 8 (5.7%) Haemoglobin (g/dL) 13.1 ± 1.8 12.5 ± 2.0 12.7 ± 1.9 0.096 WBC (10 9 /L) 7.1 ± 3.0 7.2 ± 2.9 7.2 ± 2.9 0.786 Platelet (10 3 /µL) 243.2 ± 62.5 260.0 ± 85.0 255.2 ± 79.6 0.277 AST (IU/L) 148.5 ± 224.8 122.8 ± 149.3 129.7 ± 172.6 0.435 ALT (IU/L) 166.2 ± 202.9 134.4 ± 171.2 143.1 ± 180.5 0.357 ALP (IU/L) 303.9 ± 224.6 388.9 ± 387.9 365.9 ± 352.5 0.111 Total bilirubin (mg/dL) 8.7 ± 9.4 7.5 ± 9.5 7.8 ± 9.5 0.491 CEA (ng/mL) 3.6 ± 4.5 8.5 ± 23.6 7.1 ± 20.2 0.052 CA19-9 (U/mL) 2,085.8 ± 7,151.4 2,427.6 ± 12,330.4 2,333.5 ± 11,122.2 0.873 *Student’s t tests for continuous parameters and chi square tests for categorical parameters were performed. WBC, white blood cell; AST, aspartate transaminase; ALT, alanine transferase; ALP, alkaline phosphatase; CEA, carcinoembryonic antigen; CA19-9, carbohydrate antigen 19 − 9 Radiomics Model Development LASSO Cox proportional hazards regression model assisted with optimal hyperparameters selected seven features of PET images and four features of CT images from among the 222 texture features extracted from PET/CT images (Figure S1 in Supplementary data) to construct the radiomics model. Details of the selected features and Rad-score calculation are as follows: where MORPH_AV represents Surface To Volume Ratio, IH_SKEW represents Intensity Histogram Skewness, IH_P10 represents Intensity Histogram 10th percentile, IH_energy represents Intensity Histogram Energy, GLCM_NIDM represents GLCM Normalized Inverse Difference Moment, NGTDM_STR represents NGTDM strength, GLSZM_LZLGE represents GLSZM Large Zone Low Grey Level Emphasis, SKEW represents Intensity-based Skewness, MIN represents Minimum Grey Level, GLRLM_LRLGE represents GLRLM Long Run Low Grey Level Emphasis, and GLSZM_GLNU_NORM represents GLSZM Normalized Grey Level Non-uniformity. ' PET_ ' and ' CT_ ' prefixes indicate factors derived from PET and CT images, respectively. The composite Rad-score demonstrated significant prognostic value with a C-index of 0.671. Prognostic Significance of Clinical Variables and Radiomics Score Univariate Cox proportional hazards regression analysis to assess the prognostic value of clinical parameters and the Rad-score (Table 2 ) indicated that significant predictors of OS in patients with PDAC were age (HR, 1.040; 95% CI, 1.016–1.064; P = 0.0027), serum haemoglobin (Hb) level (HR, 0.840; 95% CI, 0.750–0.94; P < 0.0001), alkaline phosphatase (ALP) level (HR, 1. 001; 95% CI, 1.000–1.001; P = 0.0139), and carcinoembryonic antigen (CEA) level (HR, 1.021; 95% CI, 1.012–1.031; P < 0.0001). Smoking history showed borderline significance (HR, 1.510; 95% CI, 0.942–2.420; P = 0.0869) in predicting survival. T stage (4 vs. <4), N stage, and other laboratory test results had no significant prognostic impact. Notably, the Rad-score outperformed all clinical parameters with a higher hazard ratio (HR,12.698; 95% CI, 5.743–28.074; P < 0.0001). In Kaplan-Meier curve analysis with log-rank test using an optimal cutoff that produced the highest chi-square of Log-rank test, patients with higher and lower Rad-score demonstrated significant differences in overall survival (median OS 10.8 [95% CI 8.4–14.2] vs. 27.1 [19.6–34.1] months; chi-square = 29.254; P < 0.001; Fig. 2 ). Table 2 Univariate survival analysis using clinical parameters and radiomics score. N+, presence of regional lymph node metastasis; WBC, white blood cell; AST, aspartate transaminase; ALT, alanine transferase; ALP, alkaline phosphatase; CEA, carcinoembryonic antigen; CA19-9, carbohydrate antigen 19 − 9 Factor Hazard ratio [95% CI] P Age 1.040 [1.016–1.064] 0.001 Sex 1.022 [0.678–1.541] 0.918 Smoking history 1.510 [0.942–2.420] 0.087 Alcohol intake 1.021 [0.673–1.549] 0.922 T4 (vs. T1-3) 1.283 [0.850–1.937] 0.235 N+ 0.818 [0.508–1.319] 0.411 WBC 1.070 [0.991–1.154] 0.082 Haemoglobin 0.840 [0.750–0.941] 0.003 Platelet 1.002 [0.999–1.004] 0.207 AST 1.000 [0.999–1.001] 0.551 ALT 1.000 [0.999–1.001] 0.774 ALP 1.001 [1.000–1.001] 0.014 Total bilirubin 1.010 [0.988–1.033] 0.369 CEA 1.021 [1.012–1.031] < 0.001 CA19-9 1.000 [1.000–1.000] 0.175 Rad-score 12.698 [5.743–28.074] < 0.001 N+, presence of regional lymph node metastasis; WBC, white blood cell; AST, aspartate transaminase; ALT, alanine transferase; ALP, alkaline phosphatase; CEA, carcinoembryonic antigen; CA19-9, carbohydrate antigen 19-9 Additive Value of the Radiomics Score to Clinical Prognostics Multivariate Cox proportional hazards regression analysis compared the prognostic value of clinical and combined (clinical + Rad-score) survival prediction models (Table 3 ). In the clinical model, age, smoking history, serum ALP level, CEA level, and T stage were identified as independent predictors of OS. In the clinical + Rad-score model, Rad-score was as a significant prognostic factor (HR, 12.793; 95% CI, 5.54–29.546; P < 0.0001). Thus, Rad-score was included in the model and T stage was excluded. Inclusion of Rad-score in the clinical model improved prognostic accuracy as evidenced by a higher Harrell's C-index (0.740 vs. 0.673). Bootstrapping analysis showed that the difference in prognostic value between the clinical and clinical + Rad-score models was statistically significant (difference in C-index, 0.069; 95% CI, 0.014–0.113; P < 0.0001). Table 3 Multivariate survival analysis using clinical parameters and radiomics score. Factor Clinical model Combined model Hazard ratio [95% CI] P Hazard ratio [95% CI] P Age 1.050 [1.025–1.077] < 0.001 1.052 [1.026–1.078] < 0.001 Smoking history 2.067 [1.242–3.440] 0.005 2.080 [1.237–3.498] 0.006 T4 (vs. T1-3) 1.454 [0.940–2.251] 0.093 N/S N+ N/S N/S Haemoglobin N/S N/S ALP 1.001 [1.000–1.001] 0.043 1.001 [1.000–1.001] 0.034 CEA 1.021 [1.011–1.031] < 0.001 1.014 [1.004–1.025] 0.007 CA19-9 N/S N/S Rad-score - - 12.793 [5.540–29.546] < 0.001 C-index 0.673 [0.650–0.766] 0.740 [0.715–0.816] C-index difference* 0.067 [0.014–0.113] *Difference between two models produced by bootstrapping 1,000 resamples. N/S, not significant; ALP, alkaline phosphatase; CEA, carcinoembryonic antigen; CA19-9, carbohydrate antigen 19 − 9 Discussion We successfully formulated a prognostic model for the evaluation of patients with PDAC using FDG-PET/CT-based radiomics. By identifying key features through texture analysis of FDG-PET/CT images and integrating them into a composite Rad-score, we constructed a model with a nuanced approach to oncologic prognostication. The Rad-score was validated, demonstrating statistically significant, albeit moderate, prognostic ability to discriminate between survivors and decedents, indicating its potential utility in clinical prognostication. A combined model, incorporating both clinical parameters and Rad-score significantly outperformed the use of clinical parameters alone for predicting OS in patients with PDAC, indicating that Rad-score may be a crucial biomarker for survival outcomes. In addition, multivariate Cox proportional hazards regression analysis demonstrated that integrating the Rad-score with clinical variables significantly enhanced the prognostic accuracy of OS prediction models. This study underscores the critical contribution of radiomics in refining the accuracy of survival prediction and its potential in guiding personalised treatment in oncology. Several studies have demonstrated the prognostic value of radiomics in pancreatic cancer, mainly based on contrast-enhanced CT or MRI 9 . However, research focusing on FDG PET-based radiomics for predicting OS in pancreatic cancer remains limited. Toyama et al. showed the prognostic value of GLZLM grey-level non-uniformity in FDG-PET using a random forest classifier, but this study only estimated survival outcomes over a one-year observation period 10 . Hyun et al. analysed a cohort of 137 PDAC patients and reported that first-order entropy on initial FDG-PET independently correlates with survival, as determined through multivariate Cox regression analysis 11 . Similarly, JW Lee et al. demonstrated the prognostic significance of first-order entropy and developed and reported a scoring system that incorporates total lesion glycolysis and bone marrow uptake to predict OS 12 . Conversely, Yoo et al., in a study of patients with pancreatic cancer undergoing curative surgery, failed to identify independent predictive factors from heterogeneous features measured on FDG-PET through multivariate analysis 13 . Despite providing us with valuable insights, the previous studies are somewhat limited by their focus on individual radiomics features. These studies used conventional Cox regression analysis, which has inherent shortcomings such multicollinearity, ineffective variable selection, and potential overfitting. Addressing these issues, we employed LASSO for robust variable selection and designed an integrated prognosis model to emphasize the power of integrated radiomics data. Nonetheless, we highlight the need for ongoing optimisation to improve the predictive performance of the model. Additionally, we investigated the additive value of radiomics data by comparing it with a prognosis model based on clinical variables alone, further validating our approach through internal bootstrapping analysis. In our study, the Rad-score emerged as an independent prognostic indicator in the multivariate analysis, while T stage did not retain its prognostic significance within the radiomics-enhanced model (clinical + Rad-score model). This change suggests the potential of the radiomics model to serve as a more comprehensive and robust prognostic tool by capturing the intricate characteristics of primary tumors, which may not be fully appreciated through traditional clinical markers alone. Additionally, while certain clinical prognostic factors such as age, smoking history, and carcinoembryonic antigen (CEA) levels aligned with findings from previous studies, other factors like N stage and carbohydrate antigen 19 − 9 (CA19-9) did not 14 – 18 . This discrepancy may be attributed to the heterogeneous disease status and the variability in treatment and follow-up strategies among patients involved in the study. The need for further validation in a large multi-center cohort with unified management strategies remains to solidify its usefullness in clinical practice. This study has several limitations. First, its retrospective design has an inherent risk of bias because it includes patients who received different treatment regimens, several of whom received follow-up treatment after initial diagnosis at hospitals other than SNM-SNU BMC. However, given the strict regulation of cancer treatment by the Korean national insurance system, it is plausible that most patients received standardised treatment in accordance with national cancer treatment guidelines (references). Second, while this study included a larger number of patients compared with previous studies, its single-centre design may limit the generalizability of the results. Prospective multi-centre studies with external data are