Seroprevalence, Assessement of KAP, and Public Health Implication of Bovine Brucellosis in West Hararghe Zone of Oromia Regional State, Eastern Ethiopia

Seroprevalence, Assessement of KAP, and Public Health Implication of Bovine Brucellosis in West Hararghe Zone of Oromia Regional State, Eastern Ethiopia | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Seroprevalence, Assessement of KAP, and Public Health Implication of Bovine Brucellosis in West Hararghe Zone of Oromia Regional State, Eastern Ethiopia moges Abate Aklilu, Daniel Tamirat weldemariam This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6406555/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Bovine brucellosis is a bacterial disease that affects both cattle and humans. This study aimed to estimate the seroprevalence of bovine brucellosis in four districts of West Harerghe zone, Eastern Ethiopia, using mRBPT and I-ELISA tests. The study was conducted from July 2024 to February 2025 G.C. using a cross-sectional design and random sampling. A total of 384 cattle of local breeds were sampled from two extensive and two semi-intensive management systems. The overall individual animal seroprevalence was 3.9% by mRBPT and 3.1% by I-ELISA. The seroprevalence was higher in the extensive (2.9%) than in the semi-intensive (0.3%) management system. Larger herd sizes, older age groups, females, lowland agroecology, and pastoral production systems were associated with higher seroprevalence. A structured questionnaire was used to interview 100 cattle owners to assess their (KAP) regarding bovine brucellosis and its public health importance. The results showed that 80% of the respondents had no information about brucellosis, 73% had risk practices, and 83% had negative attitudes, which could facilitate the transmission of brucellosis between cattle and humans. Our findings suggest that bovine brucellosis is prevalent in the study area and poses a significant zoonotic risk. We recommend implementing control measures to prevent the spread of the disease and to raise awareness among cattle owners and consumers. Attitude Brucellosis Knowledge Practices Seroprevalence Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6406555","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":447725039,"identity":"03170dec-55c1-47bb-a73c-a745f62959b9","order_by":0,"name":"moges Abate Aklilu","email":"","orcid":"","institution":"Haramaya University","correspondingAuthor":false,"prefix":"","firstName":"moges","middleName":"Abate","lastName":"Aklilu","suffix":""},{"id":447725040,"identity":"91085b64-259f-41e6-a111-644a815def64","order_by":1,"name":"Daniel Tamirat weldemariam","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABAUlEQVRIie3PMUsDMRTA8RcC51Kvjt7kVzjpJFT9KgkHTtc6uDgGCnFpO9fFfgVdOr+QoUtL10B2J4cUlyIBm6uDU+9uFMyfEELIj0cAYrG/GB4WMACK6MIpOWlD8EASrmYVoS0I/JBOT3eqmyaSLtdKO98fdmcs1/2vxUVKgbhteZxkqyFDJe8ezg1jejC1l5ICzZ4Xx0mOZY5KaC4MQz0YWxJIQk/ryOYjR/TffG640Fdje9tMTJiCCfJXU4CGneWNJDNhyloW/G31DmoibCEpGdX+Jd2UPffob/jL8v7T7by9nj+NlNvWkN/OGACR1YmINu9DXQybb/k4FovF/lV7AWVlCFPY97QAAAAASUVORK5CYII=","orcid":"","institution":"Haramaya University","correspondingAuthor":true,"prefix":"","firstName":"Daniel","middleName":"Tamirat","lastName":"weldemariam","suffix":""}],"badges":[],"createdAt":"2025-04-08 22:38:09","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6406555/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6406555/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":82058103,"identity":"6e824690-a0ab-4db2-ae83-f8f2fd313846","added_by":"auto","created_at":"2025-05-06 10:53:54","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":791783,"visible":true,"origin":"","legend":"","description":"","filename":"DaniandMOGESFINALamendedwithallcontents.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6406555/v1_covered_33eef1d8-fe98-44ee-b410-a7a93e8ea15b.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Seroprevalence, Assessement of KAP, and Public Health Implication of Bovine Brucellosis in West Hararghe Zone of Oromia Regional State, Eastern Ethiopia","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Attitude, Brucellosis, Knowledge, Practices, Seroprevalence","lastPublishedDoi":"10.21203/rs.3.rs-6406555/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6406555/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBovine brucellosis is a bacterial disease that affects both cattle and humans. This study aimed to estimate the seroprevalence of bovine brucellosis in four districts of West Harerghe zone, Eastern Ethiopia, using mRBPT and I-ELISA tests. The study was conducted from July 2024 to February 2025 G.C. using a cross-sectional design and random sampling. A total of 384 cattle of local breeds were sampled from two extensive and two semi-intensive management systems. The overall individual animal seroprevalence was 3.9% by mRBPT and 3.1% by I-ELISA. The seroprevalence was higher in the extensive (2.9%) than in the semi-intensive (0.3%) management system. Larger herd sizes, older age groups, females, lowland agroecology, and pastoral production systems were associated with higher seroprevalence. A structured questionnaire was used to interview 100 cattle owners to assess their (KAP) regarding bovine brucellosis and its public health importance. The results showed that 80% of the respondents had no information about brucellosis, 73% had risk practices, and 83% had negative attitudes, which could facilitate the transmission of brucellosis between cattle and humans. Our findings suggest that bovine brucellosis is prevalent in the study area and poses a significant zoonotic risk. We recommend implementing control measures to prevent the spread of the disease and to raise awareness among cattle owners and consumers.\u003c/p\u003e","manuscriptTitle":"Seroprevalence, Assessement of KAP, and Public Health Implication of Bovine Brucellosis in West Hararghe Zone of Oromia Regional State, Eastern Ethiopia","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-05-02 06:30:37","doi":"10.21203/rs.3.rs-6406555/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"702f35bc-d551-4ae1-9e81-f59965ed29ac","owner":[],"postedDate":"May 2nd, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-05-06T10:53:18+00:00","versionOfRecord":[],"versionCreatedAt":"2025-05-02 06:30:37","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6406555","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6406555","identity":"rs-6406555","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}