Glycan chip based on structure switchable DNA linker for on-chip biosynthesis of cancer-associated complex glycans

:root { --font-inter: 'Inter', sans-serif, Symbol; --font-monospace: monospace; --font-sans-serif: sans-serif; } html, body, div, span, h1, h2, h3, h4, h5, h6, p, ol, ul, li, table, caption, tbody, tfoot, thead, tr, th, td {margin: 0;padding: 0;border: 0;font-size: 100%;font: inherit;vertical-align: baseline;} html {font-size: 14px;line-height: 1.5;} body {font-size: 14px;line-height: 1.5;font-family: var(--font-inter);} ol, ul {list-style: none;} blockquote, q {quotes: none;} blockquote:before, blockquote:after, q:before, q:after {content: '';content: none;} img {max-width: 100%;} table {border-collapse: collapse;border-spacing: 0;} b {font-weight: bold;} a {color: #2c71d6;text-decoration: none;} p {margin: 10px 0 10px 0;} .no-select {-o-user-select: none;-moz-user-select: none;-khtml-user-select: none;-webkit-user-select: none;user-select: none;} * {box-sizing: border-box;} @keyframes blink {0% {opacity: .1;}20% {opacity: 1;}100% {opacity: .1;}} .main-preloader{position:fixed;display: flex;top:0;bottom:0;left:0;right:0;align-items: center;justify-content: center;z-index:200000000;text-align:center;background:#F2F3F6;} .main-preloader.dark{background:#363636;} .main-preloader.hidden {display: none;} .main-preloader .mp-logo{height:100px;width:100px;} Glycan chip based on structure switchable DNA linker for on-chip biosynthesis of cancer-associated complex ... {"@context":"https://schema.org","@id":"https://www.protocols.io/#org","@type":"Organization","logo":{"@type":"ImageObject","height":"1550","url":"https://www.protocols.io/img/protocols-og.jpg","width":"1920"},"name":"protocols.io","url":"https://www.protocols.io/"} {"@context":"https://schema.org","@id":"https://www.protocols.io/#website","@type":"WebSite","name":"protocols.io","publisher":{"@id":"https://www.protocols.io/#org"},"url":"https://www.protocols.io/"} {"@context":"https://schema.org","@id":"https://www.protocols.io/view/glycan-chip-based-on-structure-switchable-dna-link-14egn9k16l5d/v1#webpage","@type":"WebPage","about":{"@id":"https://www.protocols.io/#org"},"description":"Glycan chip based on structure switchable DNA linker for on-chip biosynthesis of cancer-associated complex glycans\n. . Read full protocol, steps, and materia...","isPartOf":{"@id":"https://www.protocols.io/#website"},"name":"Glycan chip based on structure switchable DNA linker for on-chip biosynthesis of cancer-associated complex ...","url":"https://www.protocols.io/view/glycan-chip-based-on-structure-switchable-dna-link-14egn9k16l5d/v1"} {"@context":"https://schema.org","@id":"https://www.protocols.io/view/glycan-chip-based-on-structure-switchable-dna-link-14egn9k16l5d/v1#article","@type":"Article","author":{"@type":"Person","affiliation":"Dev","name":"Protocols Importer"},"dateModified":"2021-05-26T10:39:35Z","datePublished":"2021-05-26T10:39:35Z","description":"On-chip glycan biosynthesis is an effective strategy for preparing useful complex glycan sources and for preparing glycan-involved applications simultaneously. However, current methods have some limitations when analyzing biosynthesized glycans and optimizing enzymatic reactions, which could result in undefined glycan structures on a surface, leading to unequal and unreliable results. In this work, a novel glycan chip was developed by introducing a pH-responsive i-motif DNA linker to control the immobilization and isolation of glycans on chip surfaces in a pH-dependent manner. On-chip enzymatic glycosylations were optimized for uniform biosynthesis of cancer-associated Globo H hexasaccharide and its related complex glycans through stepwise quantitative analyses of isolated products from the surface. Successful interaction analyses of the anti-Globo H antibody and MCF-7 breast cancer cells with on-chip biosynthesized Globo