warranted. Third, lesions were excluded from analysis if there was insufficient tumour size for evaluating FDG distribution, PET-CT misregistration due to respiratory motion, or challenges in delineating tumour uptake on FDG-PET/CT images. This exclusion criteria are a common challenge in PET radiomics analyses, because it safeguards appropriate texture analysis 6 . An FDG-PET/CT-based radiomics model showed potential in enhancing the prediction of survival outcomes among patients with PDAC, and outperformed a model based on clinical data alone demonstrating its potential applicability in the field. Further prospective studies with larger cohorts are warranted to validate the results of the current study and establish the model’s applicability in patient management. Declarations Acknowledgements The authors declare no conflict of interest. Competing Interests Statement The authors declare no competing interests. Author Contribution Y.K., S.H., J.B.J., and S.W.O. designed and conceptualized the study. Y.K. and S.H. collected data, and performed radiomics analysis and statistical analysis. J.B.J. and S.W.O. critically discussed the analysis and results. Y.K. and S.H. initially wrote the manuscript. J.B.J. and S.W.O. revised the manuscript. All these authors read and approved the final version of the manuscript. Data Availability The datasets generated or analyzed during the study are available from the corresponding author on reasonable request. Due to patient confidentiality agreements, raw imaging data are not publicly available but can be accessed upon approval by the institutional review board. References Rawla, P., Sunkara, T. & Gaduputi, V. Epidemiology of Pancreatic Cancer: Global Trends, Etiology and Risk Factors. World J Oncol 10, 10–27, doi: 10.14740/wjon1166 (2019). De Dosso, S. et al. Treatment landscape of metastatic pancreatic cancer. Cancer Treat Rev 96, 102180, doi: 10.1016/j.ctrv.2021.102180 (2021). Bengtsson, A., Andersson, R. & Ansari, D. The actual 5-year survivors of pancreatic ductal adenocarcinoma based on real-world data. Sci Rep 10, 16425, doi: 10.1038/s41598-020-73525-y (2020). Chicklore, S. et al. Quantifying tumour heterogeneity in 18F-FDG PET/CT imaging by texture analysis. Eur J Nucl Med Mol Imaging 40, 133–140, doi: 10.1007/s00259-012-2247-0 (2013). Gillies, R. 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Cancer Epidemiology, Biomarkers & Prevention 16, 546–552, doi: 10.1158/1055-9965.Epi-06-0893 (2007). Additional Declarations No competing interests reported. Supplementary Files Supplementarydata.docx Cite Share Download PDF Status: Published Journal Publication published 22 Nov, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 04 Sep, 2024 Reviews received at journal 29 Aug, 2024 Reviews received at journal 21 Aug, 2024 Reviewers agreed at journal 21 Aug, 2024 Reviewers agreed at journal 19 Aug, 2024 Reviewers invited by journal 18 Aug, 2024 Editor assigned by journal 16 Aug, 2024 Editor invited by journal 08 Aug, 2024 Submission checks completed at journal 07 Aug, 2024 First submitted to journal 30 Jul, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4825555","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":346842410,"identity":"b9e44f08-b60a-4588-ab62-31908f92e139","order_by":0,"name":"Yeon-koo Kang","email":"","orcid":"","institution":"Seoul National University Hospital, Seoul National University College of Medicine","correspondingAuthor":false,"prefix":"","firstName":"Yeon-koo","middleName":"","lastName":"Kang","suffix":""},{"id":346842412,"identity":"ca2b1b24-5411-4365-adb7-f0e8561518ac","order_by":1,"name":"Seunggyun Ha","email":"","orcid":"","institution":"The Catholic University of Korea College of Medicine","correspondingAuthor":false,"prefix":"","firstName":"Seunggyun","middleName":"","lastName":"Ha","suffix":""},{"id":346842413,"identity":"663a3673-59ca-48fb-be7e-1b1ca2fa9d85","order_by":2,"name":"Ji Bong Jeong","email":"","orcid":"","institution":"Seoul Metropolitan Government - Seoul National University Boramae Medical Center, Seoul National University College of Medicine","correspondingAuthor":false,"prefix":"","firstName":"Ji","middleName":"Bong","lastName":"Jeong","suffix":""},{"id":346842414,"identity":"528205cd-fa36-4294-b709-7814c7636c72","order_by":3,"name":"So Won Oh","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAqElEQVRIiWNgGAWjYFCCBCCugHEOEK3lDMlaGNtI0aLbnnz4M++8ujyDA8wPPzCcuUdYi9mZZ2nSvNsOFxscYDOWYLhRTISWGzlmzLnbDiRuOMBgxsDwIYEoLcafc+fUAbWwfyNai4F0bgMzUAsP0JYbxGgB+eXPscOJMw/zFEsknCFGy/Hkwx9n1NQl9h1v3/jhwzEitCAAMwMkWkfBKBgFo2AUUAEAADg8PemxVc+LAAAAAElFTkSuQmCC","orcid":"","institution":"Seoul Metropolitan Government - Seoul National University Boramae Medical Center, Seoul National University College of Medicine","correspondingAuthor":true,"prefix":"","firstName":"So","middleName":"Won","lastName":"Oh","suffix":""}],"badges":[],"createdAt":"2024-07-30 04:24:57","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4825555/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4825555/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-024-77022-4","type":"published","date":"2024-11-22T15:56:59+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":64385581,"identity":"91dd4ec5-dbf6-472d-abc8-b2e11c415242","added_by":"auto","created_at":"2024-09-12 12:27:43","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":607924,"visible":true,"origin":"","legend":"\u003cp\u003eFlowchart depicting patient inclusion\u003c/p\u003e","description":"","filename":"Figure1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4825555/v1/c9f0d0320774c4b9c32740c2.jpg"},{"id":64385579,"identity":"2b4969ed-bfcb-4baa-8f1d-4ba55dde7f83","added_by":"auto","created_at":"2024-09-12 12:27:43","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":283805,"visible":true,"origin":"","legend":"\u003cp\u003eOverall survival of patients with high and low Rad-Score\u003c/p\u003e\n\u003cp\u003eKaplan-Meier survival curves illustrate the overall survival of patients with high (≥2.8) and low (\u0026lt;2.8) Rad-Score determined using the optimal cutoff to produce the highest chi-square value. Patients with higher Rad-score demonstrated significantly worse overall survival.\u003c/p\u003e","description":"","filename":"Figure2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4825555/v1/86a904dc09fb706f672c89db.jpg"},{"id":69834690,"identity":"75c253dd-b958-46d0-8dba-34ae6e8ab6b7","added_by":"auto","created_at":"2024-11-25 16:07:22","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1548809,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4825555/v1/dca0ba40-61c7-4c6f-95bd-de39ad4afaaf.pdf"},{"id":64385580,"identity":"5cd06f63-4e02-43ae-90ce-90ed24e66eff","added_by":"auto","created_at":"2024-09-12 12:27:43","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":41923,"visible":true,"origin":"","legend":"","description":"","filename":"Supplementarydata.docx","url":"https://assets-eu.researchsquare.com/files/rs-4825555/v1/68a2932fffd6fc28abebf4fd.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"The Value of PET/CT Radiomics for Predicting Survival Outcomes in Patients with Pancreatic Ductal Adenocarcinoma","fulltext":[{"header":"Introduction","content":"\u003cp\u003ePancreatic ductal adenocarcinoma (PDAC) is often associated with a poor prognosis due to its aggressive nature and resistance to conventional therapies\u003csup\u003e\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e\u003c/sup\u003e. Nearly half of newly diagnosed patients with PDAC present with distant metastases at the time of primary tumour detection due to early dissemination and high risk of metastasis\u003csup\u003e\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e\u003c/sup\u003e. PDAC tumours often exhibit significant heterogeneity, rendering them refractory to standard chemotherapy and targeted interventions. Despite advancements in surgical techniques, radiation, and chemotherapy, the 5-year survival rate for PDAC remains remarkably low, typically ranging between 10% and 15%\u003csup\u003e3\u003c/sup\u003e. Survival outcomes vary contingent on factors such as the stage of cancer at diagnosis and individual patient characteristics. Consequently, it is important to identify specific tumour characteristics associated with poor prognosis.\u003c/p\u003e \u003cp\u003ePositron Emission Tomography (PET) using F-18 fluorodeoxyglucose (FDG) is a valuable tool in oncology, providing functional imaging data which is critical for assessing tumour characteristics. PET-derived metrics encompassing key parameters such as maximum standardised uptake value, metabolic tumour volume, and total lesion glycolysis, are instrumental in prognosticating therapeutic response. Nonetheless, these parameters may not comprehensively capture the intricacies of spatial tumour complexity which is intrinsically linked with cellular and molecular dynamics, including proliferation and necrosis\u003csup\u003e\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e,\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e\u003c/sup\u003e. Intratumoural heterogeneity (ITH) may have prognostic clinical importance, particularly in appraising resistance to therapeutic interventions. Various image features, such as texture analysis, have been proposed for the evaluation of ITH\u003csup\u003e\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e\u003c/sup\u003e. Therefore, there is a need to quantitatively discern image patterns that have clinical relevance, particularly within the domain of spatial tumoural complexity, in order to discover and develop valuable clinical indicators and models for precise prognostication.\u003c/p\u003e \u003cp\u003eRadiomics analysis has recently emerged as a sophisticated, integrative, data-driven approach for quantifying complex image patterns and constructing clinically valuable models using imaging data. Extending beyond the limits of visual interpretation and basic quantitative assessment\u003csup\u003e\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e\u003c/sup\u003e, this approach employs automated, high-throughput extraction and refinement techniques to analyse a vast spectrum of image patterns from medical images. FDG- PET radiomics represents a noteworthy advancement in the quantitative analysis of imaging data and has attracted attention for its potential in assessing spatial tumoural complexity, including ITH, and predicting therapeutic response in the field of oncology. In this study, we aimed to develop and validate a model based on radiomics analysis of FDG-PET/computed tomography (CT) images of primary tumours to predict the overall survival (OS) of patients newly diagnosed with PDAC.\u003c/p\u003e"},{"header":"Methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003ePatients and Overall Study Design\u003c/h2\u003e \u003cp\u003e This study received institutional review board approval from Seoul Metropolitan Government - Seoul National University, Boramae Medical Center (SMG-SNU BMC; approval number: 30-2022-72), and was performed in accordance with the Declaration of Helsinki. The board granted a waiver of informed consent for this study due to its retrospective design, recognising its minimal risk to participants.