Expression and significance of WT-1,P53,MMP-9 and TIMP-2 in ovarian papillary serous carcinoma

Objective:To investigate the expression and significance of WT-1,P53,matrix metalloproteinase-9(MMP-9)and tis-sue inhibitor of metalloproteinase-2(TIMP-2)in ovarian papillary serous carcinoma.Methods:WT-1,P53,MMP-9 and TIMP-2 were detected by S-P immunohistochemitry in 52 cases of ovarian papillary serous carcinoma,and compared with 15 cases of primary peritoneal papillary serous carcinoma and 11 cases of uterine papillary serous carcinoma.Results:The positive expres-sion rates of WT-1 in ovarian papillary serous carcinoma,peritoneal papillary serous carcinoma and uterine papillary serous car-cinoma were 96.2%,93.3%,and 36.4%,respectively.The positive expression rates of P53 were 78.8%,80%,and 72.7%,respec-tively.The positive expression rates of MMP-9 and TIMP-2 were 90.4%,93.3%,100%,and 92.3%,93.3%,100%,respectively.The area integrated optical density(AIOD/μm)2of WT-1、P53、MMP-9 and TIMP-2 analyzed by image analyzer in low differentiation group of ovarian serous carcinoma were high than those in high differentiation group(P0.01).The AIOD/μm2 of WT-1 in o-varian papillary serous carcinoma and primary peritoneal papillary serous carcinoma were high than that in uterine papillary serous carcinoma(P0.01).Conclusion:The expression of WT-1,P53,MMP-9 and TIMP-2 in low differentiation group of ovarian papillary serous carcinoma are higher than those in high differentiation group.The overexpression of WT-1,P53,MMP-9 and TIMP-2 are related to the degree of differentiation of the ovarian papillary serous carcinoma.The detection of WT-1 may be a useful marker for distinguishing ovarian papillary serous carcinoma from uterine papillary serous carcinoma.