H-related glycans demonstrated the feasibility of the structure-switchable DNA linker-based glycan chip platform for on-chip complex glycan biosynthesis and glycan-involved applications. ","headline":"Glycan chip based on structure switchable DNA linker for on-chip biosynthesis of cancer-associated complex ...","identifier":"10.21203/rs.3.pex-1350/v1","image":{"@type":"ImageObject","url":"https://files.protocols.io/webapp/images/q2ckeg/meta/protocolsio.png"},"keywords":"Biotechnology,complex glycan chip, on-chip enzymatic glycosylation, structure switchable DNA linker, cancer-associated glycans, glycan-cell interactions, pextocheck, chip complex glycan biosynthesi, chip glycan biosynthesi, complex glycan biosynthesi, biosynthesized glycan, novel glycan chip, chip enzymatic glycosylation, based glycan chip platform, related complex glycans through stepwise quantitative analysis, glycan chip platform, glycan chip, chip biosynthesis of cancer, preparing glycan, isolation of glycan, enzymatic glycosylation, preparing useful complex glycan source, undefined glycan structure, complex glycan, associated complex glycan, related complex glycan, useful complex glycan source, glycan, chip biosynthesi, related glycan, structure switchable dna linker, switchable dna linker, uniform biosynthesis of cancer, motif dna linker, optimizing enzymatic reaction, cancer cell, chip surface","mainEntityOfPage":{"@id":"https://www.protocols.io/view/glycan-chip-based-on-structure-switchable-dna-link-14egn9k16l5d/v1#webpage"},"publisher":{"@id":"https://www.protocols.io/#org"}} var WebappBundlePath = "https:\/\/files.protocols.io\/webapp\/bundles\/production-3c17661\/"; var AMPLITUDE_API_KEY="c250ed1d862cb9c01587e1f779a32c9";var FFMPEG_HOST="https://ffmpeg.protocols.io";var FILES_CDN_URL="https://files.protocols.io";var FILES_S3_BUCKET="protocols-io-files";var FILES_S3_PREFIX="external";var GA_MEASUREMENT_ID="G-33ZGV5CT0B";var GOOGLE_CLIENT_ID="182746271041-rdtjk0b1h9706r0fgpq1o2h55lgbo8un.apps.googleusercontent.com";var HAS_SSR= false ;var HOST_ROLE= 1 ;var HOST_ROLES={"HOST_DEV":2,"HOST_LOCAL":3,"HOST_PROD":1};var IMAGE_BASE_PATH="https://files.protocols.io/webapp/images/q2ckeg/";var IS_VPC_CLOUD= false ;var NATS_SERVER="wss://ws.protocols.io";var ORCID_CLIENT_ID="APP-7405TP4958GFSZXV";var ORCID_URL="https://orcid.org/oauth/authorize";var RECAPTCHA_SITE_ID="6LeMF6YUAAAAAOYzOqfc9Ml3PdTgIzO1oDdIwCYc";var SITE_URL="https://www.protocols.io";var SPRINGERNATURE_CLIENT_ID="protocolsio";var SPRINGERNATURE_REDIRECT="https://www.protocols.io/api/v1/auth/springernature/authcallback";var SPRINGERNATURE_URL="https://idp.springernature.com";var STRIPE_PUB_KEY="pk_live_51PfMxdRshFFmaQRVGeDsSX7NFFLMkMuqr17SxP6JcJuieglmQYHPgM7cz0XdO91IEVP9k4E4MC7PZB2ATyuuaOy800ivYpRURi";var UIPE="ccW9Kn73Dw3C4V1lovN9duN1d5BCqNRXHRUQiYoxsGA=";var VPC_ALLOW_REGENTS_LINKING= false ;var VPC_DISABLED_INVITE_COLLEAGUES= false ;var VPC_DISABLED_PUBLISHING= false ;var VPC_DISABLED_SIGN_UP= false ;var VPC_DISABLED_WORKSPACE_CREATION= false ;var VPC_EXTERNAL_STORAGES= null ;var VPC_FORWARDED_VIEW_URL_PREFIX="";var VPC_MAIN_CLOUD_URL="https://www.protocols.io";var VPC_REQUIRE_SIGN_IN= false ;var VPC_USE_MAIN_GLOBAL_SEARCH= false ;var WEBAPP_VERSION="production-3c17661";var YJS_WEBSOCKET_URL="wss://www.protocols.io";var __stripeGlobal= null ;var cookie_session_guid="81F55574B27211F197D20A58A9FEAC02";var gosessid="81F55379B27211F197D20A58A9FEAC02";var is_premium= false ;var loadedAmplitude= false ;var loadedGA= false ;var logged_in= false ;var session_guid="";var show_loader= true ;var user_theme="default"; var is_grecaptcha_loaded = false; if (!Element.prototype.matches) Element.prototype.matches = Element.prototype.msMatchesSelector; if (!Element.prototype.closest) Element.prototype.closest = function (selector) {var el = this; while (el) {if (el.matches(selector)) return el; el = el.parentElement;}};