\u003c/p\u003e \u003cp\u003eThis retrospective, single-centre, medical record-review study was designed to predict the therapeutic response of patients who underwent FDG-PET/CT for initial staging of PDAC between June 2010 and October 2020. Patients were included if they met the following criteria: (a) the presence of FDG-avid primary tumour; (b) the absence of clinically suspected distant metastases based on CT, MRI, and FDG-PET/CT images; (c) the absence of any other primary malignancy; and (d) no history of receiving any anti-tumour treatment prior to PET/CT imaging, including surgery, chemotherapy, or radiotherapy. Patients were excluded if they had at least one of the following criteria: (a) tumour size too small to evaluate FDG distribution (\u0026lt;\u0026thinsp;64 voxels); (b) PET/CT misregistration due to respiratory motion; and (c) limited delineation of tumour uptake on FDG-PET/CT images due to adjacent activity of the stomach or inflamed pancreas. A flow chart depicting patient selection is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003ePrognostic models were constructed using both clinical parameters and FDG-PET/CT radiomics analysis, which were then validated to predict OS. The commencement of observation was defined as the first date of referral to SNM-SNU BMC for the management of pancreatic cancer. Each patient\u0026rsquo;s survival data was obtained from the national database of the Ministry of the Interior and Safety of South Korea, with December 2020 as the cut-off date. Demographic and clinical characteristic data were extracted from the medical records of each patient from the point of initial PDAC diagnosis.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec4\" class=\"Section2\"\u003e \u003ch2\u003eFDG-PET/CT Imaging\u003c/h2\u003e \u003cp\u003eFasting status was confirmed by blood glucose levels (\u0026lt;\u0026thinsp;140 mg/dL). Subsequently, an intravenous injection of fluorodeoxyglucose (FDG) was administered at a dose of 5.18 MBq/kg. After a 60-minute rest period, a non-contrast CT scan was conducted using parameters of 80 mA and 140 kVp with a slice thickness of 5 mm. This was immediately followed by 1 minute of PET imaging per bed position utilising a dedicated PET/CT scanner (Gemini TF, Philips Healthcare, Cleveland, OH). CT images were reconstructed using a 512 \u0026times; 512 matrix across a 50 cm field-of-view. PET scans covered the area extending from the mid-thigh to the vertex and were reconstructed using a 128 \u0026times; 128 matrix. PET images were processed using the ordered subset expectation maximisation algorithm, with parameters of n subsets and m iterations, enhanced with time-of-flight and point spread function. Additionally, CT-based attenuation correction and application of a Gaussian filter with a full width at half maximum of 8 mm were employed to optimise image clarity and diagnostic accuracy.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec5\" class=\"Section2\"\u003e \u003ch2\u003eFeature Extraction from FDG-PET/CT\u003c/h2\u003e \u003cp\u003eA nuclear medicine physician (Y.K.K) with 10 years of experience performed PET/CT interpretation analysed PET/CT images using free medical image texture analysis software (LIFEx 7.4.0, \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://www.lifexsoft.org\u003c/span\u003e\u003cspan address=\"http://www.lifexsoft.org\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e ) \u003csup\u003e\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e\u003c/sup\u003e. For segmentation of pancreatic tumours, spherical volumes of interest were manually delineated on PET images encompassing the entirety of each tumour, while a contrast-based approach was used to define tumour contours. Normal retroperitoneal fat tissue was excluded from the defined tumour volume via visual assessment of corresponding CT images. Texture parameters were automatically calculated from the final segmented tumour volume derived from PET and CT images. Intensity discretisation was carried out using a fixed bin size method for both PET (bin size, 0.5 standardized uptake value; range, 0\u0026ndash;50) and CT (bin size, 10 Hounsfield unit; range, -150\u0026ndash;150) images. A total of 222 texture parameters were used as input factors of the radiomics model. The extracted features were grouped under the following heading: including morphology, intensity, local intensity, intensity histogram, grey level co-occurrence, grey level run length, grey level size zone, and neighbourhood grey level difference features \u003cb\u003e(Table \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e).\u003c/b\u003e\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec6\" class=\"Section2\"\u003e \u003ch2\u003eDevelopment of a Radiomics Model\u003c/h2\u003e \u003cp\u003eA radiomics model was developed to predict OS. To identify parameters with significant prognostic influence among the extracted texture features, we employed the least absolute shrinkage and selection operator (LASSO) method for variable selection and shrinkage within the Cox proportional hazards regression analysis. We conducted 10-fold cross-validation on the entire patient cohort to minimize overfitting and optimise model hyperparameters. The model was optimised to maximise the Harrell\u0026rsquo;s concordance index (C-index). A radiomics score (Rad-score) was then produced for each patient by summing the selected prognostic variables weighted by their coefficients obtained from the LASSO Cox regression analysis. The Rad-score was used for the subsequent survival analysis.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec7\" class=\"Section2\"\u003e \u003ch2\u003eStatistical Analysis\u003c/h2\u003e \u003cp\u003eClinical characteristics were compared between patients who survived and those who did not using Student\u0026rsquo;s t-test for continuous variables and chi-square test for categorical variables. The prognostic significance of clinical variables and the Rad-score for OS were assessed by univariate and multivariate Cox proportional hazards regression analyses, respectively. Harrell's C-index was used to represent the prognostic values of Cox models based on clinical variables (clinical model), Rad-score (Rad-score model), and a combination of both factors (clinical\u0026thinsp;+\u0026thinsp;Rad-score model). The difference in Harrell's C-index between the clinical model and the clinical\u0026thinsp;+\u0026thinsp;Rad-score model was assessed using bootstrapping with 1,000 resamples to determine the additive value of the Rad-score. Radiomics model development and statistical analyses were performed using R software 4.3.3 (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://www.R-project.org\u003c/span\u003e\u003cspan address=\"http://www.R-project.org\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e). LASSO Cox regression analyses were performed using the \"glmnet\" package embedded in R software (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://cran.r-project.org/web/packages/glmnet\u003c/span\u003e\u003cspan address=\"https://cran.r-project.org/web/packages/glmnet\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e). A two-sided P-value below 0.05 was considered statistically significant. In the multivariate Cox analyses, variables were included in the model if their p-value was less than 0.05 and were removed if their p-value exceeded 0.1.\u003c/p\u003e \u003c/div\u003e"},{"header":"Results","content":"\u003cdiv id=\"Sec9\" class=\"Section2\"\u003e \u003ch2\u003ePatient Demographics and Clinical Characteristics\u003c/h2\u003e \u003cp\u003eThe study included 84 male and 56 female patients diagnosed with PDAC, with an average age of 69.0\u0026thinsp;\u0026plusmn;\u0026thinsp;10.6 years. The clinical staging distribution was as follows: T1 stage, 10.7% (n\u0026thinsp;=\u0026thinsp;15); T2, 27.9% (n\u0026thinsp;=\u0026thinsp;39); T3, 25.0% (n\u0026thinsp;=\u0026thinsp;35); and T4, 36.5% (n\u0026thinsp;=\u0026thinsp;51). A considerable majority (75.0%) of patients had no suspected regional lymph node metastases. Most patients (78.6%; n\u0026thinsp;=\u0026thinsp;110) received initial treatment at SNM-SNU BMC, including upfront surgery (36.36%; n\u0026thinsp;=\u0026thinsp;40), systemic chemotherapy (30.91%; n\u0026thinsp;=\u0026thinsp;34), and best supportive care alone (32.73%; n\u0026thinsp;=\u0026thinsp;36). The Ministry of the Interior and Safety of South Korea\u0026rsquo;s national statistical database indicated that, among the included patients, 102 patients (72.8%) were deceased at the cut-off timepoint, with an average follow-up duration of 19.5\u0026thinsp;\u0026plusmn;\u0026thinsp;19.2 months for the entire cohort, and 34.4\u0026thinsp;\u0026plusmn;\u0026thinsp;28.8 months for survivors. No significant differences in demographic or clinical characteristics, including age, sex, smoking history, and clinical staging, were noted between survivors and non-survivors \u003cb\u003e(\u003c/b\u003eTable\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e\u003cb\u003e).\u003c/b\u003e\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eClinical characteristics of patients.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003eFactor\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eSurvived (N\u0026thinsp;=\u0026thinsp;38)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eDeceased (N\u0026thinsp;=\u0026thinsp;102)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eTotal (N\u0026thinsp;=\u0026thinsp;140)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003e\u003cem\u003eP\u003c/em\u003e*\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eAge (year)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e67.3\u0026thinsp;\u0026plusmn;\u0026thinsp;9.4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e69.7\u0026thinsp;\u0026plusmn;\u0026thinsp;11.0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e69.0\u0026thinsp;\u0026plusmn;\u0026thinsp;10.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.241\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eSex (Male: Female)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e22:16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e62:40\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e84:56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.847\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFollow-up periods (months)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e34.4\u0026thinsp;\u0026plusmn;\u0026thinsp;28.8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e14.0\u0026thinsp;\u0026plusmn;\u0026thinsp;9.4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e19.5\u0026thinsp;\u0026plusmn;\u0026thinsp;19.