Transcription characteristics of chemokine receptors in endometrium and endometrial tissues

Objective:To investigate the expression of chemokine receptor in eupotic and ectopic endometrial tissues of women with endometriosis, and in endometrium of women without endometriosis.Methods:Normal endometrium, eutopic endometrium and endometriotic tissues were obtained from patients with endometriosis at laparoscopy. Total RNA was then extracted using the TRIzol reagent. The expression of chemokine receptors in these tissues were analyzed by way of semi-quantitative reverse transcriptase-polymerase chain reaction.Results:Compared to normal endometrium, the eutopic endometrium expressed significantly more CCR6, CCR8, CCR9 and CX3CR1(P0.05). Compared to the eutopic endometrium, the endometriotic tissue expressed apparently more CCR4, CCR8, CCR9 and CXCR1(P0.05).Conclusion:The chemokine receptors were expressed differently in normal endometrium, eutopic endometrium and endometriotic tissues. The expression difference between normal endometrium and the eutopic endometrium may play role in the onset of endometriosis. The expression difference between the eutopic endometrium and the endometriotic tissues may participate in the development of endometriosis.