Tracking spatio-temporal dynamics of early immune responses to an intranasal OMV-based pneumococcal vaccine in mice

Tracking spatio-temporal dynamics of early immune responses to an intranasal OMV-based pneumococcal vaccine in mice | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Tracking spatio-temporal dynamics of early immune responses to an intranasal OMV-based pneumococcal vaccine in mice Sajida Kanwal, Shaina To, Rienke Uijen, Rona Roverts, Bram van Cranenbroek, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7982436/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Outer membrane vesicle (OMV)-based vaccines elicit strong immune responses and have emerged as a versatile platform for targeting multiple pathogens, yet the mechanisms underlying their efficacy remain incompletely understood. Here, we investigated the early immune response following intranasal administration of an OMV-based pneumococcal vaccine in mice. Using in vivo bioluminescence imaging, spectral flow cytometry, and high-resolution microscopy, we tracked OMV biodistribution and immune activation throughout the respiratory tract from 1 to 72h post vaccination. OMVs persisted in the nasal cavity for up to 48 h and rapidly recruited Ly6Ghi neutrophils and myeloid-derived suppressor cells, followed by activation of local T cells. In the lungs, alveolar macrophages and plasmacytoid dendritic cells emerged as early responders. OMV exposure also induced costimulatory molecule expression across multiple myeloid cell subsets. Together, these findings reveal distinct spatio-temporal patterns of innate and adaptive immune activation at mucosal sites. Our multimodal analysis provides mechanistic insight into OMV-induced mucosal immunity and underscores their potential as a versatile platform for vaccine development. Biological sciences/Immunology/Antigen processing and presentation/Antigen-presenting cells Biological sciences/Immunology/Vaccines/Adjuvants Biological sciences/Immunology/Mucosal immunology Biological sciences/Immunology/Innate immune cells Biological sciences/Immunology/Adaptive immunity Full Text Additional Declarations Yes there is potential Competing Interest. Conflict of interests are in Abera Bioscience AB that aims to exploit the presented OMV technology and have patented this vaccine platform. Supplementary Files Video1.mp4 An animation showing volumetric FIB/SEM slices of the cell of interest and a corresponding 3D ultrastructural visualization Supplementarydata.docx Tracking spatio-temporal dynamics of early immune responses to an intranasal OMV-based pneumococcal vaccine in mice_Supplementary info VideoS1.mp4 2D FIB-SEM animation Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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