High Expression of CXCL10/CXCR3 in Ventilator-Induced Lung Injury Caused by High Mechanical Power

High Expression of CXCL10/CXCR3 in Ventilator-Induced Lung Injury Caused by High Mechanical Power | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article High Expression of CXCL10/CXCR3 in Ventilator-Induced Lung Injury Caused by High Mechanical Power Yongpeng Xie, Hui Zheng, Zhifang Mou, Yanli Wang, Xiaomin Li This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-841117/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 07 Jan, 2022 Read the published version in BioMed Research International → Version 1 posted You are reading this latest preprint version Abstract Background : The energy delivered by a ventilator to the respiratory system in one minute is defined as mechanical power (MP). However, the effect of the ventilator-induced lung injury (VILI) in patients suffering of acute respiratory distress syndrome (ARDS) is still unknown. Our previous studies revealed that CXCL10 may be a potential biomarker of lung injury in ARDS. Therefore, the aim of this study was to compare the lung injury of rats and patients under different MP conditions to explore the involvement of CXCL10 and its receptor CXCR3 in VILI. Methods : Patients were divided into high mechanical power group (HMP) and low mechanical power group (LMP), while rats were assigned to the high mechanical power group (HMP), medium mechanical power group (MMP) and low mechanical power group (LMP). CXCL10 and CXCR3 plasma content in ARDS patients and rats under ventilation at different MP was measured, as well as their protein and mRNA expression in rat lungs. Results : CXCL10 and CXCR3 content in the plasma of ARDS patients in the HMP was significantly higher than that in the LMP. The increase of MP during mechanical ventilation in the rats gradually increased lung damage, and CXCL10 and CXCR3 level in rat plasma gradually increased with the increase of MP. CXCL10 and CXCR3 protein and mRNA expression in the HMP group and MMP group was significantly higher than that in the LMP group (P<0.05). More mast cells were present in the trachea, bronchus, blood vessels, and lymphatic system in the rat lungs of the HMP group and the number of mast cells in the HMP group (13.32±3.27) was significantly higher than that in the LMP group (3.25±0.29) (P＜0.05). Conclusion : The higher the MP, the more severe the lung injury, the higher CXCL10/CXCR3 expression. Therefore, CXCL10/CXCR3 might participate in VILI by mediating mast cell chemotaxis. Medical Genetics Critical Care & Emergency Medicine Health Policy acute respiratory distress syndrome ventilator-induced lung injury CXCL10 CXCR3 mast cells. Full Text Additional Declarations No competing interests reported. Supplementary Files Figue5CXCL10.jpg Figue5CXCR3.jpg Figue5GAPDH.jpg Cite Share Download PDF Status: Published Journal Publication published 07 Jan, 2022 Read the published version in BioMed Research International → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-841117","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":48151104,"identity":"bd8f1c17-8593-4b03-b2c8-d127dd1f6fe5","order_by":0,"name":"Yongpeng Xie","email":"","orcid":"","institution":"Lianyungang Clinical College of Nanjing Medical University, s Hospital of Lianyungang","correspondingAuthor":false,"prefix":"","firstName":"Yongpeng","middleName":"","lastName":"Xie","suffix":""},{"id":48151105,"identity":"e1e424b4-dfa9-4ff0-9fbb-39b952ab6eb1","order_by":1,"name":"Hui Zheng","email":"","orcid":"","institution":"Lianyungang 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syndrome, ventilator-induced lung injury, CXCL10, CXCR3, mast cells.","lastPublishedDoi":"10.21203/rs.3.rs-841117/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-841117/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground\u003c/strong\u003e: The energy delivered by a ventilator to the respiratory system in one minute is defined as mechanical power (MP). However, the effect of the ventilator-induced lung injury (VILI) in patients suffering of acute respiratory distress syndrome (ARDS) is still unknown. Our previous studies revealed that CXCL10 may be a potential biomarker of lung injury in ARDS. Therefore, the aim of this study was to compare the lung injury of rats and patients under different MP conditions to explore the involvement of CXCL10 and its receptor CXCR3 in VILI. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e: Patients were divided into high mechanical power group (HMP) and low mechanical power group (LMP), while rats were assigned to the high mechanical power group (HMP), medium mechanical power group (MMP) and low mechanical power group (LMP). CXCL10 and CXCR3 plasma content in ARDS patients and rats under ventilation at different MP was measured, as well as their protein and mRNA expression in rat lungs. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e: CXCL10 and CXCR3 content in the plasma of ARDS patients in the HMP was significantly higher than that in the LMP. The increase of MP during mechanical ventilation in the rats gradually increased lung damage, and CXCL10 and CXCR3 level in rat plasma gradually increased with the increase of MP. CXCL10 and CXCR3 protein and mRNA expression in the HMP group and MMP group was significantly higher than that in the LMP group (P\u0026lt;0.05). More mast cells were present in the trachea, bronchus, blood vessels, and lymphatic system in the rat lungs of the HMP group and the number of mast cells in the HMP group (13.32±3.27) was significantly higher than that in the LMP group (3.25±0.29) (P＜0.05). \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eConclusion\u003c/strong\u003e: The higher the MP, the more severe the lung injury, the higher CXCL10/CXCR3 expression. Therefore, CXCL10/CXCR3 might participate in VILI by mediating mast cell chemotaxis.\u003c/p\u003e","manuscriptTitle":"High Expression of CXCL10/CXCR3 in Ventilator-Induced Lung Injury Caused by High Mechanical Power","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2021-09-23 14:50:21","doi":"10.21203/rs.3.rs-841117/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"996d5b1e-d701-4af9-980e-00cff3fdfec7","owner":[],"postedDate":"September 23rd, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":7383751,"name":"Medical Genetics"},{"id":7383752,"name":"Critical Care \u0026 Emergency Medicine"},{"id":7383753,"name":"Health Policy"}],"tags":[],"updatedAt":"2022-01-07T22:22:49+00:00","versionOfRecord":{"articleIdentity":"rs-841117","link":"https://doi.org/10.1155/2022/6803154","journal":{"identity":"biomed-research-international","isVorOnly":true,"title":"BioMed Research International"},"publishedOn":"2022-01-07 22:22:49","publishedOnDateReadable":"January 7th, 2022"},"versionCreatedAt":"2021-09-23 14:50:21","video":"","vorDoi":"10.1155/2022/6803154","vorDoiUrl":"https://doi.org/10.1155/2022/6803154","workflowStages":[]},"version":"v1","identity":"rs-841117","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-841117","identity":"rs-841117","version":["v1"]},"buildId":"_2-kVJe1T_tPrBINL-cwx","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}