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003e\u003cb\u003eSmoking history\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eSmoker or ex-smoker\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e5 (13.2%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e25 (24.5%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e30\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003e0.147\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eNever-smoker\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e33 (86.8%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e77 (75.5%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e110\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\" morerows=\"3\" rowspan=\"4\"\u003e \u003cp\u003e\u003cb\u003eClinical T stage\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eT1\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e4 (10.5%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e11 (10.8%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e15 (10.7%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\" morerows=\"3\" rowspan=\"4\"\u003e \u003cp\u003e0.133\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eT2\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e16 (42.1%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e23 (22.5%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e39 (27.9%)\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eT3\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e8 (21.1%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e27 (26.5%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e35 (25.0%)\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eT4\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e10 (26.3%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e41 (40.2%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e51 (36.5%)\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\" morerows=\"2\" rowspan=\"3\"\u003e \u003cp\u003e\u003cb\u003eClinical N stage\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eN0\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e26 (68.4%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e79 (77.5%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e105 (75.0%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\" morerows=\"2\" rowspan=\"3\"\u003e \u003cp\u003e0.158\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eN1\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e11 (28.9%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e16 (15.7%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e27 (19.3%)\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eN2\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e1 (2.6%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e7 (6.9%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8 (5.7%)\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eHaemoglobin (g/dL)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e13.1\u0026thinsp;\u0026plusmn;\u0026thinsp;1.8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e12.5\u0026thinsp;\u0026plusmn;\u0026thinsp;2.0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e12.7\u0026thinsp;\u0026plusmn;\u0026thinsp;1.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.096\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eWBC (10\u003c/b\u003e\u003csup\u003e\u003cb\u003e9\u003c/b\u003e\u003c/sup\u003e\u003cb\u003e/L)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e7.1\u0026thinsp;\u0026plusmn;\u0026thinsp;3.0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e7.2\u0026thinsp;\u0026plusmn;\u0026thinsp;2.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.2\u0026thinsp;\u0026plusmn;\u0026thinsp;2.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.786\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003ePlatelet (10\u003c/b\u003e\u003csup\u003e\u003cb\u003e3\u003c/b\u003e\u003c/sup\u003e\u003cb\u003e/\u0026micro;L)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e243.2\u0026thinsp;\u0026plusmn;\u0026thinsp;62.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e260.0\u0026thinsp;\u0026plusmn;\u0026thinsp;85.0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e255.2\u0026thinsp;\u0026plusmn;\u0026thinsp;79.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.277\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eAST (IU/L)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e148.5\u0026thinsp;\u0026plusmn;\u0026thinsp;224.8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e122.8\u0026thinsp;\u0026plusmn;\u0026thinsp;149.3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e129.7\u0026thinsp;\u0026plusmn;\u0026thinsp;172.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.435\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eALT (IU/L)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e166.2\u0026thinsp;\u0026plusmn;\u0026thinsp;202.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e134.4\u0026thinsp;\u0026plusmn;\u0026thinsp;171.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e143.1\u0026thinsp;\u0026plusmn;\u0026thinsp;180.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.357\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eALP (IU/L)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e303.9\u0026thinsp;\u0026plusmn;\u0026thinsp;224.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e388.9\u0026thinsp;\u0026plusmn;\u0026thinsp;387.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e365.9\u0026thinsp;\u0026plusmn;\u0026thinsp;352.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.111\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eTotal bilirubin (mg/dL)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e8.7\u0026thinsp;\u0026plusmn;\u0026thinsp;9.4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e7.5\u0026thinsp;\u0026plusmn;\u0026thinsp;9.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.8\u0026thinsp;\u0026plusmn;\u0026thinsp;9.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.491\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eCEA (ng/mL)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e3.6\u0026thinsp;\u0026plusmn;\u0026thinsp;4.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e8.5\u0026thinsp;\u0026plusmn;\u0026thinsp;23.6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.1\u0026thinsp;\u0026plusmn;\u0026thinsp;20.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.052\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e \u003cp\u003e\u003cb\u003eCA19-9 (U/mL)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e2,085.8\u0026thinsp;\u0026plusmn;\u0026thinsp;7,151.4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2,427.6\u0026thinsp;\u0026plusmn;\u0026thinsp;12,330.4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2,333.5\u0026thinsp;\u0026plusmn;\u0026thinsp;11,122.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.873\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003ctfoot\u003e \u003ctr\u003e\u003ctd colspan=\"6\"\u003e*Student\u0026rsquo;s t tests for continuous parameters and chi square tests for categorical parameters were performed.\u003c/td\u003e\u003c/tr\u003e \u003ctr\u003e\u003ctd colspan=\"6\"\u003eWBC, white blood cell; AST, aspartate transaminase; ALT, alanine transferase; ALP, alkaline phosphatase; CEA, carcinoembryonic antigen; CA19-9, carbohydrate antigen 19\u0026thinsp;\u0026minus;\u0026thinsp;9\u003c/td\u003e\u003c/tr\u003e \u003c/tfoot\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec10\" class=\"Section2\"\u003e \u003ch2\u003e\u003cem\u003eRadiomics Model Development\u003c/em\u003e\u003c/h2\u003e \u003cp\u003eLASSO Cox proportional hazards regression model assisted with optimal hyperparameters selected seven features of PET images and four features of CT images from among the 222 texture features extracted from PET/CT images (Figure \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e in Supplementary data) to construct the radiomics model. Details of the selected features and Rad-score calculation are as follows:\u003c/p\u003e\u003cp\u003e\u003cimg 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lKEfu+TBbG3Df2pHYyuCjDjW1P+lsl3QTtd6gJ614+lax0HhAoXJwUAegiYUSZTy4NChQymPCVlCE16LgWRQD7hXO3EpS93SpNW9KCvqzHkfLHoq30XuC2xjwWITKLXDfZVn0WkjX/S00KmuZGuD6fIbZaibL+Rck9+lh9rtKjMp9FNbIIA9+CAn2l6C8UCZNmbjC2QzVtXnJej3OCaRR9m8vS6oLz1K4xvwTe1cAXTG30OQndHWUh5cybmf143oXhwjyFV+LaoTfVbK45y+0riL94QCzLweeW39CwQ19LN8BpyTp4CnDXStCbKRhx61vlf/l+YJOsnW6P8Sk9pGsIhdlMWHJb2Za+gQZVOHuVmL+hSQU2qH8cd4j+1cr9TPDDOvYKKWFq0uSoud8vJJpnwtRiwQbQtT3FwmmVSq27UxxIVLCzqpBuqqvBahEpJLGWzCJyxqbeXzzZN2ShupNg0tRhyRLZ206FKuZrFTOwpOaoIgoNyQxbQG2sSeLr9qfMRP4tRh0+hCm00Xk/pCSLccNizy8zFJnvqxD3ShfEwl8MuQfkHn+TD3u+rqXj6fNJeG2M8Yok7sN+WVghbKtbWBH7iHj0RX+UgMnkicdwVNgv7R+O6ibczm0DZlsKUklzz1N/Loiz4mtU3gu9K8ktyI/F0L/qO8UqkdiOvI9YyDwgXKJBsDkytOCiYAE4G8fCFR2Zjiph7RJM0nbw01G0NbGmI/i4vqtQUNyIvySW02gzbPmEqbp+TiJxZuLT6qg52ylWMNCoawq82eHMoOHTNdyC7ax7a8/yLYRTnZzFjpKo89lKuxaxJfCPk9p60/yJumD0FtdfkjQvmhOlyLc7+rru61JdnRBz5VHfzGtWRjf+nVpu6XfKwxH9tX+RofKMghcd6HAr18HJZg7SmtPxGNNdmXy6U9dNNYrG0bhtoWQZdSn5Kf94P8XatXLbQTg/3rFQeFCwRNhL7URlwclVgUWUTyyZUvpMBEb9tsmUyUnWRCdS2oupcvCtrMSotIF2x+qssxh3wSNsumric46Bz1wP7S5ilZlGOxlD2xPdoZupDKP7V+oN1SWdqXLl0pHydCfs37STCesE++4RpbqaPNJ0Ie94Z8ah/qCyHbc5Sf20xe38Y7lLa2hGzrS23E+ayEf6/23O+qq3uxP9Eh160PxqbqKGmulQJCkL9L47mkV1f5HI3ttrGfo7HRBzrQTzUyAdvxAbKjL/O+1n3k9/l8qG0RfBd9KsjP/Sp/D/nwV0OpresRB4ULFCbokAEcF8e+CauFiFSDyuZPHGrABurO5cYQyZ/QidzmtnKRvvtCduSLsOpj39CFVH5QcFWzQKJHaeGdBvQf+pSgzXysYivl8yCafMr2+TQyiS+E+j1HMvMxTd60fSgdam2mXO7PLq7Vud9VV/eiTxgryq9F47L0obMN+pc6JR+Txz3Gmugqn0Mgji6kKKMN9UcXrFXIGrJ+gOagxnP0byn1jc+htkXwXWleSWZE/p426FDTh9c6/kkaU8WLL76YjqMJln6vqYuTJ0+m42iCpCPwcwZLCj9PEH9WZPXq1eOzeo4ePZqOa9asSceI7ul3peD1118fn330O3B98FMG8WcaPvGJT4zPmuamm24an11uMz/7MAow0vmvf/3rdIxEP0Q98vYi/BzNaLG8TO+f/exnzU9/+tPefhH8tMK6deua0WaQfuaExDX5ffzlL38Zn02XG264YXxWpu0nX6I+lMF/3//+9y/6SD+/0cZsfNEFv2tG/7/22mvjnI9+igRuv/32dLxeuBbnfpSV122bW6+88ko6Rt36ePbZZ9ORn3OZBuvXr0/H+FM4/EA5fOlLX0rHNvjZkzNnzqSfgiK99dZb1T+F0jZ/WFOefvrpNO7pW8Z918/SRPKxwM/djGKKSxKMgsF03rXezsa2LpjLb7/99iX2v//++2ksmxZGnWUWIHxqq/1UwydCnqAwXGpe8/DJjLLxkxuf/ErDjU9y5JOGflKNTzDyVzltTzekR63t2I09yCCpfu4H2Rzz9ZQh1wF0L+qhp4v5J2rKkI8uUY5k19oCPMFCDm1FuCa/6ykZtg9pqw3GBa/g1Gcc89ff0S497Si9Po79zliKdlGOMm3MxhdC4ywff0AfS450ZpxMG/knH2NtzIe5r/nDuMnRvdzXlCW/9ESphPxKqn2SiR1xvubjgmv5k3GhMUjq8oHGTizDOXlx3uTQHm3lawrQdi4T38g/HGNdzqPvdL809gG53M/nV86ktkXaysrfuoctXNf25xDo95o5cq3joHCBMmRj0CKnVFpgRNcrhLw9LSpKtQsAMNm1yJPiYtV2j8VJeSwMbYtZhI0ryuI8tgXRDu5Lbtz0WLSEFibdy1OO/J+3q/I1wYtg0WpbpMnvWtS4j32zhQWfduRXfMF13BTIj+OFRRwfymZ8G+2OHwLy1MZsfRHnBTZwHXXCHsa0yqBzzZgbCuNiSL9c73Ofvo7zJ+rUdi/2Q5yLXai8Uh+5r2KKKBjTvXws5zBmkB3nhyCPe13jirFRGudRh5i0zqiP5EN8m69pXeMhzu82+2ZrG/fVjnTK5y16qwxHfbicNshvW0+uJxbxz8gYs8DgFQv/p+Ns/lcKs7DgFcwnP/nJ5re//W3nqyBzZeF/xPjKV76S/peTGjz3Fxa8Dn755Zfd33MIX4X46le/2rz77rvVX+O5VvF3ChcobOrXwyKh/54oT/n3k2YDskpt0Lb5GBa7733veymgMNcGbEYXLlxoHnjggXFOP577H7MQ5j5j480335yq38yl/PCHPxz0ve5rGT8pNMYMQl