Endogenous N/OFQ Regulates Diabetic Peripheral Neuropathy via Membrane metallo-endopeptidase Enzymes

Endogenous N/OFQ Regulates Diabetic Peripheral Neuropathy via Membrane metallo-endopeptidase Enzymes | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 10 April 2025 V1 Latest version Share on Endogenous N/OFQ Regulates Diabetic Peripheral Neuropathy via Membrane metallo-endopeptidase Enzymes Authors : Yi Han 0000-0003-3385-9789 [email protected] , Yuan-jing Qin , Jing Li , Chun-yan Zhang , Peng Zhang , Tian-qi Wang , Qi-xing Yang , and Lin-Zhong Zhang Authors Info & Affiliations https://doi.org/10.22541/au.174430103.39753194/v1 203 views 253 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Abstract： Background and Purpose: Diabetic peripheral neuropathy (DPN), a common chronic complication in type 1 and type 2 diabetes due to metabolic dysregulation and nerve ischemia, was the focus. This research aimed to explore the therapeutic potential of UFP-101, an NOPR antagonist, in DPN. Experimental Approach: Thirty male Sprague - Dawley rats were divided into five groups: control, DPN model, and three DPN groups treated with different doses of UFP-101 (0.01mg/kg, 0.03mg/kg, 0.1mg/kg). Human umbilical vein endothelial cells were also split into six groups with different treatments related to glucose levels, N/OFQ, UFP-101, and inhibitors. Key Results: High - dose UFP-101 (0.03mg/kg and 0.1mg/kg) had positive effects. It decreased Tail flicking latency, reduced sciatic nerve stimulation thresholds, and restored femoral artery blood flow. Molecularly, it downregulated PKC phosphorylation, decreased MME protein expression, increased serum CNP levels, and improved nerve fiber integrity. In cell culture, Go6976 and LCZ696 reduced MME expression, and UFP - 101, Go6976, and LCZ696 increased CNP levels. Conclusions and Implications: UFP-101 mitigates DPN progression by modulating the PKC - MME - CNP signaling axis, indicating its potential as a targeted DPN treatment. Supplementary Material File (r12-qin-bjp.doc) Download 291.73 KB Information & Authors Information Version history V1 Version 1 10 April 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords diabetes gpcr neurodegeneration/neuroprotection Authors Affiliations Yi Han 0000-0003-3385-9789 [email protected] Shanxi Medical University Second Affiliated Hospital View all articles by this author Yuan-jing Qin Shanxi Medical University View all articles by this author Jing Li China Railway Group Ltd View all articles by this author Chun-yan Zhang Linfen People's Hospital View all articles by this author Peng Zhang Shanxi Medical University Second Affiliated Hospital View all articles by this author Tian-qi Wang Shanxi Medical University View all articles by this author Qi-xing Yang Linfen People's Hospital View all articles by this author Lin-Zhong Zhang Shanxi Medical University Second Affiliated Hospital View all articles by this author Metrics & Citations Metrics Article Usage 203 views 253 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Yi Han, Yuan-jing Qin, Jing Li, et al. Endogenous N/OFQ Regulates Diabetic Peripheral Neuropathy via Membrane metallo-endopeptidase Enzymes. Authorea . 10 April 2025. DOI: https://doi.org/10.22541/au.174430103.39753194/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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