Mechanism Research on Treatment of Guizhi Fuling Pills for Endometriosis in Rats

Objective:To study the influence of Guizhi Fuling pills(GFP) on pain and matrix metalloproteinases expression in rats with endometriosis.Method: Rat model of endometriosis(EMS) was established.The model rats were divided into the model group,ibuprofen group(0.054 g · kg-1),danazol group(0.036 g · kg-1),and GFP low,high dose groups(0.256,1.024 g · kg-1).The treatment in each group was lasted for 4 weeks.Plasma 6-ketone prostaglandin F1α(6-keto-PGF1α),beta endorphins(beta EP) and thromboxane B2(TXB2) were determined by radioimmunoassay.Tissue of uterus endometriosis was obtained,and lesions and size were evaluated.The expression of matrix metalloproteinases-2(MMP-2) and matrix metalloproteinases-9(MMP-9) in ectopic endometrium was determined.Result: GFP could reduce the volume of ectopic endometrium significantly(P0.01).Two doses of GFP could increase plasma 6-keto-PGF1α,beta EP level,and reduce TXB2 level(P0.01).Two doses of GFP could reduce the expression of MMP-2 and MMP-9(P0.05,P0.01).Conclusion: GFP inhibits the growth of ectopic endometrium.The mechanism may be related to inhibition of the expression of MMP-2 and MMP-9.The pain relieving of GFP may be obtained through the balance prostaglandin level,alleviation of spasms of smooth muscle,and elevation of endorphins.