8A58vp5urBHwXcd999zQsvvJD+sMWYNjxW5o75th76SaExZhAsfvfcc4+fpF5F9u/fn9KxY8e8yZteGCOMFcYMf3FsZo9+7YC1cD59QPaTQmOMMcYY4yeFxhhjjDHGQaExxhhjjBnhoNAYY4wxxjgoNMYYY4wxDgqNMcYYY8wIB4XGGGOMMcZBoTHGGGOMcVBojDHGGGNGOCg0xhhjjDEOCo0xxhhjjINCY4wxxhgzwkGhMcYYY4xxUGiMMcYYYxwUGmOMMcaYEQ4KjTHGGGOMg0JjjDHGGOOg0BhjjDHGjHBQaIwxxhhjHBQaY4wxxhgHhcYYc81x/vz5Zvv27SmdPXt2nGuMMXPLopkR43NjjDFXGQLC1atXNy+88EKzYsWKca4xxsw9flJojJkTTpw40WzZsqU5ffr0OMfU8IMf/KB59NFHm//7v/+7xHeHDx9O/sSvxhgzJ/Ck0Cw8jh8/PrNx48bxVR2UZ8jkafPmzTOnTp0al/qI3bt3F8uSVq5cmcq0yVNatmxZKtcFdsQ60aa2e3n+gQMHUr6ZHviUfj537tw4J72RKKZt27aNS1zOoUOH0jigHMe8r86cOZPqL168+GIZ6sw1jO+dO3eOry6lT2fZnSeBLdRjvHIe28Fe/DrbMYv+pCGUdEY//B/7Gbrmtuwp3Yupa1zkaLzF+nEtgFwn1oES2IJv1IckZMdxlcuKUF/jkYScPuKaRN3SvCE/onylSMn/2IBdeV9BLJf7LV8vh/RLLbTBPhJ1yPeUCPdieXycj+dcJvZ3yTQf4aBwgcKEySd/H0woTTDqgyZdvvCxeeVlQQsTaEMBLWLaMCiH7BqQIR3yBa/tnnSrbWMhQd/RH6XNgzzuUaYN+jvf2IA+jXmUow/aZOm+AiDGDddxPHG9b9++dI5s2ii1LWZrG3OANmg334SgRuc+KC/90IXrCPewcTYbHHqV9O8CP6OL9EEP5hXXeaCA/bEsUJ5rfKG1RH5SWQIvlVO/dkFZ9QdzWX2HPuRH5Msu2eiFbymDf5Cf9ykoTymOGa05SrX9pDWQFNvSGMrlRB933QP0k/zcL6A2SPlarj7WPXxSgrU76h0hv+1DFL7E55oj6gP0LUH73EeedJG9aj+Xif3onttmLsdB4QKFydI26dqgjhYHUcoDFnfymJglmMy0r0ktGfo0zr3aTUuLXWnR0b0oK+qsRcNcijbVuAFwTl4eAOTg85oNnU28KygvbeyMp672Ne66+nVS29hY0JeybeNzEp1zqC//YU9pI2Oso8OkoHvt/BKUx7ex3VIeoB/5bf1L/8gncT6qTzivmZvIp2zu8xKldiLkKQDK1xLyYuClflF56ao28AfHrvGdgw3UUX2Bj0t9Ld+XbJf/Y70YFGudFRpPui9oI9rZNa8VzOWBIdfk54FrF9K/hHyc9yH6o28b3B/SHwsVB4ULFCZWaaHporTQKI9FI6J8TUIWk64NgrKliV6D6uYLHeheXJC0mJJqoT76s7hRD3u1+GmDIB8746dy8rmOTCor7y98pUVbcqiDXPlRCzL3tWBGf3PeRgyeSJz3BTfaeHKbc1Suq33sYRxF8EHuhwj2IrdvHE1iWwQdShvQJDrnMD5in5Y2U/Jq/NwGupf07wIf0Wasp7zcd8pXEMF413kO8ihLnSHUjmPR147ux/nTBn2MTfRrbJ/+Qo7mXJvNObRHefzIkaS+bRtrajsfb1DqK5DsPJ/y6Kr7oDka80tjMcJ9bNd6pvWnr16O5mcJfIVMyqif8D95pflA29hLf/X1q3FQuGBhEg3ZqCBfaGLAkQdkKhvTtDcFiBtDPuHjvVKqtR/bKI9+LDpaLKnPOYuT2qIM9qBL7i+YVFYuBySfOqBAnHyQPAVKJEF7bGB9UB95JLXThdrqA1l9/kdObjN12uTjJ2yq3YiH2hZBj1w3GKrzbEBmPu9qQceS/m1oDJIYn1xTn+t8M45lY2oLDOSfUnDThcZ7zTiGvnYYB9yvGQuUZZxJpvypIJ48Um0wpHUh+g75usbnOSqXj4G8ryLKj3NE5aPe1MM2fEFZ8ujnGpCjoI1jrQ+E9Oiqh874Wvq2taP+4T79Qz3TjYPCBYIW8L7URlxolJhofPrKF57SosQi2jbJJ90UQHYhP0f3kB9Bb/K5X4PKoz8Jm3N9FQzFDUqLVly0J5WVo4U6lpG9uR9L/UE92umDuuhMqllQ0SH3d44C4b6AhjJ5HyEbXUpwb0hwN9S2CG2Vxs9QnWdDqa020IHyXYkybShoiYkxhL9jQAixLMjPbahs33jIyYM42pEsUm6P8tva0f2+eaF2mL/4n3MFQMyv0tzsgzkrfZFFfeyTL/PxSTvkl+7l/hexjtYBoLz6R+MEfTQvtEYNmVvyS+34FLRHu319gE7oI9vl82hXRDZ2jXHzEQ4KFyhMniETJC40+SKUExefGlSWNoYSF7Ec3YsLEwu52mtbQCLRFhILPQtSvmhpIZceCnxI8tdQWV0LqmyLn/i1SZb8qHu0RZ3avkc/6pJqNgV07pONnJoNk0U871dkl+QjM/qihqG2RdCh1D9DdJ4tfWOkC+oNqYtNtIev+kAuZWtsjnOib13JUb1oh/RUQCP62on3Oe+COYR8kK0kxhNw5HrImGI+aPxGXZBR8rna7bqX+x9Z5Odzj3zpTh21LX2wleu+QE1ojUUudbmugX6h/T7/yz85+EJ2lNDYMN34dwpNFS+++GI6jiZes3Tp0nTexsmTJ9NxNMHTEfhB3iVLllz2G2v89prgB3uHcvTo0XRcs2ZNOkZ07+67705HeP3118dnTbN27drxWTvRltF8ad57773m4MGD6ffiIseOHUtH6fHSSy+lI/Xkr1pZ5EHUO0e2ffrTn05Hfs/u7bffTuef+tSn0jGyYcOGdPzNb37TPPzww81o40jXXfC/aYyC2+bIkSMpvfXWWymvD/q6De5hH7/D1wc6y68Cn91xxx3jq49Ap1tuuaXZunVrut6/f3/vb/lNalsftTpfbzz77LPpqHHUxcsvv5yO69evT0egPxYtWjS++pjf/e536VizruSMgpvx2cfQp4CeUZ7mXmyH/ylGOt10003pmMNY3bRp0/jqI1599dWLc/b2229Px1Hw0zzxxBPpXP1/zz33pGMf6IHed911V7pmXZJtTz75ZNHn8nHXvTjmWB+QBb/4xS/SUaDvF7/4xXSuOqxRzCfqXbhwIeXdeeed6dgF5detW9eMAshm7969KXEdf2+zBOsCfv7+979/cV3O18Wc0jrz5z//eXx2OTfccMP4zHSSQkOz4ODT1mjij6+64ROcPi3mT0FK8ImNsvETvD6l5uhTNWnok4L49JInc5G2J5vSo9Z2fSrl0zVySHxijp9I49NHtaVP6/iL8qRJZZVABmWwkzryI/kloj9qnqjhJ/ox6sA5edxrA3toow38oqcsOfRJrCudkQnoTd3Y1+Tl+iCn62nDpLZF2srW6DwtaIf2JoF+iPOzC43bmvbwo8qqD+TbfM6Rr3HM/aFojmn+RD3lf4F88rV+lXTS+NP8QB59F9c86sU8tak6HKVDbd/IDmSLLjmU073czniPelxTBp3Jy/tc+qsN6aKne0PWZ+rQTq6T2pfMErQT69EWdURcG3SP+Sed5K9oB2NL844j17XzeyHjoHCBwmKQL9JtaEIqUbeNuJjlKW9PC5DSkAmrSa66cbFru8eiozwWldqNmvqUVz30jHVlc9zYWJwoS4qbyiSySmihlXzVa/MhbXTdj1CWviptAuRxr813aqftPvrGvoponEWiv/BJHHu0oXt5ahujs7ENuB/HFjrF/oUunacFMpHfpWsX6NjWDzmyVamLGETkKW9P/a2UBxM14Hv5mn7hmmP0S9eaFHWi/3P90TH2X9Q5H0Nc53X7UD+S4tyMsvJ2og55GyX/4w/yS+NQZUrrDWtYlNM3XvB9Wx+Sn88TkbcTk5DNIn4QJqG/AkLAZxoL3GeMcJ370lyO/+/jBQqvc3iFx6szc/2g1yw/+tGPms997nPj3KZZvnx5egU1WnyLr1327NnTPPfcc6m/h76mGwr68epwx44d4xwzbXbt2tX8/ve/n3j+Mh7AfWSMuYQUGhpzjRI/Fcc0zacv8dN6TDWf9K80fFpHN55+AJ98ecJAXv5pn0/SfHrmUzr3rtSnZPzJJ3N/Kp8beAqGf7tex80X4nyM6XpD8zZPfU/fjLnS+EmhMdcRPCn81re+lb4cri+AL1u2rPnGN77RPPDAA5c8BVy1alX645NR0Ng88sgjc/6EMMIX9B977LEr8mRyIaEnxd/5znd6v4hvjDFDcVBojJkT+IrCL3/5y/TXzitWrBjnmkmRPx988MGqv5w3xpihOCg0xhhjjDGNf6fQGGOMMcY4KDTGGGOMMQ4KjTHGGGPMCAeFxhhjjDHGQaExxhhjjHFQaIwxxhhjRjgoNMYYY4wxDgqNMcYYY4yDQmOMMcYYM8JBoTHGGGOMcVBojDHGGGMcFBpjjDHGmBEOCo0xxhhjjINCY4wxxhjjoNAYY4wxxoxwUGiMMcYYYxwUGmOMMcYYB4XGGGOMMWaEg0JjjDHGGOOg0BhjjDHGOCg0xpgFyYkTJ5pNmzalozHGgINCY4xZYBw8eLD505/+1Lz33nvjHGOMcVBojLmGOHv2bLN9+/YUtJi5Y8uWLSktX758nPMR+J+nh/a/MQsTB4ULFL06GgLlFy1adFliczl9+vS41Efs2bOnWJa0atWqVKZNnlK+YZXADpVfsmRJc/78+fGd5pL8HOqht8qQ0Et2xPxSInARUQcl2kR+6dVc7pu8TO4XNuo+SvbgP+wp6ZenGAQM6edpguwNGzY0W7duTW3NBsYBMg4fPjzO6Yf26be8P5BFn+FP/ECZXbt2je/OHbTZ1g66qp9K+sR+i6mPW2+9tXnmmWeaV1999ZIxboxZIMyYBcnx48dnNm7cOL6q49SpUzMMGRL1YfPmzel62bJl6VqcOXPmsrKwcuXKmd27d6fzxYsXz2zbti2dowtld+7cma4ph+waVJd04MCBce5Maoc89I7QJvm0Id2oR965c+cu2ilZnJMOHTqU7nO+b9++dE9gP/myTfJUL0f3SFHnWI+Ut1MCGyiLDvgdJAeQga+xS2VJ2IJunEcflfqZfuE672dBu/QDMnPI4550K0EZdIxjZVKwCVlR/z5on/FQqoPujBnZpnEV+y0yW1/gf+mi8RSRrzR31Kelsn2gS5uPuFcz/owx8wcHhQsUNgIW/SFo8yGJUh4o2GDzKqHNURunZCiA4l7tJqcNlBRton5uI5uc9GrbmLEp33BJUVfyBfkqE/Nj8BlReXTjKDvJRy/lt/kuR+3ktgps1uZOW1En2ReJNotSXg56IFd+As7Jkz/bQK/aDwFdEKjJn+ga+6ML9NPY6KsjX8RAOmdSXzAm8QNl6U/ZElHAH2VTpy1gb4P61Cl9aAEF17EdY8z8pn2FN/MaNra2IKINPS2K9dqeIClfGz0bbtumHwOOoRsQ5anHRisZCvbyTZWybHKUKW22JShHeQVRJRQAkyKqm+drs9V96YIN6IyfyG/zV46CmSirDeRTTk9kS6jvos1qoy/wiMEQqS8IEshte/I2KehbExTSrnzdVQd76DvK1ug6qS9EPn6F5EY0lmpReaU4pyOM02n3izHm2sXfKTTVHDt2LB3Xr1+fjnwP7cknn0znv/jFL9JRqOyzzz6bvsv0zW9+s1m3bl3Kyzl58mQ6jja6ZunSpem8ljfeeCMdH3nkkXSEl156KX0P7OjRo83dd989zv2o7IULF9L5l7/85XTs4+WXX05HvuvWxmuvvZaOo401HXNGG+v47CMoH+XRBt9jw5d79+696Ls2f+VgC76Dhx56KH03Mn63MoJPYM2aNelYQu1LR76X9/DDDyc7XnjhhZTXBvrTPt93I3FOXhd8Z3IUyDef+MQnxjlXDtp+7LHHmieeeGKcU4b+ufHGG5t/+Id/aP785z9X6TqJL2pA57Z5kn8fso0dO3YQQV5MR44cGd+5lDvvvLP5z//8z/GVMWbeQ2Ro5j/5k4G21AZPOvKyPEXgqUn+ZCWW1T2ebLS9bqt5etUGdfSUgycoyKEtPb1DF6EnYPFpF2XJU8pRPvLakIz8qY7syp/CUJ4nb3pCyn3yqB+/z9f1vbMSsY9LTxnVHin6JVLqZxK+bauTQznGBqmmjvQqIR92pdy/gnv52MzB73Fcqg6+LNWlTzTO+mTDUF9EsCsfU0B+brP6vuuV9iSU2jLGzF8cFC5Q2NCGLPbxFWnf5oZsla1BZbsCrzYI8AiwILZbesWGvdyLdke78k0+yuuyuVSf4EH58fWb8tm8o3zsoI3a17RtKGAh5ShwyP0Sif5AV+lDUFMbpBKQ0gYJffqQH6YNMvM+jci2ttRWl37ifilgyxnqiwjjtNSGZEbUt9MGHYasE8aY6xu/PjZVvPjii+k42ox6X/HqdfBoM0lH4HXmksLPfcSfDFm9evX4rA69drzrrrvS9dq1a5tRMJXOeRWbv/Ll5zZyPvjgg3QcBT2pfuR3v/tdOnbZHO257bbbxmdNs3PnznSkbvx5lTfffDPpuGLFikvKP/3006mNV155JV13va6O8Go+6nDLLbeko/wQee6559KxS3bsZ/zFz8MAr915Ld8HP2NCn/A6kvTWW29V/7RJ2yvvuQLbRmvgJQlGwWA6z8eDqP2Kw2x80QVfK3j77bcv8df7779/8SsExhgzKQ4KTS9sPvodu5pgRcGHvnsIP/jBD1JgkW+0v/rVr8Znw3n++efTMX6/61/+5V/GZ5d/b+7+++9PR/4XB2wiqPz5z3+e8vLfxeM+34fsIw8c+f04ZFGXvKeeeirdFwR/BKCg4GLz5s3JL9RVmyrTBeXh17/+dTpij3yff8eTsgQSXbT1s4L7//iP/7gkEMkh4CHwIQDCNlJNMHTTTTel4x/+8Id0nAbSk/+1I6LfiCx9966tDuWpJ3Te9b3USX0RQR+CvZx77703jQ/mFGAL/fajH/0oXU+bO+64Y3xmjJn3jD4RmwUIr8ZqXwtRjqGiNOkrubw9vfJSGvJ6DR1K9fRqj1R65curXF7Ncp9XonzPkOv81XVuc+kvMNEBGbEcibrYlrcf7S29Kh8FkZfI6YPXubQVdUBGSXaUSyq9Cm7r59inpdeZIF1KPiePe6U2BX0wjb9y5bV87hOuJVt9UBrDcVzE7+bhT17ZSh4+7poDs/UF96WL2su/b4tOKsORPpoLkD+NfjHGXB8s4p/RxDcLDJ4uPProo+nphTFXG56+8VfYHo/XDny146tf/Wrz7rvvXnyqbYyZ3/j18QKF15XXwwas/8orT6XXf/MVbC35AN/MFx544IH0fcuF1K/XOj/84Q+bn/70pw4IjVlA+EmhMeaagO893nfffem3EPlDHHP10Pcep/G7isaY6wc/KTTGXBMQCPLD2fv377/4By/mysIft/AE+p577nFAaMwCxE8KjTHGGGOMnxQaY4wxxhgHhcYYY4wxZoSDQmOMMcYY46DQGGOMMcY4KDTGGGOMMSMcFBpjjDHGGAeFxhhjjDHGQaExxhhjjBnhoNAYY4wxxjgoNMYYY4wxDgqNMcYYY8wIB4XGGGOMMcZBoTHGGGOMcVBojDHGGGNGOCg0xhhjjDEOCo0xxhhjjINCY4wxxhgzwkGhMcYYY4xxUGiMMcYYYxwUGmOMMcaYEQ4KjTHGVHP+/PlmyZIlzYkTJ8Y5xphpcfbs2Wb79u3NokWL0jw7fPjw+M6VwUGhMcaYar71rW81y5YtG18ZY6bJ888/36xYsaKZmZlptm3b1vziF78Y37kyOCg0xlz3HDx4sNm0adP46urAp/v5zq5du5oHH3ywWbp06TjnI/bs2ZPs5ymHmb9cC/NsvrNjx45m69at6fzv/u7vmvXr16dz2LJlS5prPK2fKxwULlB49TN0clOeR9p5YqCePn16XOojGLilsqRVq1alMm3ylJYvX57K1YA96BHr007UK9eJ6xwmG/m0HcuyGQo92i8ldBDoFO/l7eX6sOD2kfus9Aov1z2m/fv3j0vVyRLohj9j+Xz85PbEpD6fC+iPV199tTly5Mg4Z3KwIfZ1G6Wx2xYQMaawP+//uYDxTlulTYO8OHYZq7FcW/9Jb15j3XDDDc3atWvT9V//+teL44mN7P777282bNhw2VrQB/In8Y1es/GKTbrSL+S32RJTTQBbWleYX9HGeI8EyI7zMI6pfF3IE32Sz2HpylF5IsrL5+Q0mcY8y9cRZJbGKnT5qbRW0uc1c3cazGaeldYOUr7+Uuc3v/lNmlsCu5mDyGjz26yZMQuS48ePz2zcuHF8VcepU6dmGDIk6sPmzZvT9bJly9K1OHPmzGVlYeXKlTO7d+9O54sXL57Ztm1bOkcXyu7cuTNdUw7ZNSCDutRRWwcOHEh5586dS9cCPclvky09pCP1o87ANeUkW+1jT96e5JFkK0T/kGptjfX27ds3zv0Y2uee/Cj9ycv7u08WxProSB3AFvIjNX2eg570VQnyZUcbyM3tmgTGtuxs0zVCmzXlQOOjr/w0fCH/l0BnbKRP6SvmwhDfUVbylXKb6Hfk5vOgC2TU+lJofjPn5DPZj22MVdmqfI1X/Ei9PrCFetijca92I8ojMY6AdmmDVJpbWoei3eil/jh06NBFmZwLjSVShGvsk5450xhbQ8ZKCWREHeW36IMI/o9rJlAW30WGzt1p+KLUB2K28wyoi+1t8wgdaveMoTgoXKAw4YYOVC2ScTKU8kCLWtviy2CnfQ16ydACyL2aCc6Cq3baFsRI3k6E+rqPXW1wP1/YSNoQIiwO2ML96G/Old9Wt0T0d2nB4H5cNLWJkJeX75MFLDzcx44++vq8BHZTPl+kuSa/yy/o3FemBvoSO5FXO+5qy2EHsmvKz8YXbGT4X32ao7Edx73mTs28ycGetjnCWKnxjaDskPL4Ab1ze9AHPwHjXvppnmmzpy3y+tDc6SuLvFhO/ch1ybeMM+nf1qexjOxQ3yoJ9WNbf8Bsxta05lkJrY+1oEcMsieZu7PxxZWYZ9gSA8JSkKrx0dXnk3K5VWZBwGAaMhmBwclAjPWUl396U74+zTAxdJ4TFztNhBooyySmXs1iUNOO5LFY9S2C3Je8uFAJTVwtCvKbFh9tJpzXojo1QZraJZVs6ZMV/VWz+Azp80i+SNcszkC5fNzNltqNpaYc+uNbxkGt3El9IdRnOcghP457la3p2wi64PdSO0C/D+kX/FLjG4EvaVvzqQ/KkuJGXUOcP136SR/Kk+gvjm2gB+UpB+rzHLWt/qEd+Z0EWgMJIvqYdGxRbtrzTCC3FPSUkL5ta3ftHINJfSHmcp7pw4hS2zhnba3p96E4KFygMEBrF1XBBscg1cTTRCIvX3BVNqa2hRJ53K8JdCJaXEk1n8Jq2tGkVtKikcOk1wLdFvigH/7RokDSIo5c6nXVL6ENqG8hZXFTm21+75OlIK92QxjS5zlapFnkahdnyg4dw30gr2ZjoW38ovFPvVxn/KG8WrkwiS9E22alsR9R2Vq9apHcts07h/ZrdUAmskltczOSz70hUD6Oac5LMnQ/pq4+07yKqbQG6B42aK2L6xPgN8ZIrW2TjK25mGfA2sAcqtWdsl3jZMgcg+t9niGvay+blMutMvMSDda+1AYTNy/LRGIxY9BHYlndixtkDpOZsrWfGEUpaImLOClS2w56RjklvRXQtW0UQDta7CWLPC2w+I+8ms1NSE4ehEfQB59QjgWvjT5Z8oFk5GMgbhRD+7yExmjt4tm2CcxmrLfJ7IIPJNgaxyH9HANiycU3NfJlw1BdtAHlSF5EZWsD91okV+MgB19wvyvFsRWRbNK0Pgj2IRmkPHiL+jAHNKe75p3mlfo2HytCcmlDAVFuP8ch6wfIHrXfx1zMM+wYEogpGG5ba2GSuTvUF0L9kCN5EZWd9jwrtTUNpi/RXBcwUNsW3hLxqVzXxIS4cNWgsl2BTgltLtGOqCd6RIa0owWXlC8Ymox9ixoLuRYCySJRh6Trms0Nol/7FkfKsPm0lauRpfvRfjYw8rA91hva5znyx5BP7ZNsAn1MKlPjLu/bUuqTP4kvhPohh3FIfqnPOE6ToXLxR63PKYfsko0lNBcYt7OBvii1K320BqlcPj8iktPnH5XTOECefMvaQr7arWWSsTXteUabyKxd90D2djFUz+t9nmnsTRv/JI2p4sUXX0zHUaBx2W+U5Zw8eTIdR5M0HYE/n19S+F8Q4q+1r169enxWx6233jo++5gPPvggHUeT/OJPZ0BsN7bDTz9wj58ziD/nEGXffPPN47OP5Dz00EPpfO/evelHRvUzEfEnAsgbLXrNvffem67xG4wmcqrz+uuvp+vRYle0o4T8GvtAbQvsOHr0aLL/qaeeSuX0MxCRGlnoloNNwE+PxHEwpM9z+HmHdevWNQcOHEg+JXFd89Mmf/nLX8ZnVxd+nkXoh2djwi/0PefxJyZyZuOLLu666650fOONN9IR/vSnP6Xjbbfdlo7XA3EuRvgJkPxnbRh/zAVYs2ZNOtaS/0TILbfcko75nHj55ZfTUb8lp9+Xu3DhQvPSSy+l80iUGdcn1p5cf8H8ZRzE+fbhhx82Tz75ZBpTtVwL84y27rvvvuaZZ565uO7Fn/Eqgc9Yd7773e+Oc2aP51kHo0XKLED41MInqxr41MMnKYZLzSdunlBRNn5q49NYabjpNSyp7ZN1G/FTM3X55Mk5efmnSj3hQjcRdUKP+GlRn/hieeTLD1E+dWM50Kc42YSvpSdJeub1upBf1QfIIU/9GJ+S8roF1Fbeb32yQDbolZn8HeULyavp8wj+xqe5PK5jf5RAfu0YrgU78rED2KG28AM+lW6Mi9x3OW1yI7PxhdAYQKccdCDR19K5Zj4PReOEdmpgzMRx0wUyNQflD83V/A2A8kv3ukAuddRf8lUuB11K