Inducible TgfbR1 and Pten deletion in a novel model of tongue carcinogenesis and chemoprevention

Head and neck squamous cell carcinoma (HNSCC) is a significant public health problem, with a need for novel approaches to chemoprevention and treatment. Preclinical models that recapitulate molecular alterations that occur in clinical HNSCC patients are needed to better understand molecular and immune mechanisms of HNSCC carcinogenesis, chemoprevention and efficacy of treatment. We optimized a mouse model of tongue carcinogenesis with discrete quantifiable tumors via conditional deletion of Tgfβr1 and Pten by intralingual injection of tamoxifen. We characterized the localized immune tumor microenvironment, metastasis, systemic immune responses, associated with tongue tumor development. We further determined the efficacy of tongue cancer chemoprevention using dietary administration of black raspberries (BRB). Three Intralingual injections of 500ug tamoxifen to transgenic K14 Cre , floxed Tgfbr1 , Pten (2cKO) knock out mice resulted in tongue tumors with histological and molecular profiles, and lymph node metastasis similar to clinical HNSCC tumors. Bcl2 , Bcl-xl , Egfr , Ki -67, and Mmp9 , were significantly upregulated in tongue tumors compared to surrounding epithelial tissue. CD4 + and CD8 + T cells in tumor draining lymph nodes and tumors displayed increased surface CTLA4 expression, suggestive of impaired T cell activation and enhanced regulatory T cell activity. BRB administration resulted in reduced tumor growth, enhanced T cell infiltration to the tongue tumor microenvironment and robust anti-tumoral CD8 + cytotoxic T cell activity characterized by greater granzyme B and perforin expression. Our results demonstrate that intralingual injection of tamoxifen in Tgfβr1/Pten 2cKO mice results in discrete quantifiable tumors suitable for chemoprevention and therapy of experimental HNSCC.