Circular RNA as new serum metabolic biomarkers in patients with Premature Ovarian Insufficiency

Abstract Objective Quantitative Real-time PCR (qPCR) is used to detect the differential expression of Circular RNAs in patients of Premature Ovarian Insufficiency (POI), in order to explore the new biomarkers of POI that can be detected from blood as soon as possible. Methods The study collected plasma samples from 30 patients in POI group and 30 normal people group that meet the inclusion criteria, who visited the gynecology clinic of The First Affiliated Hospital of Guangzhou University of Chinese Medicine from July 2019 to December 2020. Then circRNAs in plasmawere extracted for qPCR validation. Results 1. qPCR technology was performed on hsa_circRNA_008901 and hsa_circRNA_403959, and it was found that the levels of both were considerably downregulated in POI group. Clinical evaluation showed that both hsa_circRNA_008901 and hsa_circRNA_403959 have good diagnostic value for POI. 2. According to miRNA Regulatory Element (MRE) analysis, the predicted target miRNAs of hsa_circRNA_008901 are: hsa-miR-548c-3p, hsa-miR-924, hsa-miR-4677-5p, hsa-miR-6786 -3p and hsa-miR-7974; the predicted target miRNAs of hsa_circRNA_403959 are: hsa-miR-1207-5p, hsa-miR-4691-5p, hsa-miR-4763-3p, hsa-miR-6807-5p and hsa -miR-7160-5p. Conclusion Compared with the normal group, the expression levels of hsa_circRNA_008901 and hsa_circRNA_403959 in the POI group were downregulated, suggesting that these two circRNAs may be potential biomarkers of POI. Bioinformatics analysis indicated that hsa_circRNA_008901 and hsa_circRNA_403959 may regulate their binding miRNA through the action form of “molecular sponge", and then regulate the signaling pathway regulated by miRNA, and ultimately affect the disease progression of POI.