8lWWY47WoThW6F/y8FtEsmPZOAdrfQazGVvTmmdaY2Jb2BPnVcku7uev7Usgu2+OwWx8Ia6FeYafkD1tHBQuUOJk7INyWohI+eIViQtxnvL2GNTxfs2EzmEiK8BiQjP5uI6LNPpyL7alJJ00wZRPefSJG1vuh5ii7lrcSKXFQBuDUpc/RZdf0T1ulqUUF8A+WRF0lVwFlxzjYjikz3OQly/OgvyS/4T6fhqgp8YRKV/Ioy3Yzrn8UhorEe5JbputMFtfxPGJTlzHDY5zleE+8tp0ng2MoSH9Qvl83HWBHflcJWiIYzLOQdIQffL+JdFeHlhGf3MO+DOOo9hnXesQKUf5cX2QXbQxpO9mO7aG+K8N2pBNMcl3si2OBfqavOiDHOpHn+dzN2e2voj9fjXnGW1Msmf2sYh/RsqbBQaP5B999NGp/C8QxlwNeD34yU9+svntb397yas4c3Xhqwpf+cpXOl+TR/TatLa8ubJ4nl170Cc33nhjMwqWp94n/k7hAoWBdD0EhLX/JdB8AJtKtsbvOpqP4TtW3/ve99KHG3NtwHeE+T7dAw88MM7ph2DwagSEBKOl+db23b6FiufZtcfjjz/ebN68eU6CdD8pNMZc1/BHBsCXxc3VQ39E8MILL6Q/tjHzC8+zawP+8Oixxx5LD3XiHx9NCz8pNMZc17BJ3XPPPX6iehXZv39/SseOHXNAOE/xPLv6EJj/8Y9/nLOAEPyk0BhjjDHG+EmhMcYYY4xxUGiMMcYYY0Y4KDTGGGOMMQ4KjTHGGGOMg0JjjDHGGDPCQaExxhhjjHFQaIwxxhhjHBQaY4wxxpgRDgqNMcYYY4yDQmOMMcYY46DQGGOMMcaMcFBojDHGGGMcFBpjjDHGGAeFxhhjjDFmhINCY4wxxhjjoNAYY4wxxjgoNMYYY4wxIxwUGmOMMcYYB4XGGGOMMcZBoTFmnrJp06Zmz5494ytjjDF9OCg0xsw7du3a1fy///f/xlfGGGNqcFBozBXixIkTzZYtW5rTp0+Pc8xccPjw4eaGG25oPvvZz45zPoJ8/E8/GGOMuRwHhQsUNkZerw3l/Pnz6ZXc8uXLm0WLFqW0atWqtOEiU3lt6eDBg2NJ7aBXrBOh/SVLlly8hx4ibz/aF+9RHzki5s8V2P3tb3+7eeKJJ5oVK1aMcycDX9f03dmzZ5vt27df9Be+om6Ea/pPPqB89M1cgHyCs1wXwfiSzugWg+i2MSZ/YPOvfvWrZseOHeka9Ar5c5/7XPOTn/wk9UPNOIxMOl9y8j6R7uSjZ7SplChXA/ri41g392XenvwUafM3Yykvn5ct+Ssvk8vIdarppxo78jJKWrsiNfJEjZ/bfKg0l/MNPRhr6BAhP+pNX0WdS+Anrfsch84hc50wYxYkx48fn9m4ceP4qo5Tp07NLF68eIZhs3v37plz584lOVwfOHBgZt++fek+5ZRPotyhQ4fSOff6iHVJZ86cGd+Zmdm2bdsl93J5ur9s2bLUbgR7VQ99BbaUZAl0R24J6vT5EXvwS67PUKi/efPmpGtN31GOPgHqrly58hI95GeVwc+UabMVZusL6msM0X4OfaExJHuH+E59GVOuE7LURi3oWuPzLhhz6EPbGn/SF99jK/5HP+VzDTt37kz1atAcoK58rLZzPzJPyKftNvIxh67oQl6cR9A1/wRyKEOKYwm5yid16ZRTY4d0xregNYkU1xiokTeJn9U20KdtY2q28wzQAd1oN8418vEF8jkncY6ObVAfOfm4jXLN/MBB4QKFyTxkk2Ph0MLGYhYhj4WK4EIBRr6paVGpgQWRttSeFh7J0KZSWrB1L9cRtEBKhkDXLl9occ0XaWyOG3wbyJZfZgP20mafvm1oE5Q/sSeXozLYXGI2vuCeNsWoR4Q+j32HXMr2+bgEbam9nK4NuQS6TuJzITtI+FggF78BPpVPaIuy8kVtnzPOqIfMPNApUdIpR7pEX5byINe7BONH5aJNnCufhM9qUZ0uO1QmjrtSHii/Td4QPzNnJK/WptmuOUBd6Rntoy55EenYZi+y0CeCHrl+5vrHQeEChUUiLsh9sPizaLAQtAUMES3uXZtDG9QhAJIMLWgEDOiBDuSXAi3ySfnipkWPRUxltJjTTr655eSLdO3irKcfNRt0Leg6pO+ENgP1HzJyOfiaMvkmGZnUF5FSG/JV3nfk9fVPDjrm4yeCztyr7RdkTOJzIV1qZVCW1BXk5GCz5kaNv9TXJI2JHPJLuqidvM9LZSOSp2BF/kAOMtGbfM5rqbEDffIyGgOkOA765JE3xM9qWzZpvvRBO5POM8roQzNtxzkgH+d02ZN/WAP6bjZzwlyb+DuFpornnnsuHfkeytKlS9N5F0ePHk3HNWvWpOMQjh071qxbt2581TQnT568+L2e0SLUXLhwIZ3fdddd6Sji92ZWr149PvuIN954Ix0feeSRdISXXnopfZ8HXe++++5xbhlsPnLkSPPWW28lH6Df3r1703kXb775Zjreeuut6Xi1wM7vf//7zWgzvth/X/rSl5Lt8htlfve736XzLib1RR//+7//m45/+7d/m46Rl19+eXxWh3QcrXHN2rVrx7kfo+91/s///E86ziUaY/C1r30tHbvoGsddMMY1N7785S+nYxfMKxgFHq1zWvMG0EXfiaSdUbBySZ/X6I28UWDTfPrTn07X+AX/IJMx9M4776T8DRs2pGMNNXa89tpr6agyfH/u61//esobBVCXzM8+eUP9rLapw/fxPvOZz1TZpzE8dJ7RR4899lj6/nIJ1lCI35Xs+37gKGhOf7yVo3Ft5g8OChcI+Zen//7v/z5N6JhHauPtt99Ox3vuuScdu5h0UwM2CNoi4Fu/fn3Ke//995uf/exnzdNPP928/vrrKW/0yfWyP9joWsxZmFkMyWczg6eeeuripnfbbbelYxfU/bd/+7fm2Wefbe68886qIOiPf/zjxUU4J/d9KXV9yX0I//RP/5Q2oq1bt45zmnQ++vSfxgJtxT8M6PPHJL6YDXP18zL/9V//NT67lNnOl8gf/vCH8VmTfNVHTZBTQsEHc0NBTvwjolxfBdpdAYpkwo033phkE0AcP348BSiR2uCM9mKg/vjjj18cQ3wghC9+8YvpWEONHZLL2oIfCMyAYCcfu33yhvpZbe/evTt9SGHOxQ+9XUwyzzZv3pzWtrwPGNOszaybBMKsqejKH42wTsHtt9+ejrUQ4Jt5RnpeaBYcvE6offQfX6fE1xBt6PXEaHMY59Sj10ggOSS9CuHItV6pRLCHe6VX1rz+0OvmaA9yavXkdQ5y0KH2NQ421Pq5lqEysbH0qr0E5bCxj0l8EcH3+VjS67z81SN52DxthsgdMl9y4jiuoWscd6F6Uc/42jT3t/LbXvUCc4MyjCH1Odcl3Wr0jvNQ7ZPo+7bXuX2oTpsd6K0yjNPYTqlO1z2Y1M95fg1D5xm+VXul1KaDfNLmd9rO+xX7ow/M/MBPCk0vN9100/jsUnhiUPrZCb1q7vrk3sarr7568dOwPrWOFqSLr0L0qbv0xFKvMvJX1rxO4YmAXjfzlGK00KbzJ598skpPnmBiK2Wx+5VXXkmvvGp+loG6Vwt0vOWWWy4+Idy/f/8lT3Ij6Pnzn/+8efTRR8c5ZWbjiy54gkFfx6dT+gmWoU8wriVuvvnm8dml4LP8STC+bRvHfZS+ovDBBx+kI36NT+fanubz1Ej38L3eEHz+859PT56+8Y1vpGvmTU5J72ij5uG9996brnmiCKOgOfV9fAtQ+3WLGjv4moigbdoaBTPpmp8vitTIm9TPMZ/50/cWYJJ5xjwf7euXJBgFg+k86hD56U9/mt6gtPkdHbT2ivfee6+54447xldm3jAaKGYBwifGIZ/yKMtwiU/bSp8e46fwoU86+FQcZdJGbDN+Cs4/xcdP6vmnXT2pQb7okpVDPZ6Y5E8nsRV9uz69c482pgn+4elBjvpIYGOuM2VKTwvwQcnGnNn4QiADPUtlsQ05yKMc7dDeXFDT92LofIlgBzbRHnaBxl/e/pBxmaP5wtigTeYB5+Tl/YWfyY++pQx5Ij7hRB6oDVLUj/O8rMa+xls+D/GndCVJ1yH9XWOH5kUsI13ol7he1MibxM9x7OR+KYFcdMjlDZlngBzaaiuPPJ5C0hZlhfwjHdW/ksM4zX1n5gfT3a3MdQOTfcgmx4KhV7dK1M8XtnifNGTR0OJNigsUaHFTirrHRZnEgia0gJPiAhvl5W3lsCDmi7NgUe3yI7rRxjQWTzYYFm/pjc2xbfkPaE+BSJ7yPqMccms2mtn4QvejXlzHdukL5Os+Y24avsvRuKiVPXS+5GB77Dt8kNsWxyqJ/h0KvtRcoA3GDNcxgKOdtrEhG3Ndou2xLmBDtC1PEOWhU46CJyXK91FjRwxsSVob6I+8bI08MVs/k7qYzTyLRB2pF2H8cR+f5GtgHhQCebKH/q7pI3P9sYh/Rp1sFhi81uA1IX/dZuYWXgHxRzPxf9kwVxf+b+Tf//731ePf88UYsxBwUGiuKGyu/CVnzuiT77zdcLH5C1/4QvPuu+8O+mtSMzfw3baVK1em72jlf8F+rcP30B566KHx1cfs3r173n7oyP+aV3jrMmb6OCg05grAl8P57TACXweGVw99ef873/nOnP+MjjHGXG84KDTmCsETw1/+8pfNww8/fN09oZoPyP8PPvhg619hGmPMQsZBoTHGGGOM8f9oYowxxhhjHBQaY4wxxpgRDgqNMcYYY4yDQmOMMcYY46DQGGOMMcaMcFBojDHGGGMcFBpjjDHGGAeFxhhjjDFmhINCY4wxxhjjoNAYY4wxxjgoNMYYY4wxIxwUGmOMMcYYB4XGGGOMMcZBoTHGGGOMGeGg0BhjjDHGOCg0xhhjjDEOCo0xxhhjzAgHhcYYY4wxxkGhMcYYY4xxUGiMMcYYY0Y4KDTGGHNNc/bs2Wb79u3Nnj17xjnGmLnAQaExxphrltOnTzfPP/988+GHH45zjDFzhYNCY4y5Apw/fz496dq1a9c458pw8ODB5sSJE+Or648VK1Y0O3bsaD772c+Ocz5my5Ytyaf41hgzexwULlDYJDZt2jS+mhzksDAvWrQopSVLlqRrQL7yS2n58uWpXA15O6RVq1alpwiCzSHeL71q0sZM27Fs3Kh5TRXvxSTbAJ3ivby9XB825xpKOuLXPJiIskkl4v0YGMS+kQ9ze/CD8mmfPI59RDmUjxt2zM+hHm3GtrjON/xcTyX8lfdBXnZafTQUbMDnN998c/PjH/94nPsx9AH2om8NyGP899lL+sd//Mfx3bnj8OHDresJtmm8YWM+jtvWiRpf0F833HBDkpGPE2PMBMyYBcnx48dnNm7cOL6ajG3bts0whFauXJnkATKXLVuWzhcvXpzKAPmU3blzZ7qmzubNm9N5H6V2Dhw4kPLOnTuXrgVtk98mW3rs3r07XVMfuboGrikn2Wofe/L2JI8kW+HMmTMX80m1tp46dSq1Qx10oj3pKN8J+YBEvRLIUJmoH34kb9++feOcj6Ad8vFjtFX5be3kRL+gp5A+uRzZgu3qY5VFVg7+jPfwt/wW24Np9BEy2mxH5qFDh8ZXZagfx1hE/YsOsr0Pjclcpvp1CLOxDd3zvohwP64D0q/NF11Qp60e+bVzzBjTjoPCBQqLc2kRr4VggsWdBZ/NVWhxZjNAvgILypK0wXRtkpG2dtrI24nEIKBr8+W+2uoLvBRAcj/6k3Plt9XNwVcKavMAsAT+oyw6tIEc6YAP1R9twYOCk9g31CEv2teH5OT1kJvLkS6k2G8xX3oL+TbqWcqDafQR4wD/5WUIdpCf6xfBDuq2gQyN865xKdCFOYbuua1t/drFbGxDD+qV+hU0f6IM6uiD4xCo1zYvNEZr/GeMacdB4QKFxbO0iNfAAswmwiKcb0oltFHlm0Mfc9GO5LHZ9QVq3Je8/IkaaCPShi5/apNFZ/I5ryGWr/GTApquABI72dwpR0I3KG3iMWiOviFQI6+mD0B+ie0qyKbNXI7syPWJ/Rk3e8knxSBSfSsbYZp9pDryTU3QBJQjlUAmwQ6gR7SzBG2rzZIv5bOhTGqbKI0nkJyIfF5LHAdKJfBjm5+NMXX4O4VmMG+88UZz4cKFdP7lL385Hbs4efJkOo42h2bp0qXpvIa5aGfv3r3p+Pbbbzef+cxnWr9DxveT7rvvvnQ+2myarVu3pvMI+o020ubTn/50uj569Giqx/fgaOedd95J+Rs2bEjHPp577rl05HuLNX6iPVizZk065qALdt5///3JJ/Cb3/wmHWlr/fr16Vy8+eab6YhNfLlfvPbaa+l49913p2Mf+AUeeeSRdISXXnop6YPOUQ4/NSI7vva1r6Wj+O///u/xWdOsXbt2fPaxfFi9evXFnythrIyCgku+9znNPkIuddatW5fO33rrrebIkSO9fXXs2LHmlltuGV99DHo/9thjzRNPPDHO6efrX/9689RTT7W2+Td/8zfJXr5vyPfy+A5f/r3DEpPa1gc2tsmo/f4kfT/aqy5JJfhDFPQ2xsyC0QQzCwB9Ou9LNUhW7SsgPQmqeSUa0avP2A5PHaQrKVLbjp62SEZ8KiZ46sA9yrU9LaGd+JRHbeuJCU9eyItPrtrQE61YXk/olOJTofj0pE0/1QdkqryeCManbMBTFvJlk5Cvaok+kExkSJ+ob7RRTxOF+iAfZxoXMeHr0lO2afaR0Djre6on2srikzj2VI5+brMlPrFGD5WNYyOiJ6S19g21TdC+fBohL8+nLG2U5t1skFxjzOR4Bi1QWPRLi3gN2jhq61OWlAchfZTaiUFEvnENaSe+Ks03VG0uBAxdGxfBijZpySJRh6TrPNgpgS0qH+1SQJb7Wjpyvw0CMgVEBGIKgBSo5cGk2oqBh4LV2r6G6JdoF+3m+soOUkTtkvL+kZ7IoxztcU3QlDPNPgJk4Uf8ih65D0sgPx+ryFHbpZSXj7qWUu6jCHrmgX6JSWwTtF8aI5IVUZ9Pm7mSa8xCwq+PzWBuvfXW8dml8JMg+SshfqpC8KpvCKV2Pvjgg3QcbV6XvFKM7cZ2pBOv0OJPZkTZ/EyIoOxDDz2UznmdxmtUXoHxKo7XjoK8USDR3Hvvvel6tPGl42hjSnVef/31dD0KSlr9FbnpppvGZ5dCG/ClL30pHcXLL7+cjvG1J6/Co428tuR1IPAKT69Vn3zyyeIrdl41g161Aq99IW+/DfnlrrvuStf0ET4A2s1f00bfRx5//PF0pJ8feOCBdA7Il56f//znkw3f+MY30jXyI9PuI/z78MMPN6+88ko65xVt7U+h/PWvfx2ffQRfRxitv5ckGAWD6TyObUDfvPwoCEu2cM7v+LWhr2B0MRvbumD80V9Rzvvvv3+xL4wx1xijBcUsQHgSMeTpT0RPf3gKw9MEUtvTJ54UkF+610feDk9zOCeP9iJ6pRifSkgnQA+egvDEBfSkJpZHPmVy+V1PO2QTvoz+kJ55vS6QQR092VIbJHQTyFe+nopiF7pTB+S7+NQ0Pm0qPVVT+7onf8uuGnK/QHwqlj/FpZx8rr6RjNhfIvpEbchWUpSf6zKbPuL1a0kfxgn11UYJ2lG/tEF9dImveWVXW13azecBNlJe+sj3yGpjNrYJxgx25mhOSU/04Lrmaf5QsDv2Jb4gGWPqcVC4QGFxns2CyUaiTZXEeb5BaVNWyu/XENthM9HmEzcVbTSxLSXZqA1D+dqo4oZH2Vg3pqi7NmtSKbgiL9bt2pAj6BKDaPThOu+nWCZPakvX+UYtH5Q2ZdrHTtWVj2JA2kX0S/QXcpVfCjAIRqJNGkt5u1E+Kfol9j9Mu49oKw+aBLp2BTncj/4oEce42pENpaAQedI9BpL5OOe8LwCbjW34MbaHHfl4pb7s46gPPdOGdqOfuWZcGWPqWcQ/o8lqFhi8Jn300UfTXxgaY+YO5toXvvCF5t133531X/OaMryevvHGG9Prd7165xX4j370o+Zzn/tcujbG9OPvFC5QWDivhYCQ7y3F/9pKKX5HcL6ATSVb4/cArwcWUp9NA+banXfe2fz7v//7OMdMG76Dunnz5osBId+L/M53vuOA0JiB+EmhMcbMMTzJIpgmUNEf/JjpQADI7z1O43cVjVno+EmhMcbMMQQrBC1//OMfm127do1zzWzhR8jxqQNCY6aDnxQaY4wxxhg/KTTGGGOMMQ4KjTHGGGPMCAeFxhhjjDHGQaExxhhjjGma/x85xRLL7eSBTQAAAABJRU5ErkJggg==\" width=\"645\" height=\"270\"\u003e\u003c/p\u003e\u003cp\u003ewhere \u003cb\u003eMORPH_AV\u003c/b\u003e represents Surface To Volume Ratio, \u003cb\u003eIH_SKEW\u003c/b\u003e represents Intensity Histogram Skewness, \u003cb\u003eIH_P10\u003c/b\u003e represents Intensity Histogram 10th percentile, \u003cb\u003eIH_energy\u003c/b\u003e represents Intensity Histogram Energy, \u003cb\u003eGLCM_NIDM\u003c/b\u003e represents GLCM Normalized Inverse Difference Moment, \u003cb\u003eNGTDM_STR\u003c/b\u003e represents NGTDM strength, \u003cb\u003eGLSZM_LZLGE\u003c/b\u003e represents GLSZM Large Zone Low Grey Level Emphasis, \u003cb\u003eSKEW\u003c/b\u003e represents Intensity-based Skewness, \u003cb\u003eMIN\u003c/b\u003e represents Minimum Grey Level, \u003cb\u003eGLRLM_LRLGE\u003c/b\u003e represents GLRLM Long Run Low Grey Level Emphasis, and \u003cb\u003eGLSZM_GLNU_NORM\u003c/b\u003e represents GLSZM Normalized Grey Level Non-uniformity. '\u003cb\u003ePET_\u003c/b\u003e' and '\u003cb\u003eCT_\u003c/b\u003e' prefixes indicate factors derived from PET and CT images, respectively. The composite Rad-score demonstrated significant prognostic value with a C-index of 0.671.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003ePrognostic Significance of Clinical Variables and Radiomics Score\u003c/h2\u003e \u003cp\u003eUnivariate Cox proportional hazards regression analysis to assess the prognostic value of clinical parameters and the Rad-score (Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e) indicated that significant predictors of OS in patients with PDAC were age (HR, 1.040; 95% CI, 1.016\u0026ndash;1.064; P\u0026thinsp;=\u0026thinsp;0.0027), serum haemoglobin (Hb) level (HR, 0.840; 95% CI, 0.750\u0026ndash;0.94; P\u0026thinsp;\u0026lt;\u0026thinsp;0.0001), alkaline phosphatase (ALP) level (HR, 1. 