The study on functional compensation of soil microorganisms under nitrogen-only fertilization based on indigenous forest fungal pools

The study on functional compensation of soil microorganisms under nitrogen-only fertilization based on indigenous forest fungal pools | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 10 October 2025 V1 Latest version Share on The study on functional compensation of soil microorganisms under nitrogen-only fertilization based on indigenous forest fungal pools Authors : Qiancui Zhang , He Guandi 0000-0002-0056-1495 [email protected] , Zicheng Guo , Yeqing He , Tengbing He , and Siew Ling Lee Authors Info & Affiliations https://doi.org/10.22541/au.176007467.70583861/v1 114 views 82 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Long-term application of nitrogen fertilizer alone has led to reduced soil pH, imbalances in microbial communities, and the proliferation of harmful microorganisms in maize fields, which are key factors contributing to yield decline. Based on a four-year field experiment in Anshun City, Guizhou Province, China, this study employed a forest-derived indigenous microbial pool (T) to assess its potential in restoring soil microbial communities under nitrogen only fertilization (S) and balanced fertilization (B), using integrated metagenomic and metabolomic analyses. The results showed that the T treatment significantly increased soil microbial α diversity (p< 0.05). The relative abundance of the key functional group Pseudomonadota reached 44.33% under T, representing increases of 115% and 99% compared to S (20.63%) and B (22.31%), respectively. Functional genes related to carbon and nitrogen metabolism- ackA , gltB , and ureC -were significantly enrichedin the T treatment, accompanied by enhanced activities of glycolysis and anaerobic ammonia oxidation pathways. These findings indicate that the forest microbial pool has strong potential to compensate for functional losses in microbial communities caused by nitrogen only fertilization. Metabolomic analysis further revealed significant enrichment of plant resistance-related pathways, including phenylpropanoid biosynthesis products, along with notable increases in key metabolites such as GABA (+24%), L-tryptophan (+34%), and cellobiose (+3%). Notably, the T treatment exhibited a distinctive dominance of Candidatus Rokubacteria in nitrogen metabolism, with a relative abundance of 69%. Overall, the results demonstrate that the forest-derived indigenous microbial pool can effective lyrestore microbial ecological balance in soils degraded by nitrogen overuse and offers a theoretical foundation for developing targeted microbial-based soil amendments. Supplementary Material File (abstract.eps) Download 15.30 MB File (figure1.eps) Download 16.12 MB File (figure2.eps) Download 26.73 MB File (figure3.eps) Download 16.21 MB File (figure6.eps) Download 11.27 MB File (manuscrpit.docx) Download 30.16 MB Information & Authors Information Version history V1 Version 1 10 October 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords fertilized soil forest microorganisms microbial inoculant soil microbial community soil restoration Authors Affiliations Qiancui Zhang Guizhou University College of Agriculture View all articles by this author He Guandi 0000-0002-0056-1495 [email protected] Guizhou University College of Agriculture View all articles by this author Zicheng Guo Guizhou University College of Agriculture View all articles by this author Yeqing He Guizhou University College of Agriculture View all articles by this author Tengbing He Guizhou University College of Agriculture View all articles by this author Siew Ling Lee Universiti Teknologi Malaysia View all articles by this author Metrics & Citations Metrics Article Usage 114 views 82 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Qiancui Zhang, He Guandi, Zicheng Guo, et al. The study on functional compensation of soil microorganisms under nitrogen-only fertilization based on indigenous forest fungal pools. Authorea . 10 October 2025. DOI: https://doi.org/10.22541/au.176007467.70583861/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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