Influence of Thermal and Populational Variables on Locomotor Activity in Gromphadorhina portentosa

Influence of Thermal and Populational Variables on Locomotor Activity in Gromphadorhina portentosa | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Influence of Thermal and Populational Variables on Locomotor Activity in Gromphadorhina portentosa Eduardo Gracidas Reyes, Gerardo Ulises Díaz Arango This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8040039/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract For developing efficient cyborg cockroaches for search and rescue operations (SAR), characterizing Gromphadorhina portentosa is crucial for optimizing its potential as a biohybrid platform. This study examines how controlled thermal conditions and population factors, specifically gender and body length, affect key locomotor parameters of Gromphadorhina portentosa using a custom motion tracking and a temperature control systems. We analyzed the movement of 42 individuals across six temperature groups ranging from 18°C to 38°C. Mixed models and post-hoc analysis revealed non-linear thermal effects on locomotor performance, with an optimal activity peak at 30°C while minimums at 18°C and 34°C. Maximum acceleration, maximum velocity, and detention percentage showed the greatest sensitivity to temperature. Gender had minimal influence on temperature, while body length was moderately associated with total travel and average velocity. These findings enhance the current understanding of the behavioral and environmental effects on Gromphadorhina portentosa, contributing to the development of more efficient cyborg insect systems and highlighting the importance of environmental optimization in biobotic engineering. romphadorhina portentosa Biobotics Insect biomechanics Thermoregulation Temperature cyborg cockroaches Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8040039","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":540503282,"identity":"9c4d4454-35c0-4d61-8e8a-b8b64c71e960","order_by":0,"name":"Eduardo Gracidas Reyes","email":"data:image/png;base64,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","orcid":"","institution":"Universidad de las Américas Puebla","correspondingAuthor":true,"prefix":"","firstName":"Eduardo","middleName":"Gracidas","lastName":"Reyes","suffix":""},{"id":540503283,"identity":"47118d41-25cc-4a21-a9ab-463eff98b60b","order_by":1,"name":"Gerardo Ulises Díaz Arango","email":"","orcid":"","institution":"Universidad Veracruzana","correspondingAuthor":false,"prefix":"","firstName":"Gerardo","middleName":"Ulises Díaz","lastName":"Arango","suffix":""}],"badges":[],"createdAt":"2025-11-05 15:38:16","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-8040039/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8040039/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":95276679,"identity":"480dfca9-2834-4e06-92d5-b0a0f092e046","added_by":"auto","created_at":"2025-11-06 08:31:24","extension":"json","order_by":0,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":4366,"visible":true,"origin":"","legend":"","description":"","filename":"e42e398e5a214876bac2f3f318fb495b.json","url":"https://assets-eu.researchsquare.com/files/rs-8040039/v1/dc626822f689a5707c6e4da6.json"},{"id":95675275,"identity":"2f9e910e-a7f9-4331-9cde-fb8e58f347f2","added_by":"auto","created_at":"2025-11-11 18:23:29","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2658252,"visible":true,"origin":"","legend":"","description":"","filename":"SpringerNatureLaTeXTemplate.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8040039/v1_covered_1318e820-8173-471b-b24d-2cf621959399.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Influence of Thermal and Populational Variables on Locomotor Activity in Gromphadorhina portentosa","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"romphadorhina portentosa, Biobotics, Insect biomechanics, Thermoregulation, Temperature, cyborg cockroaches","lastPublishedDoi":"10.21203/rs.3.rs-8040039/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8040039/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"For developing efficient cyborg cockroaches for search and rescue operations (SAR), characterizing Gromphadorhina portentosa is crucial for optimizing its potential as a biohybrid platform. 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