001; 95% CI, 1.000\u0026ndash;1.001; P\u0026thinsp;=\u0026thinsp;0.0139), and carcinoembryonic antigen (CEA) level (HR, 1.021; 95% CI, 1.012\u0026ndash;1.031; P\u0026thinsp;\u0026lt;\u0026thinsp;0.0001). Smoking history showed borderline significance (HR, 1.510; 95% CI, 0.942\u0026ndash;2.420; P\u0026thinsp;=\u0026thinsp;0.0869) in predicting survival. T stage (4 vs. \u0026lt;4), N stage, and other laboratory test results had no significant prognostic impact. Notably, the Rad-score outperformed all clinical parameters with a higher hazard ratio (HR,12.698; 95% CI, 5.743\u0026ndash;28.074; P\u0026thinsp;\u0026lt;\u0026thinsp;0.0001). In Kaplan-Meier curve analysis with log-rank test using an optimal cutoff that produced the highest chi-square of Log-rank test, patients with higher and lower Rad-score demonstrated significant differences in overall survival (median OS 10.8 [95% CI 8.4\u0026ndash;14.2] vs. 27.1 [19.6\u0026ndash;34.1] months; chi-square\u0026thinsp;=\u0026thinsp;29.254; P\u0026thinsp;\u0026lt;\u0026thinsp;0.001; Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eUnivariate survival analysis using clinical parameters and radiomics score. N+, presence of regional lymph node metastasis; WBC, white blood cell; AST, aspartate transaminase; ALT, alanine transferase; ALP, alkaline phosphatase; CEA, carcinoembryonic antigen; CA19-9, carbohydrate antigen 19\u0026thinsp;\u0026minus;\u0026thinsp;9\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"3\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eFactor\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eHazard ratio [95% CI]\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003e\u003cem\u003eP\u003c/em\u003e\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAge\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.040 [1.016\u0026ndash;1.064]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.001\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eSex\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.022 [0.678\u0026ndash;1.541]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.918\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eSmoking history\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.510 [0.942\u0026ndash;2.420]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.087\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAlcohol intake\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.021 [0.673\u0026ndash;1.549]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.922\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eT4 (vs. T1-3)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.283 [0.850\u0026ndash;1.937]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.235\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eN+\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.818 [0.508\u0026ndash;1.319]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.411\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eWBC\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.070 [0.991\u0026ndash;1.154]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.082\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eHaemoglobin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.840 [0.750\u0026ndash;0.941]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.003\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePlatelet\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.002 [0.999\u0026ndash;1.004]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.207\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAST\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.000 [0.999\u0026ndash;1.001]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.551\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eALT\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.000 [0.999\u0026ndash;1.001]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.774\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eALP\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.001 [1.000\u0026ndash;1.001]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.014\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eTotal bilirubin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.010 [0.988\u0026ndash;1.033]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.369\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eCEA\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.021 [1.012\u0026ndash;1.031]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;0.001\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eCA19-9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.000 [1.000\u0026ndash;1.000]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.175\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eRad-score\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e12.698 [5.743\u0026ndash;28.074]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;0.001\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003eN+, presence of regional lymph node metastasis; WBC, white blood cell; AST, aspartate transaminase; ALT, alanine transferase; ALP, alkaline phosphatase; CEA, carcinoembryonic antigen; CA19-9, carbohydrate antigen 19-9\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec12\" class=\"Section2\"\u003e \u003ch2\u003eAdditive Value of the Radiomics Score to Clinical Prognostics\u003c/h2\u003e \u003cp\u003eMultivariate Cox proportional hazards regression analysis compared the prognostic value of clinical and combined (clinical\u0026thinsp;+\u0026thinsp;Rad-score) survival prediction models (Table\u0026nbsp;\u003cspan refid=\"Tab3\" class=\"InternalRef\"\u003e3\u003c/span\u003e). In the clinical model, age, smoking history, serum ALP level, CEA level, and T stage were identified as independent predictors of OS. In the clinical\u0026thinsp;+\u0026thinsp;Rad-score model, Rad-score was as a significant prognostic factor (HR, 12.793; 95% CI, 5.54\u0026ndash;29.546; P\u0026thinsp;\u0026lt;\u0026thinsp;0.0001). Thus, Rad-score was included in the model and T stage was excluded. Inclusion of Rad-score in the clinical model improved prognostic accuracy as evidenced by a higher Harrell's C-index (0.740 vs. 0.673). Bootstrapping analysis showed that the difference in prognostic value between the clinical and clinical\u0026thinsp;+\u0026thinsp;Rad-score models was statistically significant (difference in C-index, 0.069; 95% CI, 0.014\u0026ndash;0.113; P\u0026thinsp;\u0026lt;\u0026thinsp;0.0001).\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab3\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eMultivariate survival analysis using clinical parameters and radiomics score.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"5\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003eFactor\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"2\" nameend=\"c3\" namest=\"c2\"\u003e \u003cp\u003eClinical model\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"2\" nameend=\"c5\" namest=\"c4\"\u003e \u003cp\u003eCombined model\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eHazard ratio [95% CI]\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003e\u003cem\u003eP\u003c/em\u003e\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eHazard ratio [95% CI]\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u003cem\u003eP\u003c/em\u003e\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eAge\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.050 [1.025\u0026ndash;1.077]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;0.001\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1.052 [1.026\u0026ndash;1.078]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;0.001\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eSmoking history\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e2.067 [1.242\u0026ndash;3.440]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.005\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2.080 [1.237\u0026ndash;3.498]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.006\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eT4 (vs. T1-3)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.454 [0.940\u0026ndash;2.251]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.093\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003eN/S\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eN+\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003eN/S\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003eN/S\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eHaemoglobin\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003eN/S\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003eN/S\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eALP\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.001 [1.000\u0026ndash;1.001]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.043\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1.001 [1.000\u0026ndash;1.001]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.034\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eCEA\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.021 [1.011\u0026ndash;1.031]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;0.001\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1.014 [1.004\u0026ndash;1.025]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.007\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eCA19-9\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003eN/S\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003eN/S\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eRad-score\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e12.793 [5.540\u0026ndash;29.546]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;0.001\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eC-index\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c3\" namest=\"c2\"\u003e \u003cp\u003e0.673 [0.650\u0026ndash;0.766]\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c5\" namest=\"c4\"\u003e \u003cp\u003e0.740 [0.715\u0026ndash;0.816]\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eC-index difference*\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colspan=\"4\" nameend=\"c5\" namest=\"c2\"\u003e \u003cp\u003e0.067 [0.014\u0026ndash;0.113]\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003ctfoot\u003e \u003ctr\u003e\u003ctd colspan=\"5\"\u003e*Difference between two models produced by bootstrapping 1,000 resamples.\u003c/td\u003e\u003c/tr\u003e \u003ctr\u003e\u003ctd colspan=\"5\"\u003eN/S, not significant; ALP, alkaline phosphatase; CEA, carcinoembryonic antigen; CA19-9, carbohydrate antigen 19\u0026thinsp;\u0026minus;\u0026thinsp;9\u003c/td\u003e\u003c/tr\u003e \u003c/tfoot\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e"},{"header":"Discussion","content":"\u003cp\u003eWe successfully formulated a prognostic model for the evaluation of patients with PDAC using FDG-PET/CT-based radiomics. By identifying key features through texture analysis of FDG-PET/CT images and integrating them into a composite Rad-score, we constructed a model with a nuanced approach to oncologic prognostication. The Rad-score was validated, demonstrating statistically significant, albeit moderate, prognostic ability to discriminate between survivors and decedents, indicating its potential utility in clinical prognostication. A combined model, incorporating both clinical parameters and Rad-score significantly outperformed the use of clinical parameters alone for predicting OS in patients with PDAC, indicating that Rad-score may be a crucial biomarker for survival outcomes. In addition, multivariate Cox proportional hazards regression analysis demonstrated that integrating the Rad-score with clinical variables significantly enhanced the prognostic accuracy of OS prediction models. This study underscores the critical contribution of radiomics in refining the accuracy of survival prediction and its potential in guiding personalised treatment in oncology.