Antibacterial Activity Of Silver Nanoparticle- A prospective study

Antibacterial Activity Of Silver Nanoparticle- A prospective study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Antibacterial Activity Of Silver Nanoparticle- A prospective study Adrita das, Priti Sundar Mohanty This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7390189/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This paper presents the antibacterial activity of Silver nanoparticles. Silver nanoparticles (AgNp) can invade the walls of bacteria cells, altering the composition of cell membranes and potentially causing cell death. Their effectiveness results from their small size (nanoscale) and high surface area compared to the volume ratio. By releasing silver ions, they can cause reactive oxygen species to be produced, which can enhance the permeability of cellular membranes and therefore stop the replication of deoxyribonucleic acid. Silver nanoparticle antibacterial mechanism has been implemented for various therapeutic applications including medical prosthetic devices. In this study, the Salmonella Green Fluorescence Protein (GFP) & Salmonella Typhimurium bacterial strains were encapsulated with different doses of nanoparticle & perceived Microbial Growth, Zone of Inhibition by well diffusion test. Salmonella GFP under Fluorescence & Bright field Microscope concerning Trisodium Citrate (TSC) concentration have been observed. Here, the antibacterial impact of Silver nanoparticles before & after the dialysis process was also contrasted. The result from the Microbial Growth curve & well diffusion test demonstrates that with increasing the dose of AgNp bacterial growth was minimal or antibacterial efficacy became more. However, less Zone of Inhibition after the dialysis process of AgNp solution was observed than before dialysis by which excess salt & reducing agent removes were analyzed. Furthermore, the Salmonella bacterial aggregation in low TSC in contrast, no aggregation has been perceived in terms of high concentration of TSC by investigating with fluorescence & bright field microscope found. All these investigations indicate the antibacterial impact of AgNp. Nanoscience Silver Nanoparticle Fluroscence Trisodium citrate zone of inhibition Salmonella Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7390189","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":501392038,"identity":"a4b57d62-c768-4a2b-8b68-d34e563eeb62","order_by":0,"name":"Adrita das","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA8klEQVRIiWNgGAWjYFCCBIYDDAzMDAzszQcfALk8fERpOQDSwnMs2QCkhY0YLQxgLRI5ZhIgPkEt/O25Dw9/qLGWl29IMKv8mmMnw8bA/PDRDTxaJM48Nzhw4Fi64YYDB9Juy25LBjqMzdg4B581N9KAfmE7zLiBseHYbcltzEAtPGzS+LTIg7X8O2w/v5mxrVhyWz1hLQYgLQfbDic2HGNmY/y47TBhLYZnnjEcONuXnrzhDBuzNOO24zxszAT8Inc8jflDxTdr2/nz33/8+HNbtT0/e/PDx3i9jwyYecAkscpBgPEHKapHwSgYBaNgxAAA+rBOrOm0LaMAAAAASUVORK5CYII=","orcid":"","institution":"KIIT University","correspondingAuthor":true,"prefix":"","firstName":"Adrita","middleName":"","lastName":"das","suffix":""},{"id":501392301,"identity":"cbc55046-1d67-4139-b389-d815132bb199","order_by":1,"name":"Priti Sundar Mohanty","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Priti","middleName":"Sundar","lastName":"Mohanty","suffix":""}],"badges":[],"createdAt":"2025-08-17 03:28:57","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":true,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":true},"doi":"10.21203/rs.3.rs-7390189/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7390189/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":89343979,"identity":"04cb3bf6-7e83-4094-8dfb-4c24dcbd5df5","added_by":"auto","created_at":"2025-08-19 04:09:59","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":916443,"visible":true,"origin":"","legend":"","description":"","filename":"AntibacterialActivityOfSilverNanoparticle.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7390189/v1_covered_aa05c3af-6178-4dc0-83c7-7083cbbc5aef.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eAntibacterial Activity Of Silver Nanoparticle- A prospective study\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"KIIT University","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Silver Nanoparticle, Fluroscence, Trisodium citrate,zone of inhibition, Salmonella","lastPublishedDoi":"10.21203/rs.3.rs-7390189/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7390189/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis paper presents the antibacterial activity of Silver nanoparticles. Silver nanoparticles (AgNp) can invade the walls of bacteria cells, altering the composition of cell membranes and potentially causing cell death. Their effectiveness results from their small size (nanoscale) and high surface area compared to the volume ratio. By releasing silver ions, they can cause reactive oxygen species to be produced, which can enhance the permeability of cellular membranes and therefore stop the replication of deoxyribonucleic acid. Silver nanoparticle antibacterial mechanism has been implemented for various therapeutic applications including medical prosthetic devices. In this study, the Salmonella Green Fluorescence Protein (GFP) \u0026amp; Salmonella Typhimurium bacterial strains were encapsulated with different doses of nanoparticle \u0026amp; perceived Microbial Growth, Zone of Inhibition by well diffusion test. Salmonella GFP under Fluorescence \u0026amp; Bright field Microscope concerning Trisodium Citrate (TSC) concentration have been observed. Here, the antibacterial impact of Silver nanoparticles before \u0026amp; after the dialysis process was also contrasted. The result from the Microbial Growth curve \u0026amp; well diffusion test demonstrates that with increasing the dose of AgNp bacterial growth was minimal or antibacterial efficacy became more. However, less Zone of Inhibition after the dialysis process of AgNp solution was observed than before dialysis by which excess salt \u0026amp; reducing agent removes were analyzed. Furthermore, the Salmonella bacterial aggregation in low TSC in contrast, no aggregation has been perceived in terms of high concentration of TSC by investigating with fluorescence \u0026amp; bright field microscope found. 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