Exploring Anticancer and Antituberculosis Activities of Pt(II) Quinazoline Complexes Derived from EMNEDAQZHO ((E)-2-methyl-3-(1-(naphthalen-1-yl)ethylideneamino)quinazolin-4(3H)-one): Insights and Applications  

Exploring Anticancer and Antituberculosis Activities of Pt(II) Quinazoline Complexes Derived from EMNEDAQZHO ((E)-2-methyl-3-(1-(naphthalen-1-yl)ethylideneamino)quinazolin-4(3H)-one): Insights and Applications | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Exploring Anticancer and Antituberculosis Activities of Pt(II) Quinazoline Complexes Derived from EMNEDAQZHO ((E)-2-methyl-3-(1-(naphthalen-1-yl)ethylideneamino)quinazolin-4(3H)-one): Insights and Applications Sheeba Sheeba This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7357781/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract In vitro cytotoxicity assessment has become a critical aspect of developing therapeutic agents for a wide array of human diseases, including cancer, autoimmune disorders, and infectious ailments like tuberculosis (TB). Metal complexes, especially those based on platinum, have garnered substantial attention in cancer treatment due to their notable efficacy, typified by cisplatin's ability to induce filamentous growth. Cisplatin, an inorganic heavy metal complex, shares mechanistic similarities with certain cell-cycle-phase non-specific alkylating agents, functioning via the formation of interstrand DNA cross-links and DNA adducts, thereby impeding DNA, RNA, and protein synthesis, primarily in cancer cells. TB remains a significant global health challenge, necessitating the development of novel vaccines and drugs to combat its spread. The rational development of antitubercular agents hinges on a comprehensive understanding of the genetics, physiology, and host interactions of Mycobacterium tuberculosis. In this study, platinum metal complexes derived from (E)-2-methyl-3-(1-(naphthalen-1-yl)ethylidene amino) quinazolin-4(3H)-one [EMNEDAQZHO] were synthesized and evaluated for their potential as anticancer and antituberculosis agents. Meticulous molecule selection, protein data bank (PDB) optimization, and multi-component reaction methods were employed for synthesis. Characterization of the resultant complexes was conducted using various analytical techniques, encompassing IR, TLC, NMR, XRD, TGA, Mass spectrum, and physicochemical parameter analysis. The prioritized molecules underwent in vitro assays to assess their anticancer activity via the MTT assay and antituberculosis activity using M. tuberculosis strain MTCC 300 as standards. The findings reveal promising potential for platinum metal complexes derived from quinazolinone Schiff bases [EMNEDAQZHO] as both anticancer and antituberculosis agents, as evidenced by their observed inhibitory effects in in vitro cytotoxicity assays and evaluations against M. tuberculosis strain MTCC 300. This study underscores the therapeutic promise of these novel platinum metal complexes and emphasizes the necessity for further investigations to elucidate their mechanisms of action and therapeutic efficacy in both preclinical and clinical contexts. Biological sciences/Biochemistry Biological sciences/Cancer Biological sciences/Chemical biology Physical sciences/Chemistry Biological sciences/Computational biology and bioinformatics Biological sciences/Drug discovery Biological sciences/Microbiology In vitro cytotoxicity assessment Anticancer therapy Antituberculosis therapy Metal complexes Platinum-based compounds Cisplatin Mechanism of action Mycobacterium tuberculosis. Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryData2025.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7357781","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":509556689,"identity":"91730fc9-fea4-4a6c-ae0e-5e70132a87f1","order_by":0,"name":"Sheeba Sheeba","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABFElEQVRIiWNgGAWjYDACCQYGZiCVwMDMxsaQ+OefHEjwwAOitXxsOGAM1pJAlBYGNjbGmQ0HEhsYIFycQH5288HHhW11efztbGmPeXfcSZ8fdvgh0BY7Od0G7FoM7hxLNp7ZdrhY4jDbcWPeM89yN95OMwBqSTY2O4BDi0SOmTRvG9A9h9nbpHnYmHM3zk4AaTmQuA2HFvkZ+d9/87bVJc6Hakk3nJ3+Aa8Whhs5bMy8bcyJGw6zHZMEujBBXjoHvy0GN9KMpWecO5y48TBbmsSHM2mGG6RzCg4kGOD2i/yM5IefC8rqEuedP2YmkVBhIy8/O33zhw8VdnK4tGCxF6zSgFjlYHsbSFE9CkbBKBgFIwEAACIeZx/lAVKxAAAAAElFTkSuQmCC","orcid":"","institution":"","correspondingAuthor":true,"prefix":"","firstName":"Sheeba","middleName":"","lastName":"Sheeba","suffix":""}],"badges":[],"createdAt":"2025-08-12 16:23:17","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7357781/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7357781/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":91997546,"identity":"596d9dc5-6c28-49e2-ab14-cd5edc5a2ea3","added_by":"auto","created_at":"2025-09-23 13:53:59","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":851626,"visible":true,"origin":"","legend":"","description":"","filename":"RevisedManuscript2025.