\u003c/p\u003e \u003cp\u003eSeveral studies have demonstrated the prognostic value of radiomics in pancreatic cancer, mainly based on contrast-enhanced CT or MRI\u003csup\u003e\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e\u003c/sup\u003e. However, research focusing on FDG PET-based radiomics for predicting OS in pancreatic cancer remains limited. Toyama et al. showed the prognostic value of GLZLM grey-level non-uniformity in FDG-PET using a random forest classifier, but this study only estimated survival outcomes over a one-year observation period\u003csup\u003e\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e\u003c/sup\u003e. Hyun et al. analysed a cohort of 137 PDAC patients and reported that first-order entropy on initial FDG-PET independently correlates with survival, as determined through multivariate Cox regression analysis\u003csup\u003e\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e. Similarly, JW Lee et al. demonstrated the prognostic significance of first-order entropy and developed and reported a scoring system that incorporates total lesion glycolysis and bone marrow uptake to predict OS\u003csup\u003e\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e\u003c/sup\u003e. Conversely, Yoo et al., in a study of patients with pancreatic cancer undergoing curative surgery, failed to identify independent predictive factors from heterogeneous features measured on FDG-PET through multivariate analysis\u003csup\u003e\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e\u003c/sup\u003e. Despite providing us with valuable insights, the previous studies are somewhat limited by their focus on individual radiomics features. These studies used conventional Cox regression analysis, which has inherent shortcomings such multicollinearity, ineffective variable selection, and potential overfitting. Addressing these issues, we employed LASSO for robust variable selection and designed an integrated prognosis model to emphasize the power of integrated radiomics data. Nonetheless, we highlight the need for ongoing optimisation to improve the predictive performance of the model. Additionally, we investigated the additive value of radiomics data by comparing it with a prognosis model based on clinical variables alone, further validating our approach through internal bootstrapping analysis.\u003c/p\u003e \u003cp\u003eIn our study, the Rad-score emerged as an independent prognostic indicator in the multivariate analysis, while T stage did not retain its prognostic significance within the radiomics-enhanced model (clinical\u0026thinsp;+\u0026thinsp;Rad-score model). This change suggests the potential of the radiomics model to serve as a more comprehensive and robust prognostic tool by capturing the intricate characteristics of primary tumors, which may not be fully appreciated through traditional clinical markers alone. Additionally, while certain clinical prognostic factors such as age, smoking history, and carcinoembryonic antigen (CEA) levels aligned with findings from previous studies, other factors like N stage and carbohydrate antigen 19\u0026thinsp;\u0026minus;\u0026thinsp;9 (CA19-9) did not\u003csup\u003e\u003cspan additionalcitationids=\"CR15 CR16 CR17\" citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e\u003c/sup\u003e. This discrepancy may be attributed to the heterogeneous disease status and the variability in treatment and follow-up strategies among patients involved in the study. The need for further validation in a large multi-center cohort with unified management strategies remains to solidify its usefullness in clinical practice.\u003c/p\u003e \u003cp\u003eThis study has several limitations. First, its retrospective design has an inherent risk of bias because it includes patients who received different treatment regimens, several of whom received follow-up treatment after initial diagnosis at hospitals other than SNM-SNU BMC. However, given the strict regulation of cancer treatment by the Korean national insurance system, it is plausible that most patients received standardised treatment in accordance with national cancer treatment guidelines (references). Second, while this study included a larger number of patients compared with previous studies, its single-centre design may limit the generalizability of the results. Prospective multi-centre studies with external data are warranted. Third, lesions were excluded from analysis if there was insufficient tumour size for evaluating FDG distribution, PET-CT misregistration due to respiratory motion, or challenges in delineating tumour uptake on FDG-PET/CT images. This exclusion criteria are a common challenge in PET radiomics analyses, because it safeguards appropriate texture analysis\u003csup\u003e\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eAn FDG-PET/CT-based radiomics model showed potential in enhancing the prediction of survival outcomes among patients with PDAC, and outperformed a model based on clinical data alone demonstrating its potential applicability in the field. Further prospective studies with larger cohorts are warranted to validate the results of the current study and establish the model\u0026rsquo;s applicability in patient management.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare no conflict of interest.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting Interests Statement\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare no competing interests.\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eY.K., S.H., J.B.J., and S.W.O. designed and conceptualized the study. Y.K. and S.H. collected data, and performed radiomics analysis and statistical analysis. J.B.J. and S.W.O. critically discussed the analysis and results. Y.K. and S.H. initially wrote the manuscript. J.B.J. and S.W.O. revised the manuscript. All these authors read and approved the final version of the manuscript.\u003c/p\u003e\u003ch2\u003eData Availability\u003c/h2\u003e\u003cp\u003eThe datasets generated or analyzed during the study are available from the corresponding author on reasonable request. Due to patient confidentiality agreements, raw imaging data are not publicly available but can be accessed upon approval by the institutional review board.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eRawla, P., Sunkara, T. \u0026amp; Gaduputi, V. Epidemiology of Pancreatic Cancer: Global Trends, Etiology and Risk Factors. 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Cancer Epidemiology, Biomarkers \u0026amp; Prevention 16, 546\u0026ndash;552, doi:\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1158/1055-9965.Epi-06-0893\u003c/span\u003e\u003cspan address=\"10.1158/1055-9965.Epi-06-0893\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e (2007).\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"FDG-PET/CT, Pancreas cancer, Radiomics, Survival","lastPublishedDoi":"10.21203/rs.3.rs-4825555/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4825555/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003ePancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis even without distant metastases, necessitating in-depth characterization of primary tumours for survival prediction. We assessed the feasibility of using FDG-PET/CT radiomics to predict overall survival (OS) in PDAC. This retrospective study included PDAC patients without distant metastasis who underwent FDG-PET/CT for initial staging. Primary tumours were segmented from FDG-PET/CT images, extracting 222 radiomics features. A radiomics-based risk score (Rad-score) was developed using Cox proportional hazards regression with LASSO to predict OS. The prognostic performance of the Rad-score was compared with a clinical model (demographics, disease stage, laboratory results) using Harrell's concordance index (C-index) and bootstrapping. 140 patients were included, with a mortality rate was 72.9% during follow-up (total population, 19.5\u0026thinsp;\u0026plusmn;\u0026thinsp;19.2 months; survivors, 34.4\u0026thinsp;\u0026plusmn;\u0026thinsp;28.8 months). Eleven radiomics features were significant for survival prediction. The Rad-score predicted OS with a C-index of 0.681 [95% CI, 0.632\u0026ndash;0.731]. A model integrating clinical parameters and Rad-score outperformed the clinical-only model in predicting OS (C-index 0.736 [0.727\u0026ndash;0.812] vs. 0.667 [0.648\u0026ndash;0.750]; C-index difference 0.069 [0.028\u0026ndash;0.117]; P\u0026thinsp;\u0026lt;\u0026thinsp;0.001). These findings suggest that incorporating FDG-PET/CT radiomics into preexisting prognotic stratification paradiagm may enhance survival prediction in PDAC, warranting large-scale studies to confirm its applicability in clinical practice.\u003c/p\u003e","manuscriptTitle":"The Value of PET/CT Radiomics for Predicting Survival Outcomes in Patients with Pancreatic Ductal Adenocarcinoma","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-09-12 12:27:38","doi":"10.21203/rs.3.rs-4825555/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-09-04T04:33:30+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-08-29T12:42:34+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-08-22T02:22:21+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"49180887973549519361368070600424272908","date":"2024-08-21T04:14:47+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"192504424273366687147079387230366440683","date":"2024-08-19T08:32:11+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-08-19T02:55:42+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-08-16T21:49:44+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2024-08-09T01:39:01+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-08-07T04:51:36+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2024-07-30T04:23:39+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"5c39ba46-4d3a-4a05-be80-8c2d36dfb394","owner":[],"postedDate":"September 12th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":36785533,"name":"Health sciences/Oncology/Cancer/Gastrointestinal cancer/Pancreatic cancer"},{"id":36785534,"name":"Health sciences/Oncology/Cancer/Cancer imaging"},{"id":36785537,"name":"Health sciences/Biomarkers/Prognostic markers"}],"tags":[],"updatedAt":"2024-11-25T15:59:30+00:00","versionOfRecord":{"articleIdentity":"rs-4825555","link":"https://doi.org/10.1038/s41598-024-77022-4","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2024-11-22 15:56:59","publishedOnDateReadable":"November 22nd, 2024"},"versionCreatedAt":"2024-09-12 12:27:38","video":"","vorDoi":"10.1038/s41598-024-77022-4","vorDoiUrl":"https://doi.org/10.1038/s41598-024-77022-4","workflowStages":[]},"version":"v1","identity":"rs-4825555","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4825555","identity":"rs-4825555","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}