Bioelectricity Generation and System Scaling in Microbial Fuel Cells Driven by Pseudomonas aeruginosa PPNB101

Bioelectricity Generation and System Scaling in Microbial Fuel Cells Driven by Pseudomonas aeruginosa PPNB101 | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Bioelectricity Generation and System Scaling in Microbial Fuel Cells Driven by Pseudomonas aeruginosa PPNB101 Palash Pan, Nandan Bhattacharyya This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8181546/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The rising demand for sustainable energy emphasizes microbial fuel cells as promising systems that couple bioelectricity generation with waste remediation. In this study, a cellulolytic and electroactive bacterium, Pseudomonas aeruginosa PPNB101, was isolated from industrially impacted soil and identified through biochemical characterization and 16S rRNA gene sequencing. The strain exhibited dual functionality, effectively degrading cellulose and transferring electrons extracellularly. The cellulase activity increased significantly from 2.10 ± 0.08 to 3.00 ± 0.12 mg·mL⁻¹ glucose (t (6) = 5.42, p < 0.01), with a strong growth-activity correlation (F (3, 18) = 24.7, p < 0.001). In parallel, phenazine-like metabolites accumulated progressively (F (4, 20) = 19.5, p < 0.001). A single-chamber, membrane-less MFC configured with an aluminum wire anode and graphite cathode achieved an open circuit voltage of 751 mV and a maximum power density of ~ 168 mW·m⁻² at the current density ~ 913 mA·m⁻², with V–I regression showing excellent fit (R² = 0.94, p < 0.001). COD removal reached 48.1% ± 2.3, significantly higher than the abiotic control, and Coulombic efficiency was 39.7% ± 1.8. Four MFCs in series delivered a voltage of 1.67–2.04 V over a prolonged duration to power a 2 V LED, whereas nine units produced ~ 6.5 V and intermittently powered a 6 V lamp. Effluent analysis indicated its potential as a nitrogen-rich organic fertilizer. These results highlight a sustainable approach for transforming cellulosic biomass into renewable energy through the use of low-cost and recyclable materials, underscoring its potential application in the valorization of agricultural residues. Bioelectricity Cellulase Coulombic efficiency Microbial fuel cells Organic fertilizer Power Density Pseudomonas Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8181546","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":562388652,"identity":"da50e96c-77a9-4dad-a879-0d46b549c15d","order_by":0,"name":"Palash Pan","email":"","orcid":"","institution":"Panskura Banamali College (Autonomous), P.O.– Panskura R.S","correspondingAuthor":false,"prefix":"","firstName":"Palash","middleName":"","lastName":"Pan","suffix":""},{"id":562388653,"identity":"febbd53a-8558-4642-9d5e-eaeb891f0ad5","order_by":1,"name":"Nandan 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PPNB101\u003c/strong\u003e\u003c/em\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Bioelectricity, Cellulase, Coulombic efficiency, Microbial fuel cells, Organic fertilizer, Power Density, Pseudomonas","lastPublishedDoi":"10.21203/rs.3.rs-8181546/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8181546/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe rising demand for sustainable energy emphasizes microbial fuel cells as promising systems that couple bioelectricity generation with waste remediation. In this study, a cellulolytic and electroactive bacterium, \u003cem\u003ePseudomonas aeruginosa\u003c/em\u003e PPNB101, was isolated from industrially impacted soil and identified through biochemical characterization and 16S rRNA gene sequencing. The strain exhibited dual functionality, effectively degrading cellulose and transferring electrons extracellularly. The cellulase activity increased significantly from 2.10\u0026thinsp;\u0026plusmn;\u0026thinsp;0.08 to 3.00\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12 mg\u0026middot;mL⁻\u0026sup1; glucose (t (6)\u0026thinsp;=\u0026thinsp;5.42, p\u0026thinsp;\u0026lt;\u0026thinsp;0.01), with a strong growth-activity correlation (F (3, 18)\u0026thinsp;=\u0026thinsp;24.7, p\u0026thinsp;\u0026lt;\u0026thinsp;0.001). In parallel, phenazine-like metabolites accumulated progressively (F (4, 20)\u0026thinsp;=\u0026thinsp;19.5, p\u0026thinsp;\u0026lt;\u0026thinsp;0.001). A single-chamber, membrane-less MFC configured with an aluminum wire anode and graphite cathode achieved an open circuit voltage of 751 mV and a maximum power density of ~\u0026thinsp;168 mW\u0026middot;m⁻\u0026sup2; at the current density\u0026thinsp;~\u0026thinsp;913 mA\u0026middot;m⁻\u0026sup2;, with V\u0026ndash;I regression showing excellent fit (R\u0026sup2; = 0.94, p\u0026thinsp;\u0026lt;\u0026thinsp;0.001). COD removal reached 48.1% \u0026plusmn; 2.3, significantly higher than the abiotic control, and Coulombic efficiency was 39.7% \u0026plusmn; 1.8. Four MFCs in series delivered a voltage of 1.67\u0026ndash;2.04 V over a prolonged duration to power a 2 V LED, whereas nine units produced\u0026thinsp;~\u0026thinsp;6.5 V and intermittently powered a 6 V lamp. Effluent analysis indicated its potential as a nitrogen-rich organic fertilizer. These results highlight a sustainable approach for transforming cellulosic biomass into renewable energy through the use of low-cost and recyclable materials, underscoring its potential application in the valorization of agricultural residues.\u003c/p\u003e","manuscriptTitle":"Bioelectricity Generation and System Scaling in Microbial Fuel Cells Driven by Pseudomonas aeruginosa PPNB101","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-12-19 09:20:18","doi":"10.21203/rs.3.rs-8181546/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"3b63f947-3d8a-4265-b6d5-255790b62798","owner":[],"postedDate":"December 19th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-01-07T07:24:39+00:00","versionOfRecord":[],"versionCreatedAt":"2025-12-19 09:20:18","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8181546","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8181546","identity":"rs-8181546","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}