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7357781/v1_covered_efc0d9a1-9a6e-4f56-b758-35606d892671.pdf"},{"id":90851585,"identity":"380c0c29-a212-4c81-8acf-7f96e15d5a2b","added_by":"auto","created_at":"2025-09-09 03:23:29","extension":"pdf","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":643661,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryData2025.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7357781/v1/f28d89a935398ea6121f8833.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Exploring Anticancer and Antituberculosis Activities of Pt(II) Quinazoline Complexes Derived from EMNEDAQZHO ((E)-2-methyl-3-(1-(naphthalen-1-yl)ethylideneamino)quinazolin-4(3H)-one): Insights and Applications ","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"In vitro cytotoxicity assessment, Anticancer therapy, Antituberculosis therapy, Metal complexes, Platinum-based compounds, Cisplatin, Mechanism of action, Mycobacterium tuberculosis.","lastPublishedDoi":"10.21203/rs.3.rs-7357781/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7357781/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eIn vitro cytotoxicity assessment has become a critical aspect of developing therapeutic agents for a wide array of human diseases, including cancer, autoimmune disorders, and infectious ailments like tuberculosis (TB). Metal complexes, especially those based on platinum, have garnered substantial attention in cancer treatment due to their notable efficacy, typified by cisplatin's ability to induce filamentous growth. Cisplatin, an inorganic heavy metal complex, shares mechanistic similarities with certain cell-cycle-phase non-specific alkylating agents, functioning via the formation of interstrand DNA cross-links and DNA adducts, thereby impeding DNA, RNA, and protein synthesis, primarily in cancer cells. TB remains a significant global health challenge, necessitating the development of novel vaccines and drugs to combat its spread. The rational development of antitubercular agents hinges on a comprehensive understanding of the genetics, physiology, and host interactions of Mycobacterium tuberculosis.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eIn this study, platinum metal complexes derived from (E)-2-methyl-3-(1-(naphthalen-1-yl)ethylidene amino) quinazolin-4(3H)-one [EMNEDAQZHO] were synthesized and evaluated for their potential as anticancer and antituberculosis agents. Meticulous molecule selection, protein data bank (PDB) optimization, and multi-component reaction methods were employed for synthesis. Characterization of the resultant complexes was conducted using various analytical techniques, encompassing IR, TLC, NMR, XRD, TGA, Mass spectrum, and physicochemical parameter analysis. The prioritized molecules underwent in vitro assays to assess their anticancer activity via the MTT assay and antituberculosis activity using M. tuberculosis strain MTCC 300 as standards. The findings reveal promising potential for platinum metal complexes derived from quinazolinone Schiff bases [EMNEDAQZHO] as both anticancer and antituberculosis agents, as evidenced by their observed inhibitory effects in in vitro cytotoxicity assays and evaluations against M. tuberculosis strain MTCC 300. This study underscores the therapeutic promise of these novel platinum metal complexes and emphasizes the necessity for further investigations to elucidate their mechanisms of action and therapeutic efficacy in both preclinical and clinical contexts.\u003c/p\u003e","manuscriptTitle":"Exploring Anticancer and Antituberculosis Activities of Pt(II) Quinazoline Complexes Derived from EMNEDAQZHO ((E)-2-methyl-3-(1-(naphthalen-1-yl)ethylideneamino)quinazolin-4(3H)-one): Insights and Applications ","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-09-09 03:23:21","doi":"10.21203/rs.3.rs-7357781/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b96ca8f0-2ab5-4e53-adbe-ad2002dde4d4","owner":[],"postedDate":"September 9th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":54119094,"name":"Biological sciences/Biochemistry"},{"id":54119095,"name":"Biological sciences/Cancer"},{"id":54119096,"name":"Biological sciences/Chemical biology"},{"id":54119097,"name":"Physical sciences/Chemistry"},{"id":54119098,"name":"Biological sciences/Computational biology and bioinformatics"},{"id":54119099,"name":"Biological sciences/Drug discovery"},{"id":54119100,"name":"Biological sciences/Microbiology"}],"tags":[],"updatedAt":"2025-09-23T13:53:21+00:00","versionOfRecord":[],"versionCreatedAt":"2025-09-09 03:23:21","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7357781","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7357781","identity":"rs-7357781","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}