Algorithms for coding and decoding images and video data in DNA plasmid in Escherichia coli

Algorithms for coding and decoding images and video data in DNA plasmid in Escherichia coli | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Algorithms for coding and decoding images and video data in DNA plasmid in Escherichia coli Jorge Juarez, Raquel Diaz, Maria del Rayo Guevara, Anabel Sanchez-Sanchez, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6846379/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The need for storage has increased with the development of social networks and streaming platforms. The storage requirement has gone from gigabytes to zettabytes. The use of networks requires thousands of images to perform the learning that allows them to be used as classification systems in health, facilitating the diagnosis of diseases. This situation has led research centers and institutions to join the DNA Data Storage Alliance and seek new information storage strategies. As a result, it has been suggested that DNA can store data and image information innovatively due to its density, stability, and scalability. In this sense, the present work develops the necessary bioinformatics, nomenclature, and an innovative algorithm that allows storing images and video in any DNA sequence, using, as an example, the plasmid pJK635 that can be inserted into the bacterium Escherichia coli and using these plasmids as storage of image information. The results show that 2K quality images in black and white, color, and even a video sequence can be stored inside a plasmid. The developed algorithm facilitates the storage of information by converting the pixel value of the images into a sequence of DNA nucleotides that can be stored within the plasmid, including the image size information, the file type, and the set of pixels in the image, all bounded between primers. Conversely, the image can be reconstructed by passing the nucleotides to their respective pixels for display on a computer. Escherichia coli Plasmid Image database video nucleotides pixels DNA information Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6846379","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":512626196,"identity":"cfe07e22-1c33-4ec1-8e6b-d3bb12156189","order_by":0,"name":"Jorge Juarez","email":"","orcid":"","institution":"National Institute of Astrophysics, Optics and Electronics","correspondingAuthor":false,"prefix":"","firstName":"Jorge","middleName":"","lastName":"Juarez","suffix":""},{"id":512626197,"identity":"d5da72c2-9994-4d54-8c0d-a4a29bbe5b2d","order_by":1,"name":"Raquel Diaz","email":"","orcid":"","institution":"National Institute of Astrophysics, 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