Evaluation of Differential Expression of MUC20 and sprr1B in Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorder

Evaluation of Differential Expression of MUC20 and sprr1B in Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorder | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Evaluation of Differential Expression of MUC20 and sprr1B in Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorder Dr.Payal Panwar, Dr.Priyanka singh, Dr Shalini Gupta, Dr Uma Shanker pal, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9433889/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Aim To evaluate the expression of MUC20 and SPRR1B in Oral Squamous Cell Carcinoma (OSCC), Oral Potentially Malignant Disorders (OPMDs), and normal oral mucosa, and to correlate their expression with clinicopathological parameters. Materials and Methods: A total of 108 formalin-fixed paraffin-embedded tissue samples were included, comprising 36 OSCC, 36 OPMDs (OSMF, OED, OLP), and 36 normal oral mucosa. Immunohistochemical analysis was performed using primary antibodies against MUC20 and SPRR1B. Expression levels were scored based on intensity and proportion of positively stained cells. Statistical analysis was conducted using SPSS v23, with significance set at p < 0.05. Results MUC20 and SPRR1B expression was significantly higher in OSCC compared to OPMDs and normal mucosa (p < 0.001). In OPMDs, MUC20 expression was highest in mild dysplasia, while SPRR1B expression increased progressively with dysplasia severity. In OSCC, higher MUC20 and SPRR1B levels were associated with poorer histological differentiation. No significant correlation was observed between expression and OSCC clinical stage. Conclusion Both MUC20 and SPRR1B show progressive upregulation from normal mucosa to OPMDs and OSCC, with expression correlating to histopathological grade and dysplasia severity. These findings suggest their potential utility as biomarkers for early detection and prognostic evaluation in oral carcinogenesis. MUC20 SPRR1B Oral Squamous Cell Carcinoma Oral Potentially Malignant Disorders Immunohistochemistry Biomarker Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9433889","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":626470469,"identity":"d71abf0d-e138-42a6-b058-e06a9ec57bbc","order_by":0,"name":"Dr.Payal Panwar","email":"","orcid":"","institution":"King George's Medical University","correspondingAuthor":false,"prefix":"Dr.","firstName":"Payal","middleName":"","lastName":"Panwar","suffix":""},{"id":626470472,"identity":"8a5f0b68-a291-4f52-a670-093143827f26","order_by":1,"name":"Dr.Priyanka singh","email":"","orcid":"","institution":"King George's Medical University","correspondingAuthor":false,"prefix":"Dr.","firstName":"Priyanka","middleName":"","lastName":"singh","suffix":""},{"id":626470473,"identity":"4b69b1c3-37ce-48f6-8994-1da494c5e2ef","order_by":2,"name":"Dr Shalini Gupta","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAuklEQVRIiWNgGAWjYDACCQbGAwwGNkAWY+MBYrUwALWkgbQ0kKKF4TCYTZwW+dnNDw4XFJy3W9t+GGhLjU00QS0Gd44ZHJ5hcDt525lEoJZjabkNBLVIJBgc5gFqMTsA1MLYcJiwFvkZ6R+AWs4lm51/SKQWhhs5IFsO2JndINYWgxs5BUAtyQlmN4C2JBDjF6DDNj7m+WNnb3Y+/eGDDzU2RDgMChLBKhOIVQ4C9qQoHgWjYBSMghEGACohSTpmbndQAAAAAElFTkSuQmCC","orcid":"","institution":"King George's Medical University","correspondingAuthor":true,"prefix":"Dr","firstName":"Shalini","middleName":"","lastName":"Gupta","suffix":""},{"id":626470475,"identity":"0ad7568e-a98a-483e-a767-88cb3250f951","order_by":3,"name":"Dr Uma Shanker pal","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"Dr","firstName":"Uma","middleName":"Shanker","lastName":"pal","suffix":""},{"id":626470476,"identity":"1df61c7b-207e-40ad-a6f6-85a77b8a56da","order_by":4,"name":"Dr. Vandana Singh","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"Dr.","firstName":"Vandana","middleName":"","lastName":"Singh","suffix":""},{"id":626470477,"identity":"4c972b04-b91f-4720-8f9c-f98841059aca","order_by":5,"name":"Dr. Sameer Gupta","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"Dr.","firstName":"Sameer","middleName":"","lastName":"Gupta","suffix":""}],"badges":[],"createdAt":"2026-04-16 06:23:28","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-9433889/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9433889/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":108125289,"identity":"6b5fa227-5cae-4393-b033-6c842ffeb543","added_by":"auto","created_at":"2026-04-29 15:10:55","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1477092,"visible":true,"origin":"","legend":"","description":"","filename":"thesis15.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9433889/v1_covered_fd3a7052-ef24-47e4-b149-d94c3f531479.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"\u003cp\u003eEvaluation of Differential Expression of MUC20 and sprr1B in Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorder\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"MUC20, SPRR1B, Oral Squamous Cell Carcinoma, Oral Potentially Malignant Disorders, Immunohistochemistry, Biomarker","lastPublishedDoi":"10.21203/rs.3.rs-9433889/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9433889/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eAim\u003c/h2\u003e \u003cp\u003eTo evaluate the expression of MUC20 and SPRR1B in Oral Squamous Cell Carcinoma (OSCC), Oral Potentially Malignant Disorders (OPMDs), and normal oral mucosa, and to correlate their expression with clinicopathological parameters. Materials and Methods: A total of 108 formalin-fixed paraffin-embedded tissue samples were included, comprising 36 OSCC, 36 OPMDs (OSMF, OED, OLP), and 36 normal oral mucosa. Immunohistochemical analysis was performed using primary antibodies against MUC20 and SPRR1B. Expression levels were scored based on intensity and proportion of positively stained cells. Statistical analysis was conducted using SPSS v23, with significance set at p\u0026thinsp;\u0026lt;\u0026thinsp;0.05.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eMUC20 and SPRR1B expression was significantly higher in OSCC compared to OPMDs and normal mucosa (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001). In OPMDs, MUC20 expression was highest in mild dysplasia, while SPRR1B expression increased progressively with dysplasia severity. In OSCC, higher MUC20 and SPRR1B levels were associated with poorer histological differentiation. No significant correlation was observed between expression and OSCC clinical stage.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e \u003cp\u003eBoth MUC20 and SPRR1B show progressive upregulation from normal mucosa to OPMDs and OSCC, with expression correlating to histopathological grade and dysplasia severity. These findings suggest their potential utility as biomarkers for early detection and prognostic evaluation in oral carcinogenesis.\u003c/p\u003e","manuscriptTitle":"Evaluation of Differential Expression of MUC20 and sprr1B in Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorder","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-04-27 02:49:05","doi":"10.21203/rs.3.rs-9433889/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"d43bf6c5-03b4-4837-a06b-ba27598a5a30","owner":[],"postedDate":"April 27th, 2026","published":true,"recentEditorialEvents":[{"type":"decision","content":"Rejected","date":"2026-04-29T14:57:40+00:00","index":"","fulltext":""}],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-04-29T15:09:50+00:00","versionOfRecord":[],"versionCreatedAt":"2026-04-27 02:49:05","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-9433889","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-9433889","identity":"rs-9433889","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}