Cellulose extraction and quantification from eucalyptus leaves in the laboratory

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This protocol aims. Read full protocol, steps, and materials on protocols.io","isPartOf":{"@id":"https://www.protocols.io/#website"},"name":"Cellulose extraction and quantification from eucalyptus leaves in the laboratory\n V.1","url":"https://www.protocols.io/view/cellulose-extraction-and-quantification-from-eucal-kqdg3qjbzv25/v1"} {"@context":"https://schema.org","@id":"https://www.protocols.io/view/cellulose-extraction-and-quantification-from-eucal-kqdg3qjbzv25/v1#article","@type":"Article","author":{"@type":"Person","affiliation":"Dev","name":"Protocols Importer"},"dateModified":"2024-01-26T15:59:49Z","datePublished":"2024-01-26T15:59:49Z","description":"This protocol aims to extract cellulose from plant material using a nitric acid digestion and quantification using spectrophotometry. This protocol has been modified from existing protocols so that extraction of cellulose can be performed in 2 mL microcentrifuge tubes using ground plant material as the starting product. Key stages of the procedure include the nitric acid digestion and removal of breakdown products where approximately 100 samples can be digested in a day. The procedure is concluded with a conversion of cellulose to glucose, and finally quantification using spectrophotometry where approximately 200 samples can be quantified in a day. ","headline":"Cellulose extraction and quantification from eucalyptus leaves in the laboratory\n V.1","identifier":"10.21203/rs.3.pex-2401/v1","image":{"@type":"ImageObject","url":"https://files.protocols.io/webapp/images/q2ckeg/meta/protocolsio.png"},"keywords":"Environmental sciences,Plant sciences,Eucalyptus,flammability,fire,drought,structural hydrocarbon,leaves,vegetation,leaf material,plant traits.,cellulose extraction,quantification from eucalyptus leaf,cellulose from plant material,conversion of cellulose,cellulose,eucalyptus leaf,eucalyptus,using spectrophotometry,nitric acid digestion,using ground plant material,using nitric acid,ground plant material as the starting pr...,extraction,nitric acid,plant material, cellulose extraction, quantification from eucalyptus leaf, cellulose from plant material, conversion of cellulose, cellulose, eucalyptus leaf, eucalyptus, using spectrophotometry, nitric acid digestion, using ground plant material, using nitric acid, ground plant material as the starting product, extraction, nitric acid, plant material","mainEntityOfPage":{"@id":"https://www.protocols.io/view/cellulose-extraction-and-quantification-from-eucal-kqdg3qjbzv25/v1#webpage"},"publisher":{"@id":"https://www.protocols.io/#org"}} var WebappBundlePath = "https:\/\/files.protocols.io\/webapp\/bundles\/production-f458b32\/"; var AMPLITUDE_API_KEY="c250ed1d862cb9c01587e1f779a32c9";var FFMPEG_HOST="https://ffmpeg.protocols.io";var FILES_CDN_URL="https://files.protocols.io";var FILES_S3_BUCKET="protocols-io-files";var FILES_S3_PREFIX="external";var GA_MEASUREMENT_ID="G-33ZGV5CT0B";var GOOGLE_CLIENT_ID="182746271041-rdtjk0b1h9706r0fgpq1o2h55lgbo8un.apps.googleusercontent.com";var HAS_SSR= false ;var HOST_ROLE= 1 ;var HOST_ROLES={"HOST_DEV":2,"HOST_LOCAL":3,"HOST_PROD":1};var IMAGE_BASE_PATH="https://files.protocols.io/webapp/images/q2ckeg/";var NATS_SERVER="wss://ws.protocols.io";var ORCID_CLIENT_ID="APP-7405TP4958GFSZXV";var ORCID_URL="https://orcid.org/oauth/authorize";var RECAPTCHA_SITE_ID="6LeMF6YUAAAAAOYzOqfc9Ml3PdTgIzO1oDdIwCYc";var SITE_URL="https://www.protocols.io";var SPRINGERNATURE_CLIENT_ID="protocolsio";var SPRINGERNATURE_REDIRECT="https://www.protocols.io/api/v1/auth/springernature/authcallback";var SPRINGERNATURE_URL="https://idp.springernature.com";var STRIPE_PUB_KEY="pk_live_51PfMxdRshFFmaQRVGeDsSX7NFFLMkMuqr17SxP6JcJuieglmQYHPgM7cz0XdO91IEVP9k4E4MC7PZB2ATyuuaOy800ivYpRURi";var UIPE="ccW9Kn73Dw3C4V1lovN9dhdEvizyoN5hpBOzwM24c7Y=";var VPC_ALLOW_REGENTS_LINKING= false ;var VPC_DISABLED_INVITE_COLLEAGUES= false ;var VPC_DISABLED_PUBLISHING= false ;var VPC_DISABLED_SIGN_UP= false ;var VPC_DISABLED_WORKSPACE_CREATION= false ;var VPC_EXTERNAL_STORAGES= null ;var VPC_FORWARDED_VIEW_URL_PREFIX="";var VPC_MAIN_CLOUD_URL="https://www.protocols.io";var VPC_REQUIRE_SIGN_IN= false ;var VPC_USE_MAIN_GLOBAL_SEARCH= false ;var WEBAPP_VERSION="production-f458b32";var YJS_WEBSOCKET_URL="wss://www.protocols.io";var __stripeGlobal= null ;var cookie_session_guid="AC99F0298BBE11F18B910A58A9FEAC02";var gosessid="AC99EE788BBE11F18B910A58A9FEAC02";var is_premium= false ;var loadedAmplitude= false ;var loadedGA= false ;var logged_in= false ;var session_guid="";var show_loader= true ;var user_theme="default"; var is_grecaptcha_loaded = false; if (!Element.prototype.matches) Element.prototype.matches = Element.prototype.msMatchesSelector; if (!Element.prototype.closest) Element.prototype.closest = function (selector) {var el = this; while (el) {if (el.matches(selector)) return el; el = el.parentElement;}};